Abstract:Background: Abies webbiana commonly known as Talispatra in Bengali and Hindi, Talispatram in Sanskrit and Indian Silver Fir in English. This is a large, tall, evergreen tree occurring in the Himalayan region from Kashmir to Assam in India. It comes under the Family: Pinaceae. The present study was designed for isolation of flavonoid from ethyl acetate extract of A. webbiana leaves and assessed their toxic effect on liver and kidney. Materials and Methods: The isolation of flavonoid using different chroma togra… Show more
“…2S). The data obtained is in close agreement to the data Selvaraj et al [23], Adil et al [24] and Yadav et al [25].Fig. 7Chemical structure of quercetin.Fig.…”
The galls of Pistacia integerrima are utilized in folk medicines for the treatment of cough, asthma, dysentery, liver disorders and for snake bite. A number of biological active compounds like alkaloids, flavonoids, tannins, saponins and sterols from leaf, stem, bark, galls and fruit of this plant have been isolated. A number of authors have attempted to evaluate the medicinal potentials of this plant. Owing to the numerous ethno medicinal uses Pistacia integerrima the present study was aimed to isolate bioactive phenolic compounds from this plant and evaluate its antioxidant and enzyme inhibitory potentials against acetylcholine esterase (AChE) and butyryl cholinesterase (BChE). The ethyl acetate fraction was highly potent in scavenging the DPPH and ABTS free radicals (86.07 ± 0.92% and 83.50 ± 1.03% respectively) and inhibiting the selected enzymes (80.80 ± 2.45 and 82.56 ± 0.65 against AChE and BChE respectively). Two compounds, quercetin and pyrogallol were isolated for the first time from this plant. The isolated compounds were characterized by HPLC, FTIR and 1H-NMR. From the results it was concluded that this plant can be useful in releaving the oxidative stress and in the treatment neurological disorders.
“…2S). The data obtained is in close agreement to the data Selvaraj et al [23], Adil et al [24] and Yadav et al [25].Fig. 7Chemical structure of quercetin.Fig.…”
The galls of Pistacia integerrima are utilized in folk medicines for the treatment of cough, asthma, dysentery, liver disorders and for snake bite. A number of biological active compounds like alkaloids, flavonoids, tannins, saponins and sterols from leaf, stem, bark, galls and fruit of this plant have been isolated. A number of authors have attempted to evaluate the medicinal potentials of this plant. Owing to the numerous ethno medicinal uses Pistacia integerrima the present study was aimed to isolate bioactive phenolic compounds from this plant and evaluate its antioxidant and enzyme inhibitory potentials against acetylcholine esterase (AChE) and butyryl cholinesterase (BChE). The ethyl acetate fraction was highly potent in scavenging the DPPH and ABTS free radicals (86.07 ± 0.92% and 83.50 ± 1.03% respectively) and inhibiting the selected enzymes (80.80 ± 2.45 and 82.56 ± 0.65 against AChE and BChE respectively). Two compounds, quercetin and pyrogallol were isolated for the first time from this plant. The isolated compounds were characterized by HPLC, FTIR and 1H-NMR. From the results it was concluded that this plant can be useful in releaving the oxidative stress and in the treatment neurological disorders.
“…Abies webbiana have been successfully applied in pharmacological study and treatment due to their content of bioactive constituents. Abies have been regarded to have anti tumours, antimicrobial, anti ulcer, anti inflammatory and dental nervous system activities, while it has been considered as an antioxidant due to its chemical constituents contents, despite a long tradition of used in neurological disorders, no systemic pharmacological work has ever been carried out on Abbies webbiana to validate its traditional claims [24]. Therefore it was considered worthwhile to evaluate ethanol extract and fraction of Abies webbiana parts for various neuropharmacologist activities [25].…”
Aim: The aim of this study was to evaluate the antimicrobial and cytotoxic effect of Abies webbiana ethanolic extract based preparation of mouthwash and to compare these effects between different concentrations.
Background: Abies webbiana leaves have been used in traditional siddha and ayurveda systems of medicine for common ailments such as cough, loss of appetite, indigestion, mental disorders, rheumatism, bronchitis, pulmonary infection, asthma, antiseptic and decongestant. Abies webbiana commonly known as talispatra belongs to family Pinaceae. The plant is distributed throughout India, mostly in the Himalayan region from Kashmir to Assam states.
Materials and Methods: The preparation of Abies webbiana extract was done and antimicrobial activity of Abies webbiana were tested with Streptococcus mutans, Candida albicans, Enterococcus faecalis, Staphylococcus aureus through agar well diffusion method. Each bacterial culture was spread on the Luria-Bertani agar plates. Dried extract of Abies webbiana, dissolved in autoclave distilled water to make 25mg/mL, 50mg/mL, 100mg/mL. Incubated for 24 hours at 37o C and diameter of zone of inhibition were recorded in millimeter and compared with standard amoxicillin. Cytotoxic activity of Abies webbiana was assessed using brine shrimp lethality assay. A total of 10 nauplii were added into the three replicates of each concentration of the prepared extract. After the period of 24 hours the remaining brine shrimp were counted.
Results: The results of the study revealed that the the Abies webbiana formulation based mouthwash is having significantly higher antimicrobial activity than the antibiotic against S. aureus at all the tested concentrations whereas against E. faecalis the antimicrobial effect was significantly lower than the antibiotic. Against S. mutans, higher concentrations of the mouthwash had the antimicrobial effect comparable to that of the antibiotic. The antimicrobial effect against C. albicans was very less for all the tested concentrations of the mouthwash and the antibiotic. This mouthwash also possessed cytotoxic effects on nauplii which were within normal limits and it was found that the nauplii alive was directly proportional to the concentrations except in the concentration of 40μl.
Conclusion: Within the limitations of the current study, ethanolic extract of Abies webbiana based preparation of mouthwash shows the antimicrobial activity almost comparable to amoxicillin. It was also revealed that it possesses less cytotoxic effect against brine shrimp which are within normal limits.
“…A. webbiana leaf has been reported to exhibit antibacterial, antifungal, mast cell stabilizing, anxiolytic, antitumour, anti inflammatory, antitussive anti fertility, antispasmodic properties, central nervous system depressant activity and effective against hypoglycemia rheumatism [18,20,22]. Bronchodilation and antiplatelet activities of A. Webbiana were investigated and evaluated for the antioxidant and antimicrobial activity of A. Webbiana extract [23,24]. In addition, effect of A. webbiana leaf extract on bleeding time and inflammation was analysed [18,22,25].…”
Background: In ayurveda, many natural plant components are used to treat inflammatory conditions for centuries with less side effects. Different parts of Abies webbiana plant are used in ayurveda for many diseases. However, very few studies have been conducted to evaluate pharmacological effects of Abies webbiana.
Aim: The objective of this in vitro study was to analyse and compare the anti-inflammatory properties of A. webbiana at various concentrations using albumin denaturation assay.
Methodology: A ready made powder of A. webbiana was mixed with 100 ml of distilled water and boiled for 10 mins the extract was obtained by filtration. The anti-inflammatory activity was done using albumin denaturation assay and Diclofenac sodium was used as a positive control. Serial dilution from 10 µL, 20 µL, 30 µL, 40 µL and 50 µL was performed for A. webbiana and the reference drug reaction mixture was prepared for albumin denaturation assay. All the data was analysed statistically using SPSS version 23 (IBM). Unpaired t test was used for comparison with the positive control and one-way Anova followed by Tukey’s post hoc test were used for the comparison between different concentrations.
Results: The current study revealed that A. webbiana extract had potent anti-inflammatory activity at all the tested concentrations but was significantly lesser when compared to the standard (p<0.05). It was also evident that the absorbance rate of A. webbiana significantly increased with the increase in concentration of the extract (p<0.05). However, the absorbance rate at 50 µL of both standard and A. webbiana were almost similar.
Conclusion: It can be concluded from the present findings that the leaves of A. webbiana possess concentration-dependent anti-inflammatory property but is comparatively lesser than Diclofenac sodium.
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