Isolation of Fluorescent Pseudomonas spp. from Diverse Agro-Ecosystems of India and Characterization of their PGPR Traits

Kumar, G. Praveen; Desai, Suseelendra; Amalraj, E. Leo Daniel; Reddy, Gopal

doi:10.3923/bj.2015.13.24

Cited by 12 publications

(6 citation statements)

References 34 publications

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“…Among the four functional clusters analyzed in this paper (pseudomonads, spore-forming bacteria, actinobacteria, and mesophilic bacteria), pseudomonads were the group with the highest capacity, being all the selected isolates able to produce NH 4 + and NO 2 − /NO 3 − , and this result confirmed some literature reports. For example, Kumar et al [22] studied 75 isolates of Pseudomonas and found that all were NH 4 + producers, although at different levels (weak, moderate, or high). Moreover, Bacillus isolates showed a high degree of ammonium production (75%) and nitrification (60%).…”

Section: Discussionmentioning

confidence: 99%

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Isolation, Screening, and Characterization of Plant-Growth-Promoting Bacteria from Durum Wheat Rhizosphere to Improve N and P Nutrient Use Efficiency

et al. 2019

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The main goal of this paper was to select promising microorganisms which could potentially act as plant-growth-promoting bacteria (PGPB) for durum wheat of Foggia County. At this scope, a new statistical framework, based on multivariate analyses and the evaluation of the statistical distribution of each trait, was used. Four hundred and seventy-four isolates were isolated from the rhizosphere of durum wheat in Foggia County and preliminarily screened as a function of four target indices (ammonium production, siderophores production, P-solubilization, and nitrification). After this step, the number of strains was reduced and the remaining isolates were tested through a quantitative approach, to assess the production of IAA (indole acetic acid), P-mineralization, and nitrification. In this second step, the cut-off was based on the whole population trend by evaluating for each trait the medians and quartiles. As a result, 16 promising isolates were selected and identified by 16S rDNA sequencing (Bacillus, Pseudomonas, Stenotrophomonas, and Lysinibacillus). The last step of this research was a preliminary validation in a growth chamber on eight strains. As screening and simple indices, two quantitative measures were chosen. The main result was the selection of at least three isolates (6P, 20P, and 25A) for a future field validation. They increased biomass and height by respectively 50% and 25%.

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Section: Discussionmentioning

confidence: 99%

“…The experiment was done as reported previously [22]. Bacteria were inoculated in test tubes containing Peptone Water medium [23].…”

Section: Methodsmentioning

confidence: 99%

Isolation, Screening, and Characterization of Plant-Growth-Promoting Bacteria from Durum Wheat Rhizosphere to Improve N and P Nutrient Use Efficiency

et al. 2019

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“…Ammonia production by bacterial cultures was assayed in a peptone water medium (Peptone 1%, NaCl 0.5%), and incubated for 48 h at 37 • C in a shaker incubator. Ammonium accumulation was tested by adding 0.1 mL Nessler's reagent and observed for the development of yellow-brown color [24].…”

Section: In Vitro Analysis Of Plant Growth-promoting Traitsmentioning

confidence: 99%

Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

et al. 2021

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Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits invitro. Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified as Bacillus mojavensis and Bacillus cereus, respectively. A compatibility test between the isolates RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse conditions. Treatments included the application of MMRH22, RHPR20, their consortium (RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains was varied and was found to enhance the iron content up to 97%. This study revealed that treatments with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient deficiencies in plants and humans.

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“…Some of the isolates exhibited a strong ammonia producing ability. HCN production have been reported to play an essential role in the biological control of pathogenic fungi and deleterious rhizobacteria (Kumar et al, 2015). The possible use of siderophores as biopesticides paved special interest to study Pseudomonas (Wilson, 1997).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Pseudomonas Isolates From Diverse Agroecologies for Their Plant Growth Promoting Traits

Pinisetty¹,

Desai²,

Sultana³

et al. 2022

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Microorganisms contribute to soil health by improving growth and productivity of crops. The huge diversity of the genus Pseudomonas for their plant growth promoting traits is still under-exploited. Hence, 50 isolates of Pseudomonas from various agro-ecologies were assessed for their plant growth promoting traits. All the isolates were screened for production of growth hormones, exo-polysaccharides, siderophores, hydrolytic enzymes and bio-film. Among the 26% of IAA producers, P84 has shown the highest activity (39.73 µg mL-1). The highest EPS production was exhibited by P105 (60.58µg mL-1) and the least recorded was 2.93 µg mL-1 by P103. In case of ammonia production, ten isolates were strong, eighteen were moderate, fifteen isolates were weak and seven isolates were non producers of ammonia. The ability to produce HCN was exhibited by six isolates. Out of 50 isolates, nine isolates have shown clear zones for chitin/chitosan modifying enzymes. The efficient isolates were further assessed for growth promotion and disease suppression.

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Isolation of Fluorescent Pseudomonas spp. from Diverse Agro-Ecosystems of India and Characterization of their PGPR Traits

Cited by 12 publications

References 34 publications

Isolation, Screening, and Characterization of Plant-Growth-Promoting Bacteria from Durum Wheat Rhizosphere to Improve N and P Nutrient Use Efficiency

Isolation, Screening, and Characterization of Plant-Growth-Promoting Bacteria from Durum Wheat Rhizosphere to Improve N and P Nutrient Use Efficiency

Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

Characterization of Pseudomonas Isolates From Diverse Agroecologies for Their Plant Growth Promoting Traits

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