Isolation of Foot-and-Mouth Disease Virus from Japanese Black Cattle in Miyazaki Prefecture, Japan, 2000.

Abstract

ABSTRACT. Four outbreaks of foot-and-mouth disease (FMD) occurred from March to May 2000 in Miyazaki and Hokkaido Prefectures, Japan. FMD virus isolation was achieved by sampling probang materials from Japanese Black cattle in the third case found in Miyazaki Prefecture. The probang materials were inoculated to bovine kidney (BK) and bovine thyroid cell cultures. CPE was observed in the BK at two days post-inoculation. Specific amplified DNA segments for FMD virus (FMDV) were detected by reverse transcriptase polymerase chain reaction in the culture fluid. The FMDV was identified as type O by enzyme-linked immunosorbent assay (ELISA) for antigen detection and the nucleotide sequence encoding the VP1 was determined. This FMDV is a strain that is widespread in Pan-Asia and was designated as O/JPN/2000 by the World Reference Laboratory of the Pirbright Institute, England. This report marks the first isolation of FMDV in Japan. KEY WORDS: FMDV, O/JPN/2000, VP1.

Isolation and Characterization of a Parapoxvirus from Sheep with Papular Stomatitis

“…Therefore, at first, to deny FMD virus (FMDV), reverse transcriptase-PCR, antigen detection enzyme-linked immunosorbent assay (ELISA), and antibody detection ELISA for FMDV were carried out using mixed scabs from 7 sheep and each serum from 10 sheep as described previously 16 .…”

Foot-and-Mouth Disease Virus Antigen Detection Enzyme-Linked Immunosorbent Assay Using Multiserotype-Reactive Monoclonal Antibodies

“…The other disadvantage of IS-ELISA is that the manual recommends sampling vesicular fluid or tissue and culture fluid, but such samples may not be available in preclinical and/or subclinical diagnoses. For instance, the cattle in the 2000 FMD outbreak in Japan did not show clear vesicles (6,8,16). These problems would be solved if plasma and/or saliva could be used as the sample.…”

Availability of a fetal goat tongue cell line ZZ-R 127 for isolation of Foot-and-mouth disease virus (FMDV) from clinical samples collected from animals experimentally infected with FMDV

“…12 Pearson chi-square test and Student's t-test were used for analyzing the statistical significance of the differences in virus isolation rates between the ZZ-R 127 and the IB-RS-2 cells and in virus titers in the ZZ-R 127 and the IB-RS-2 cells, respectively. Table 1 In total, 292 and 144 samples were collected from the experimentally infected goats and pigs, respectively.…”

“…At the National Institute of Animal Health, Japan (NIAH), primary bovine kidney cells and IB-RS-2 cells are routinely used for diagnostic tests for FMD. 6,12 A previous study 2 reported that the fetal goat tongue cell line ZZ-R 127 was highly sensitive for the isolation of FMDV. The study evaluated its availability for FMDV isolation using cell culture supernatants and epithelial suspensions of various FMDV strains.…”