Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides

Benimeli, Claudia Susana; Amoroso, Maria Julia del R.; Chaile, Adriana P; Castro, Guillermo R.

doi:10.1016/s0960-8524(03)00061-0

Cited by 97 publications

(75 citation statements)

References 14 publications

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“…This fact is important for bioremediation purposes since complete pesticide degradation or mineralization is desirable and avoids the presence of more toxic or recalcitrant biotransformationderived compounds. Similar results were obtained by Benimeli et al (2003) which reported that the aquatic Streptomycete strain M7 was able to remove aldrin from medium, however, no intermediary compound originating from pesticide degradation was found in the culture broth. Cellular lysis did not increase pesticide concentration in the medium, so the authors suggested that aldrin was removed by metabolic reactions or by bioaccumulation into cell insoluble components.…”

Section: Resultssupporting

confidence: 88%

Isolation of Brazilian marine fungi capable of growing on DDD pesticide

et al. 2010

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The fungi Aspergillus sydowii Ce15, Aspergillus sydowii Ce19, Aspergillus sydowii Gc12, Bionectria sp. Ce5, Penicillium miczynskii Gc5, Penicillium raistrickii Ce16 and Trichoderma sp. Gc1, isolated from marine sponges Geodia corticostylifera and Chelonaplysylla erecta, were evaluated for their ability to grow in the presence of DDD pesticide. Increasing concentrations of DDD pesticide, i.e., 5.0 mg (1.56 × 10⁻¹² mmol), 10.0 mg (3.12 × 10⁻²) mmol) and 15.0 mg (4.68 × 10⁻² mmol) in solid and liquid culture media were tested. The fungi Trichoderma sp. Gc1 and Penicillium miczynskii Gc5 were able to grow in the presence of up to 15.0 mg of DDD, suggesting their potential for biodegradation. A 100% degradation of DDD was attained in liquid culture medium when Trichoderma sp. Gc1 was previously cultivated for 5 days and supplemented with 5.0 mg of DDD in the presence of hydrogen peroxide. However, the quantitative analysis showed that DDD was accumulated on mycelium and biodegradation level reached a maximum value of 58% after 14 days.

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Section: Resultssupporting

confidence: 88%

Isolation of Brazilian marine fungi capable of growing on DDD pesticide

et al. 2010

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“…It is important to note that neither the pesticide nor its potential toxic intermediaries (Lee et al 2006) had an inhibiting effect on bacterial growth, since significant differences in bacterial growth with and without MTX were not observed (P [ 0.05) (data not shown). The strains were not able to grow in MM containing L-asparagine as a sole carbon source (Benimeli et al 2003), and there was no evidence of microbial growth in control cultures without added MTX (data not shown). These results are very relevant, specially taking into account that there is little updated information about MTX degradation by actinobacteria.…”

Section: Resultsmentioning

confidence: 99%

“…A3, A6, A12, A14, and M7 were previously isolated from sediments and soil samples contaminated with several organochlorine pesticides (OPs) (Benimeli et al 2003;Fuentes et al 2010). Taxonomic identification of these strains has been confirmed by amplification and partial sequencing of their 16S rDNA genes [GenBank database, accession numbers: AY45953 (M7) (Benimeli et al 2007), GU085104 (A3), GQ867051 (A6), GQ867056 (A12), and GU085105 (A14) (Fuentes et al 2010)].…”

Section: Microorganisms and Culture Mediamentioning

confidence: 99%

“…These microorganisms have a great potential for the biodegradation of organic and inorganic toxic compounds, and several studies have demonstrated oxidation and partial dechlorination and dealkylation of aldrin, DDT and herbicides like metolachlor and atrazine, particularly in Streptomyces (Liu et al 1990;Radosevich et al 1995), the main genus of actinobacteria present in soils (Xu et al 1996). In previous studies, Benimeli et al (2003Benimeli et al ( , 2006Benimeli et al ( , 2007 and Fuentes et al (2010Fuentes et al ( , 2011 isolated and selected wild-type Streptomyces spp. strains which were able to tolerate and remove lindane (c-hexachlorocyclohexane), another OP, from culture media and soil, as pure and mixed cultures.…”

Section: Introductionmentioning

confidence: 99%

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Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Fuentes

Álvarez

Sáez

et al. 2013

Int. J. Environ. Sci. Technol.

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Methoxychlor is an organochlorine pesticide used worldwide against several insect pests, resulting in human exposure. This pesticide mimics endocrine hormone functions, interfering with normal endocrine activity in humans and wildlife. For this reason, it is imperative to develop methods to remove this pesticide from the environment, and though, bioremediation using microorganisms results as an excellent strategy. Five Streptomyces spp. strains previously isolated from organochlorine-polluted sites and capable to grow and remove methoxychlor were combined as different mixed cultures to increase methoxychlor removal. From the 39 consortia tested, one consortium (Streptomyces spp. A6, A12, A14, M7) was selected because of its high pesticide removal and specific dechlorinase activity to be assayed on slurry and soil systems. This consortium showed higher biomass values (8.3 9 10 6 ± 5.7 9 10 5 CFU mL -1 ) and methoxychlor removal (56.2 ± 2.3 %) on enriched slurry than in nonenriched slurry (7.3 9 10 5 ± 1.2 9 10 5 CFU mL -1 and 45.6 ± 7.4 % of pesticide removal). In soil systems, Streptomyces consortium showed higher growth (1.0 9 10 11 ± 5.0 9 10 10 CFU g -1 ) than in enriched slurry, although differences in methoxychlor removal between both culture conditions were not statistically significant. Therefore, the selected Streptomyces consortium may be suitable for the development of in situ (soil) and ex situ (slurry bioreactor) bioremediation methods because of their potential to remove methoxychlor from different systems.

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“…M7 was previously isolated from wastewater sediment samples of a copper filter plant, a pesticides contaminated site [32]. The microorganism was cultured in a soil extract broth (SE) prepared according to [33].…”

Section: Microorganisms and Culture Mediummentioning

confidence: 99%

Temperature and pH effect on lindane removal by Streptomyces sp. M7 in soil extract

Benimeli

González

Chaile

et al. 2007

J. Basic Microbiol.

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This work was conducted to study the removal of gamma-hexachlorocyclohexane (lindane) in a soil extract liquid medium (SE) by Streptomyces sp. M7 and to determine the influence of pH and temperature on bacterial growth and pesticide removal in this medium. When Streptomyces sp. M7 was cultured in SE supplemented with lindane 100 microg l(-1 )at different initial pH, the maximum growth was observed at pH 7 and the microorganism was not able to grow at pH 5 and 9; the highest pesticide removal (70.4%) by Streptomyces sp. M7 was noted at an initial pH of 7 at 4 weeks of incubation. The maximum removal (70% approximately) was observed when the microorganism was incubated in SE at 30 degrees C; although the optimal temperature for Streptomyces sp. M7 growth, with and without lindane, was 25 degrees C, and for the pesticide removal was 30 degrees C. The results of this study suggest that this actinomycete strain appears as an effective alternative in the remediation of lindane polluted sites.

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Isolation of four aquatic streptomycetes strains capable of growth on organochlorine pesticides

Cited by 97 publications

References 14 publications

Isolation of Brazilian marine fungi capable of growing on DDD pesticide

Isolation of Brazilian marine fungi capable of growing on DDD pesticide

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

Temperature and pH effect on lindane removal by Streptomyces sp. M7 in soil extract

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