Isolation of Fractions rich in Human Y Sperm

Rj, Ericsson; Cn, Langevin; Nishino, Masayuki

doi:10.1038/246421a0

Cited by 294 publications

(127 citation statements)

References 7 publications

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“…The present results confirmed previous reports of higher sperm velocities in X-sperm enriched fractions [14]. However, it should be noted that the faster swimming fraction of washed sperm is variable and depends on the type of sperm separation method, culture media and flow conditions [3,4,7,8,12,15]. It has been reported that testosterone in seminal plasma decreased sperm motility and a dose-dependent effect has been observed [13].…”

Section: Discussionsupporting

confidence: 81%

“…The increase in progressive motility as sperm moved down an albumin column has been observed in Y-sperm [4]. In contrast, differences in density, buoyancy or sedimentation velocity have been utilized to distinguish X-and Y-sperm in a multilayer Percoll preparation [8].…”

Section: Introductionmentioning

confidence: 99%

“…Controversial studies to distinguish X-and Y-bearing sperm have been reported [4,8]. The increase in progressive motility as sperm moved down an albumin column has been observed in Y-sperm [4].…”

Section: Introductionmentioning

confidence: 99%

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Sperm Velocity in Seminal Plasma and the Association With Gender of Offspring

Balli

Patton

Jacobson

et al. 2004

Archives of Andrology

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The gender of the offspring is determined by the fertilizing sperm. Previous gender studies were based on washed sperm, but not on sperm in seminal plasma. The objective was to correlate motility parameters assessed during semen analyses with the offspring gender. For comparison, fixed sperm head DNA quantitated by Hoechst 33342 fluorescence microscopy was also analyzed. Forty-six patients undergoing assisted reproduction procedures resulted in livebirth deliveries with either male or female-predominant offsprings. Sperm head fluorescence was weakly correlated to the gender in 61% of the cases. Sperm of patients with male offsprings had slower curvilinear (44.2 AE 1.8 mean AE SEM, versus, 49.9 AE 2.7 m=sec) and slower average path velocities (32.4 AE 1.2 versus 36.3 AE 1.7 m=sec). Using cut-off values for the curvilinear ( < 49 m=sec) and average path ( < 36 m=sec) velocities of sperm swimming in seminal plasma, the two parameters predicted 75 and 68% of the male offspring births, respectively. The data suggest that sperm movement in seminal plasma is a marker for factors that skew the ratio of the X-to Y-sperm populations.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

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Sperm Velocity in Seminal Plasma and the Association With Gender of Offspring

Balli

Patton

Jacobson

et al. 2004

Archives of Andrology

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“…A Cochrane systematic review of the two most common sperm preparation techniques has concluded that there is no difference in pregnancy rates after using swim-up or density gradient preparation (43). However, density gradient, which is the predominant method in the cohort of patients in our study, can lead to enrichment of Y-bearing spermatozoa (45)(46)(47), which could contribute towards increased sex ratios under IVF and IUI. IVF and IUI are closer to natural conception as the sperm compete for fertilization, whereas in ICSI the fertilizing spermatozoon is selected by the embryologist.…”

Section: Discussionmentioning

confidence: 99%

Effects of assisted reproductive technologies on human sex ratio at birth

Maalouf

Mincheva

Campbell

et al. 2014

Fertility and Sterility

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“…These apparent taxes, 'rheotaxis', 'thigmotaxis' and 'geotaxis', respectively, have all been studied separately. Two have become bases for explaining apparent success in techniques for separating X-and Y-chromosome bearing spermatozoa (Ericsson, Langevin & Nishino, 1973; Bhattacharya, Shome & Günther, 1977;Sarkar, 1981). Only Roberts (1970) has investigated the interaction of stimuli (flow and gravity) but he used 200-300 µ bore tubes which were observed in horizontal orientation only so there was little room for the 50 µ long bull spermatozoa used to develop a geotaxic response.…”

Section: Introductionmentioning

confidence: 99%