Isolation of Fungal Pathogens to an Edible Mushroom, <i>Pleurotus eryngii</i>, and Development of Specific ITS Primers

Kim, Sang Woo; Kim, Sinil; Lee, Hyun Jun; Park, Ju-Wan; Ro, Hyeon-Su

doi:10.5941/myco.2013.41.4.252

Cited by 11 publications

(4 citation statements)

References 13 publications

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“…The method is only suitable for fast-growing and nonfastidious microbes, and overgrown fungi capable of producing large amounts of spores suppressing other fungi [35]. Another perception in relation to the low microbial occurrence could be associated with antibacterial, antifungal agents, lytic enzymes and volatile compounds secreted by Pleurotus mushrooms and their flora microorganisms, which contribute to the survival strategies of the edible fungi by reducing the occurrence of spoilage microorganisms, since mushrooms are often confronted by different types of bacteria, fungi and viruses [36,37]. Hence, in associated ecosystem processes, a succession of microbial communities on wild mushrooms and their habitat (soil) involved intra-or interspecific interaction of different associations, which may include antibiosis, competition and mutualism [38].…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity, microbiological study and composition of soil associated with wild Pleurotus ostreatus from different locations in Ondo and Ekiti States, Nigeria

Familoni

Ogidi

Akinyele

et al. 2018

Chem. Biol. Technol. Agric.

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Background:Genetic variation among different strains of wild macrofungi can be expressed and documented using different molecular tools. In this study, the genetic diversity and relatedness of Pleurotus ostreatus from different locations in Ondo and Ekiti States were investigated. Methods:Random amplified polymorphic DNA-polymerase chain reaction marker was adopted to assess the genetic diversity of some wild P. ostreatus. Microorganisms associated with the wild P. ostreatus and their soil (substrate) were enumerated and identified using standard microbiological methods, while composition of soil around the mushrooms was evaluated using the conventional method.Results: A total of 114 positive DNA bands were observed among wild P. ostreatus based on random amplified polymorphic DNA analysis (RAPD) with 10 primers, showing a total number of polymorphic markers of 91 and average polymorphism of 80.24%. P. ostreatus from Ala quarters and Igbatoro Road in Akure related to P. ostreatus from AdoEkiti, while P. ostreatus from Ido-Ekiti and Usi-Ekiti are genetically similar. The bacterial count from wild P. ostreatus and associated soil ranged from 1.20 × 10 4 to 4.70 × 10 5 and 1.70 × 10 7 to 6.10 × 10 8 CFU g −1, respectively. The highest fungal count of 3.40 × 10 4 and 7.40 × 10 7 SFU g −1 were obtained for wild P. ostreatus and associated soil at Usi-Ekiti, which possesses the highest organic content (5.90%). Isolated microorganisms were Pseudomonas putida, Streptomyces spp., Trichoderma spp., Penicillium italicum and others. The highest crude fiber (25.04%) and protein content (24.07%) were obtained in wild P. ostreatus from Usi-Ekiti. Conclusion:RAPD revealed the genetic relatedness and genetic diversity among studied wild P. ostreatus, indigenous to two sates in Southwestern Nigeria. This will improve the strain selection for further utilization and documentation.

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Section: Resultsmentioning

confidence: 99%

Genetic diversity, microbiological study and composition of soil associated with wild Pleurotus ostreatus from different locations in Ondo and Ekiti States, Nigeria

Familoni

Ogidi

Akinyele

et al. 2018

Chem. Biol. Technol. Agric.

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“…The molecular markers TDP-F and TDP-R can detect the mycelium of Trichoderma not only independently (Figure 4) but also in a mixture of Trichoderma and edible mushrooms ( Figure 5). Although detection methods for T. harzianum, T. pleurotum, and T. pleuroticola have been developed in previous studies [10,29,30], the detection ranges of these previously developed methods are limited. Because the 11 Trichoderma spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

Lee¹,

Jung²,

Hong

et al. 2020

Mycobiology

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In Pleurotus sp., green mold, which is considered a major epidemic, is caused by several Trichoderma species. To develop a rapid molecular marker specific for Trichoderma spp. that potentially cause green mold, eleven Trichoderma species were collected from mushroom farms and the Korean Agricultural Culture Collection (KACC). A dominant fungal isolate from a green mold-infected substrate was identified as Trichoderma pleuroticola based on the sequences of its internal transcribed spacer (ITS) and translation elongation factor 1-a (tef1) genes. In artificial inoculation tests, all Trichoderma spp., including T. atroviride, T. cf. virens, T. citrinoviride, T. harzianum, T. koningii, T. longibrachiatum, T. pleurotum, and T. pleuroticola, showed pathogenicity to some extent, and the observed symptoms were soaked mycelia with a red-brown pigment and retarded mycelium regeneration. A molecular marker was developed for the rapid detection of wide range of Trichoderma spp. based on the DNA sequence alignment of the ITS1 and ITS2 regions of Trichoderma spp. The developed primer set detected only Trichoderma spp., and no cross reactivity with edible mushrooms was observed. The detection limits for the PCR assay of T. harzianum (KACC40558), T. pleurotum (KACC44537), and T. pleuroticola (CAF-TP3) were found to be 500, 50, and 5 fg, respectively, and the detection limit for the pathogen-to-host ratio was approximately 1:10,000 (wt/wt).

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“…Mushrooms on the other hand are reported to be suitable as substrates for different microorganisms [8]. Different fungi have also been reported to be isolated from decaying sawdust [9].…”

Section: Introductionmentioning

confidence: 99%

Abilities of Tectona grandis and Celtis zenkeri (Hardwood) Sawdust as Substrates of Pleurotus Species and Their Indigenous Fungi

Olatunji

Sobowale

Adenipekun

2019

JEAI

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Mushroom cultivation has continued to receive growing attention because of its nutritional and medicinal values. However, this study examined the effect of hardwood sawdust on the growth of Pleurotus ostreatus and Pleurotus pulmonarius were investigated. Relationship between fungal incidence of the substrates (sawdust) and that of the mushroom were examined. Both Pleurotus ostreatus and Pleurotus pulmonarius were inoculated on fermented and unfermented sawdust of Tectonal grandis and Celtis zenkeri. The fruiting bodies of the mushrooms were harvested and the growth parameters and biological efficiency was recorded. The isolated resident fungi were identified after obtaining pure cultures. The collected data were subjected to analysis (ANOVA) using Generalized Linear Model Procedure (GLM) of Statistical Analysis software (SAS). Means were separated using Duncan’s Multiple Range Test (DMRT) at p ≤ 0.05. Some of the growth parameters of P. ostreatus were significantly (p ≤ 0.05) better than that of P. pulmonarius. Tectona grandis and Celtis zenkeri sawdust had significant (p ≤ 0.05) impact on different growth parameters of the two mushrooms. Fermentation or non-fermentation of the substrates (sawdust) had no significant (p ≤ 0.05) impact on growth parameters of the mushrooms. Growth parameters of the two mushrooms were significantly better in 0% additive (p ≤ 0.05) than in the other additive concentrations. Five fungi were identified as indigenous fungi of the unfermented sawdust which did not significantly differ from those of the fermented sawdust and mushrooms. Nutritional composition of the mushrooms that grew on fermented and unfermented sawdust were good and comparable. The mushrooms were rich in protein, fibre, ash, moisture, fat and carbohydrate. Cultivation of mushrooms on hardwood sawdust is thus an effective means of managing such waste.

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Isolation of Fungal Pathogens to an Edible Mushroom, Pleurotus eryngii, and Development of Specific ITS Primers

Cited by 11 publications

References 13 publications

Genetic diversity, microbiological study and composition of soil associated with wild Pleurotus ostreatus from different locations in Ondo and Ekiti States, Nigeria

Genetic diversity, microbiological study and composition of soil associated with wild Pleurotus ostreatus from different locations in Ondo and Ekiti States, Nigeria

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

Abilities of Tectona grandis and Celtis zenkeri (Hardwood) Sawdust as Substrates of Pleurotus Species and Their Indigenous Fungi

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