Isolation of gametes from &lt;i&gt;Brachypodium distachyon&lt;/i&gt;

Matsumura, Takao; Okamoto, Takashi

doi:10.5511/plantbiotechnology.16.0123a

Cited by 4 publications

(3 citation statements)

References 52 publications

(49 reference statements)

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“…2e). The peripheral localization of vacuoles is consistent with the cellular characteristics of egg cells isolated from maize (Faure et al, 1992), wheat (Kovacs et al, 1994), rice (Uchiumi et al, 2007) and Brachypodium (Matsumura and Okamoto, 2016), suggesting that the cells isolated from the ovaries are egg cells.…”

Section: Isolation Of S Viridis Egg Cellssupporting

confidence: 75%

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Isolation of gametes and zygotes from Setaria viridis

et al. 2022

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Setaria viridis, the wild ancestor of foxtail millet (Setaria italica), is an effective model plant for larger C4 crops because it has several desirable traits, such as short generation time, prolific seed production and a small genome size. These advantages are well suited for investigating molecular mechanisms in angiosperms, especially C4 crop species. Here, we report a procedure for isolating gametes and zygotes from S. viridis flowers. To isolate egg cells, ovaries were harvested from unpollinated mature flowers and cut transversely, which allowed direct access to the embryo sac. Thereafter, an egg cell was released from the cut end of the basal portion of the dissected ovary. To isolate sperm cells, pollen grains released from anthers were immersed in a mannitol solution, resulting in pollen-grain bursting, which released sperm cells. Additionally, S. viridis zygotes were successfully isolated from freshly pollinated flowers. Isolated zygotes cultured in a liquid medium developed into globular-like embryos and cell masses. Thus, isolated S. viridis gametes, zygotes and embryos are attainable for detailed observations and investigations of fertilization and developmental events in angiosperms.

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Section: Isolation Of S Viridis Egg Cellssupporting

confidence: 75%

“…(40-50 µm; Uchiumi et al, 2006), but were similar to those of Brachypodium (30-35 µm; Matsumura and Okamoto, 2016). In addition, the isolated S. viridis egg cells showed many putative vacuoles, ranging in size from approximately 6 to 10 µm, that existed at their peripheral regions (Fig.…”

Section: Isolation Of S Viridis Egg Cellsmentioning

confidence: 72%

Isolation of gametes and zygotes from Setaria viridis

et al. 2022

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“…2e). The S. viridis egg cells were smaller than those of maize (60–77 μm; Kranz et al , 1991), wheat (50–70 μm; Kovacs et al , 1994) and rice (40–50 μm; Uchiumi et al , 2006), but were similar to those of Brachypodium (30–35 μm; Matsumura and Okamoto, 2016). In addition, the isolated S. viridis egg cells showed many putative vacuoles, ranging in size from approximately 6 to 10 μm, that existed at their peripheral regions (Fig.…”

Section: Resultsmentioning

confidence: 90%

Isolation of gametes and zygotes from Setaria viridis

Toda

Kiba

Kato

et al. 2021

Preprint

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SummarySetaria viridis, the wild ancestor of foxtail millet (Setaria italica), is an effective model plant for larger C4 crops because it has several desirable traits, such as short generation time, prolific seed production and a small genome size. These advantages are well suited for investigating molecular mechanisms in angiosperms, especially C4 crop species. Here, we report a procedure for isolating gametes and zygotes from S. viridis flowers. To isolate egg cells, ovaries were harvested from unpollinated mature flowers and cut transversely, which allowed direct access to the embryo sac. Thereafter, an egg cell was released from the cut end of the basal portion of the dissected ovary. To isolate sperm cells, pollen grains released from anthers were immersed in a mannitol solution, resulting in pollen-grain bursting, which released sperm cells. Additionally, S. viridis zygotes were successfully isolated from freshly pollinated flowers. Isolated zygotes cultured in a liquid medium developed into globular-like embryos and cell masses. Thus, isolated S. viridis gametes, zygotes and embryos are attainable for detailed observations and investigations of fertilization and developmental events in angiosperms.

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Wheat Cybrid Plants, OryzaWheat, Regenerated from Wheat–Rice Hybrid Zygotes via in Vitro Fertilization System Possess Wheat–Rice Hybrid Mitochondria

Maryenti,

Koshimizu,

Onda

et al. 2024

Plant and Cell Physiology

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Hybridization generates biodiversity, and wide hybridization plays a pivotal role in enhancing and broadening the useful attributes of crops. The hybridization barrier between wheat and rice, the two most important cereals, was recently overcome by in vitro production of allopolyploid wheat–rice hybrid zygotes, which can develop and grow into mature plants. In the study, genomic sequences and compositions of the possible hybrid plants were investigated through short- and long-read sequencing analyses and fluorescence in situ hybridization (FISH)-based visualization. The possible hybrid possessed whole wheat nuclear and cytoplasmic DNAs and rice mitochondrial (mt) DNA, along with variable retention rates of rice mtDNA ranging from 11% to 47%. The rice mtDNA retained in the wheat cybrid, termed Oryzawheat, can be transmitted across generations. In addition to mitochondrial hybridization, translocation of rice chromosome 1 into wheat chromosome 6A was detected in a F1 hybrid individual. OryzaWheat can provide a new horizon for utilizing inter-subfamily genetic resources among wheat and rice belonging to different subfamilies, Pooideae and Ehrhartoideae, respectively.

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Isolation of gametes from <i>Brachypodium distachyon</i>

Cited by 4 publications

References 52 publications

Isolation of gametes and zygotes from Setaria viridis

Isolation of gametes and zygotes from Setaria viridis

Isolation of gametes and zygotes from Setaria viridis

Wheat Cybrid Plants, OryzaWheat, Regenerated from Wheat–Rice Hybrid Zygotes via in Vitro Fertilization System Possess Wheat–Rice Hybrid Mitochondria

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