Isolation of genes encoding bovine IgM, IgG, IgA and IgE chains

Knight, K L; Becker, Robert S.

doi:10.1016/0165-2427(87)90123-1

Cited by 25 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with the Southern blot results reported by Knight et al (27), a short, ϳ280-bp S-like region, abundant in switch motifs, CTGGG (15 repeats) and CTGAG (12 repeats), was identified immediately upstream of the C␦ gene (Fig. 5).…”

Section: A Short S␦ Region Mediates Igd Class Switching In Cowsupporting

confidence: 79%

Artiodactyl IgD: The Missing Link

Zhao

Kacskovıcs

Pan

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

IgD has been suggested to be a recently developed Ig class, only present in rodents and primates. However, in this paper the cow, sheep, and pig Ig δ genes have been identified and shown to be transcriptionally active. The deduced amino acid sequences from their cDNAs show that artiodactyl IgD H chains are structurally similar to human IgD, where the cow, sheep, and pig IgD H chain constant regions all contain three domains and a hinge region, sharing homologies of 43.6, 44, and 46.8% with their human counterpart, respectively. According to a phylogenetic analysis, the Cδ gene appears to have been duplicated from the Cμ gene >300 million yr ago. The ruminant μCH1 exon and its upstream region was again duplicated before the speciation of the cow and sheep, ∼20 million yr ago, inserted upstream of the δ gene hinge regions, and later modified by gene conversion. A short Sδ (switch δ) sequence resulting from the second duplication, is located immediately upstream of the bovine Cδ gene and directs regular μ-δ class switch recombination in the cow. The presence of Cδ genes in artiodactyls, possibly in most mammals, suggests that IgD may have some as yet unknown biological properties, distinct from those of IgM, conferring a survival advantage.

show abstract

Section: A Short S␦ Region Mediates Igd Class Switching In Cowsupporting

confidence: 79%

Artiodactyl IgD: The Missing Link

Zhao

Kacskovıcs

Pan

et al. 2002

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…All heavy-chain isotype classes detected in other mammals were also described for cattle [17], [18], whereas the γ-isotype encompasses three sub-classes, namely γ1, γ2, and γ3 [18], [19]. The bovine IGH locus was assigned to the Bos taurus autosome (BTA) 21 [20] and localized on the q23-q24 bands [21] or on the q24 band respectively [22], [23].…”

Section: Introductionmentioning

confidence: 99%

Exceptionally Long CDR3H Are Not Isotype Restricted in Bovine Immunoglobulins

2013

View full text Add to dashboard Cite

Exceptionally long third complementarity determining regions of the heavy chain (CDR3H) were previously described as a specificity of bovine IgG and IgM immunoglobulins. In addition, the genomic organization of the immunoglobulin heavy chain locus remains to be elucidated with a special focus on the number of variable segments (IGHV). By analyzing the variable regions according to the isotype-specific PCR using cDNA-PCR, we were able to prove the existence of exceptional long CDR3H in all bovine isotypes. The corresponding sequences of three distinct amplicons were grouped according to the length of the CDR3H. Sequences of CDR3H possessed 5 to 10, 12 to 31 or at least 48 amino acid residues. Long and mid-length CDR3H were composed of mainly hydrophilic amino acid residues, while short CDR3H also contained hydrophobic amino acid residues. All sequences with long CDR3H were related to the germline variable segment 10. Using the current genome assembly, Bos taurus NCBI build 6.1, the genomic organization of the bovine immunoglobulin heavy-chain locus was analyzed. A main locus was investigated on BTA21. Exons coding for variable, diversity, and joining segments, as well as for the constant regions of different isotypes, were also localized on BTA7, BTA8, and BTA20. Together with the information from unplaced contigs, 36 IGHV were detected of which 13 are putatively functional. Phylogenetic analysis revealed two bovine IGHV families (boVH1, boVH2). Thus, the existence of the two bovine families suggested was demonstrated, where boVH1 comprises all functional segments. This study substantially improves the understanding of the generation of immunoglobulin diversity in cattle.

show abstract

“…Three subclasses of bovine IgG have been described previously (56,57), and two of these, IgG2 (58) and IgG3 (59), occur in two allelic forms. The half-life of the two major IgG isotypes (IgG1, IgG2) have been reported in several studies but the values are extremely divergent between publications (for a review, see Butler (56)).…”

Section: Bovine Igg Metabolismmentioning

confidence: 99%

Cloning and Characterization of the Bovine MHC Class I-Like Fc Receptor

Kacskovıcs

Simister

et al. 2000

The Journal of Immunology

View full text Add to dashboard Cite

In the cow, maternal immunity is exclusively mediated by colostral Igs, but the receptor responsible for the IgG transport has not yet been identified. The role of an IgG-Fc receptor (FcRn) that resembles a class I MHC Ag in transporting IgGs through epithelial cells was recently shown in selected species. We now report the cloning and characterization of the bovine FcRn (bFcRn). The cDNA and deduced amino acid sequences show high similarity to the FcRn in other species, and it consists of three extracellular domains, a hydrophobic transmembrane region, and a cytoplasmic tail. Despite the high similarity of the extracellular domains with other species, the bovine cytoplasmic tail is the shortest thus far analyzed. Aligning the known FcRn sequences, we noted that the bovine protein shows a 3-aa deletion compared to the rat and mouse sequences in the α1 loop. Furthermore, we found a shorter transcript of the bFcRn reflecting an exon 6-deleted mRNA, which results from an inadequate splice acceptor site in intron 5 and produces a transmembrane-deficient molecule, as was previously demonstrated in the related MHC class I gene family in mouse and humans. The presence of bFcRn transcripts in multiple tissues, including the mammary gland, suggests their involvement both in IgG catabolism and transcytosis.

show abstract

Isolation of genes encoding bovine IgM, IgG, IgA and IgE chains

Cited by 25 publications

References 12 publications

Artiodactyl IgD: The Missing Link

Artiodactyl IgD: The Missing Link

Exceptionally Long CDR3H Are Not Isotype Restricted in Bovine Immunoglobulins

Cloning and Characterization of the Bovine MHC Class I-Like Fc Receptor

Contact Info

Product

Resources

About