Isolation of genes expressed during the developmental stages of the oyster mushroom,Pleurotus ostreatus, using expressed sequence tags

Abstract

In order to isolate and identify the developmentally regulated genes during fruiting body development, cDNA libraries were constructed from eight developmental stages of the Oyster mushroom, Pleurotus ostreatus. From these libraries, 11 761 expressed sequence tags (PoESTs) were generated. Of these, 4060 different genes (PoUnigenes) were identified, representing 34.5% of the entire genome. Redundancy analysis of ESTs during the developmental stages identified eight, 13 and two genes that were specifically expressed in mycelia, fruiting body and basidiospore, respectively. RT-PCR was used to confirm the specific expression of nine genes which showed specific redundancy in fruiting body stages, four genes of which were expressed specifically in fruiting body stages as expected in redundancy analysis, and other genes were expressed abundantly in fruiting body stages. The EST database of P. ostreatus generated during this study provides a genetic and biochemical basis for future studies of the developmental stages of basidiomycetes.

Fungal aegerolysin-like proteins: distribution, activities, and applications

“…PlyA and Pe. ostreolysin (=EryA) (from Pleurotus eryngii), and two aegerolysins from the cocoa tree pathogen, Moniliophthora perniciosa, have all been shown to be present or highly expressed in primordia and fruiting bodies (Berne et al 2002;Fernandez Espinar and Labarere 1997;Joh et al 2007;Kurahashi et al 2014;Lee et al 2002;Vidic et al 2005). Time-course studies performed for the expression of Asphemolysin and terrelysin have also showed that their expression is highest during early fungal growth (Nayak et al 2012;Wartenberg et al 2011).…”

Hydrophobins: Proteins that self assemble at interfaces

“…Recent work has brought more understanding of these functions and new methods have been introduced for more systematic studies. In one study, expressed sequence tags were used to study gene expression during different developmental stages in the edible oyster mushroom, Pleurotus ostreatus [66]. In Beauveria bassiana, hydrophobins were pointed out to have key roles in adhesion and for cell surface properties [67].…”

Application of metatranscriptomics to soil environments

“…Exploratory multivariate statistical models can be used to identify differences in gene expression patterns between treatments, and along environmental gradients. Examples include: Between Group Analysis (Culhane et al, 2002), Redundancy Analysis (RDA; Joh et al, 2007), Canonical Correspondence Analysis (CCA; Liang et al, 2010), Permutational Multivariate Analysis of Variance (PERMANOVA; Anderson, 2001;Zapala and Schork, 2006), or Analysis of Similarities (ANOSIM; Huse et al, 2010). The advantage of RDA, CCA and BGA is that they allow the relationships between sites, species and treatments to be interpreted concomitantly.…”