Mycobacteria Protocols
DOI: 10.1385/0-89603-471-2:31
|View full text |Cite
|
Sign up to set email alerts
|

Isolation of Genomic DNA from Mycobacteria

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
107
0
1

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 129 publications
(109 citation statements)
references
References 15 publications
1
107
0
1
Order By: Relevance
“…Genomic DNA from the selected transposon mutants was extracted (Belisle & Sonnenberg, 1998), restriction digested with PvuII and analysed by Southern hybridization using a labelled fragment from the hygromycin-resistance cassette. The presence of a single hybridizing DNA fragment on the Southern blot verified that a single copy of the transposon had incorporated into the chromosome.…”
Section: Methodsmentioning
confidence: 99%
“…Genomic DNA from the selected transposon mutants was extracted (Belisle & Sonnenberg, 1998), restriction digested with PvuII and analysed by Southern hybridization using a labelled fragment from the hygromycin-resistance cassette. The presence of a single hybridizing DNA fragment on the Southern blot verified that a single copy of the transposon had incorporated into the chromosome.…”
Section: Methodsmentioning
confidence: 99%
“…Chromosomal DNA of the rifampinhypersensitive clone was isolated by standard methods (2). Total chromosomal DNA was cleaved with BamHI (MBI Fermentas), a restriction endonuclease that does not cut within the MycoMar element.…”
Section: Methodsmentioning
confidence: 99%
“…The PCR product from the deletion strain was sequence-verified. Genomic DNA was prepared according to the method of Belisle & Sonnenberg (1998). Southern hybridization was carried out using the AlkPhos Direct kit (Amersham) according to the manufacturer's instructions in order to confirm the expected genotype (Fig.…”
mentioning
confidence: 99%