Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities

Hasi, Rumana Yesmin; Majima, Dai; Morito, Katsuya; Ali, Hanif; Kogure, Kentaro; Nanjundan, Meera; Hayashi, Junji; Kawakami, Retsuo; Kanemaru, Kaori; Tanaka, Tamotsu

doi:10.1016/j.jchromb.2020.122213

Cited by 8 publications

(8 citation statements)

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“…Our recent report provided a method for the chemosynthesis of the major plant sphingolipid LCB t18:1, which contains both t18:1 8t and t18:1 8c conformations ( Liu et al., 2020a ), and purification procedures of either trans - or cis -t18:1 can be improved further. A troublesome problem is that plant GIPCs, always harvested after long extraction times, are a complex mixture with different ceramide backbones and head groups ( Buré et al., 2011 ; Hasi et al., 2020 ). Experiments using isolated GIPCs leave uncertainties because they rely mainly on the quality of the mixtures, and protocols to chemically synthesize the simple GIPCs have yet to be developed.…”

Section: Perspectivesmentioning

confidence: 99%

Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

Liu

Hou

Bao

et al. 2021

Plant Communications

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Sphingolipids, which comprise membrane systems together with other lipids, are ubiquitous in cellular organisms. They show a high degree of diversity across plant species and vary in their structures, properties, and functions. Benefiting from the development of lipidomic techniques, over 300 plant sphingolipids have been identified. Generally divided into free long-chain bases (LCBs), ceramides, glycosylceramides (GlcCers) and glycosyl inositol phosphoceramides (GIPCs), plant sphingolipids exhibit organized aggregation within lipid membranes to form raft domains with sterols. Accumulating evidence has revealed that sphingolipids obey certain trafficking and distribution rules and confer unique properties to membranes. Functional studies using sphingolipid biosynthetic mutants demonstrate that sphingolipids participate in plant developmental regulation, stimulus sensing, and stress responses. Here, we present an updated metabolism/degradation map and summarize the structures of plant sphingolipids, review recent progress in understanding the functions of sphingolipids in plant development and stress responses, and review sphingolipid distribution and trafficking in plant cells. We also highlight some important challenges and issues that we may face during the process of studying sphingolipids.

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Section: Perspectivesmentioning

confidence: 99%

Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

Liu

Hou

Bao

et al. 2021

Plant Communications

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“…The GlcCer, Cer, and HPS in the samples were separated, and their SB composition was analyzed using a slight modification of previous methods 20,24,25 . Briefly, crude lipids were extracted using a chloroform/methanol/water solvent Fig.…”

Section: Lipid Analysismentioning

confidence: 99%

“…, derivatized with o-phthalaldehyde OPA , and analyzed using a high-pressure liquid chromatography-fluorescence detector 28 . C1P and SB-1-phosphate SB1P were confirmed using TLC and Dittmer reagent which detects phosphorus 24 .…”

Section: Lipid Analysismentioning

confidence: 99%

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Sake (Rice Wine) Brewing Hydrolyzes Highly Polar Sphingolipids to Ceramides and Increases Free Sphingoid Bases

et al. 2021

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IntroductionComplex sphingolipids comprise a sphingoid base SB with an amide-linked fatty acid i.e., ceramide, Cer and a polar head group, such as phosphocholine or hexose 1, 2 . Plant and fungal sphingolipids contain neutral classes, mainly Cer and glucosylceramide GlcCer , and acidic classes, including inositol phosphoryl ceramide IPC and glycosyl IPC GIPC . In addition, di-, tri-, and tetra-glycosylceramides as neutral classes are also reported to exist in plants, including rice 3 .

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“…These compounds, as the inositols, are ubiquitous to eukaryotes and assume a strategic importance in living organisms being involved in growth, cellular signaling, stress responses etc. In plants and fungi, the main sphingolipds are glucosyl inositol phosphoryl ceramides (GIPCs) [30][31][32][33], which are not present in mammalian organisms [34] (Figure 4). A further class of biomolecules containing inositol moiety is represented by the so called archedityl inositols, a class of polar lipids isolated from extremophile Archean [35,36].…”

Section: Introduction 1inositolsmentioning

confidence: 99%

Novel Chemical and Biological Insights of Inositol Derivatives in Mediterranean Plants

2022

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Inositols (Ins) are natural compounds largely widespread in plants and animals. Bio-sinthetically they derive from sugars, possessing a molecular structure very similar to the simple sugars, and this aspect concurs to define them as primary metabolites, even though it is much more correct to place them at the boundary between primary and secondary metabolites. This dichotomy is well represented by the fact that as primary metabolites they are essential cellular components in the form of phospholipid derivatives, while as secondary metabolites they are involved in a plethora of signaling pathways playing an important role in the surviving of living organisms. myo-Inositol is the most important and widespread compound of this family, it derives directly from d-glucose, and all known inositols, including stereoisomers and derivatives, are the results of metabolic processes on this unique molecule. In this review, we report the new insights of these compounds and their derivatives concerning their occurrence in Nature with a particular emphasis on the plant of the Mediterranean area, as well as the new developments about their biological effectiveness.

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Isolation of glycosylinositol phosphoceramide and phytoceramide 1-phosphate in plants and their chemical stabilities

Cited by 8 publications

References 24 publications

Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives

Sake (Rice Wine) Brewing Hydrolyzes Highly Polar Sphingolipids to Ceramides and Increases Free Sphingoid Bases

Novel Chemical and Biological Insights of Inositol Derivatives in Mediterranean Plants

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