Isolation of Gonadotropin-Releasing Hormone II Receptor Within the Testis of the Pig.

Voss, Amy M.; Cederberg, Rebecca A.; Snyder, Matthew; Mills, G. A.; Kerl, Jill G.; White, Brett R.

doi:10.1093/biolreprod/85.s1.579

Cited by 2 publications

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“…In contrast, GnRH‐II predominantly immunolocalized to the tubular compartment of swine testes (Desaulniers et al, 2015a). Markedly, expression of GNRHR2 increased 76‐fold following puberty, implying an important role in the adult boar (Lents et al, 2017; Voss, 2013). These results suggest autocrine/paracrine regulation of Leydig cell function by GnRHR‐II signaling in the boar.…”

Section: Role Of Gnrh‐ii and Gnrhr‐ii In The Male Pigmentioning

confidence: 99%

Role of gonadotropin‐releasing hormone‐II and its receptor in swine reproduction

White

Cederberg

Elsken

et al. 2022

Molecular Reproduction Devel

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The pig represents the only livestock mammal capable of producing a functional protein for the second mammalian form of gonadotropin‐releasing hormone (GnRH‐II) and its receptor (GnRHR‐II). To examine the role of GnRH‐II and its receptor in pig reproduction, we produced a unique swine line with ubiquitous knockdown of endogenous GnRHR‐II levels (GnRHR‐II knockdown [KD]), which is largely the focus of this review. In mature GnRHR‐II KD males, circulating testosterone concentrations were 82% lower than littermate control boars, despite similar luteinizing hormone (LH) levels. In addition, nine other gonadal steroids were reduced in the serum of GnRHR‐II KD boars, whereas adrenal steroids (except 11‐deoxycortisol) did not differ between lines. Interestingly, testes from GnRHR‐II KD males had fewer, hypertrophic Leydig cells and fewer, enlarged seminiferous tubules than control testes. As expected, downstream reproductive traits such as androgen‐dependent organ weights and semen characteristics were also significantly reduced in GnRHR‐II KD versus control boars. Next, we explored the importance of this novel ligand/receptor complex in female reproduction. Transgenic gilts had fewer, but heavier, corpora lutea with smaller luteal cells than littermate control females. Although the number of antral follicles were similar between lines, the diameter of antral follicles tended to be larger in GnRHR‐II KD females. In regard to steroidogenesis, circulating concentrations of progesterone and 17β‐estradiol were lower in transgenic compared to control gilts, even though serum levels of follicle‐stimulating hormone and LH were similar. Thus, GnRH‐II and GnRHR‐II represent a potential avenue to enhance fertility and promote the profitability of pork producers.

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Section: Role Of Gnrh‐ii and Gnrhr‐ii In The Male Pigmentioning

confidence: 99%

Role of gonadotropin‐releasing hormone‐II and its receptor in swine reproduction

White

Cederberg

Elsken

et al. 2022

Molecular Reproduction Devel

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“…The abundance of GnRHR‐II mRNA increased 76‐fold in the testes of post‐pubertal compared to pre‐pubertal boars (Voss, ). Transcription of NPVF gene (RFRP precursor), NPFFR1 , and NPFFR2 in the boar testis decreased during this same period (Zheng et al, ).…”

Section: Gonadotropin‐releasing Hormone II (Gnrh‐ii) and Its Receptormentioning

confidence: 99%

RFamide‐related peptide 3 and gonadotropin‐releasing hormone‐II are autocrine–paracrine regulators of testicular function in the boar

Lents

Thorson

Desaulniers

et al. 2017

Molecular Reproduction Devel

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Widespread use of artificial insemination in swine requires millions of doses of boar semen each year. Subfertility of boars remains a major constraint, which can impact the reproductive efficiency of thousands of sows, so a better understanding of testicular function is needed in order to develop methods to improve semen production. With this in mind, the effects of RFamide-related peptide 3 (RFRP3) and Gonadotropin-releasing hormone-II (GnRH-II) on gonadotropin secretion and testicular function of pigs are reviewed here. Receptors for RFRP3 are present in the pig hypothalamus, adenohypophysis, and testis. Evidence from in vitro studies indicates that RFRP3 could be a hypophysiotropic hormone in the pig by suppressing secretion of GnRH-I from the hypothalamus and luteinizing hormone (LH) from the pituitary gland; however, effects of RFRP3 on in vivo secretion of LH in pigs are minimal. Within the pig testis, RFRP3 suppresses testosterone secretion by inhibiting steroidogenic enzymes. GnRH-II and its receptor (GnRHR-II) are abundant in pig testes. Interstitial cells express GnRHR-II, and exogenous GnRH-II robustly stimulates secretion of testosterone in boars, despite minimal secretion of LH. Data illustrate that GnRH-II directly stimulates secretion of testosterone from the testes of mature boars. Thus, the primary function of RFRP3 and GnRH-II in the boar appears to be autocrine-paracrine inhibition and stimulation, respectively, of testosterone secretion within the testis. A better understanding of changes in the RFRP3 and GnRH-II systems within the testis during development will provide important clues about how to improve the testicular function of boars.

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Isolation of Gonadotropin-Releasing Hormone II Receptor Within the Testis of the Pig.

Cited by 2 publications

References 0 publications

Role of gonadotropin‐releasing hormone‐II and its receptor in swine reproduction

Role of gonadotropin‐releasing hormone‐II and its receptor in swine reproduction

RFamide‐related peptide 3 and gonadotropin‐releasing hormone‐II are autocrine–paracrine regulators of testicular function in the boar

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