Isolation of high quality and yield of RNA from Agaricus bisporus with a simple, inexpensive and reliable method

Abstract

A method for isolating high purity and quantity RNA from Agaricus bisporus which is rich in proteins, carbohydrate, fiber and secondary metabolites, is described. RNA was extracted from mycelium, primordia, sporophores at two development stages and two post-harvest storage stages as well as from pileipellis, inner cap, gill and stipe of the mature sporophore. The A(260)/A(230) and A(260)/A(280) ratios of isolated RNA from fruiting bodies were both ~2 and the yield was about 200 μg/g fresh wt (FW). The yield of RNA from mycelium was approx. 100 μg/g FW. High quality RNA was also extracted from fruiting body tissues of Lentinus edodes, Pleurotus ostreatus, Flammulina velutipes and Pleurotus eryngii with yields from 130 to 225 μg/g FW. RNA extracted from all samples was intact, as demonstrated by gel electrophoresis and was suitable for downstream molecular applications, including RT-PCR and qPCR.

Effects of a high O2 dynamic-controlled atmosphere technology on the browning of postharvest white mushroom (Agaricus bisporus) in relation to energy metabolism

“…Total RNA was extracted by improved guanidine thiocyanate according to the method of Meng et al. (2012).…”

Postharvest treatment of arginine maintains the storage quality of fresh‐cut button mushrooms ( Agaricus bisporus )

“…Total RNA extraction of fresh‐cut button mushrooms was performed referring to Meng, Shen, et al’s (2012) method. According to the reference guide, contaminating genomic DNA was eliminated from the obtained RNA using RNase‐free DNase I (Promega).…”

“…The internal control was EF1‐α gene encoding an elongation factor (Meng et al, 2017). The nucleotide sequences of primers used for qPCR were the same as Meng, Shen, et al (2012) have reported. Data were obtained at 72°C.…”