Isolation of human factor VIII:C by preparative high-performance size-exclusion chromatography

Herring, Steven W.; Shitanishi, Kenneth T.; Moody, Katherine E.; Enns, Russel K.

doi:10.1016/s0021-9673(01)87447-1

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1998

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Cited by 6 publications

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“…In the last 2 decades a large variety of concen trates has been used starting with cryoprecipitate and sev eral kinds of intermediate and high purity preparations. Most recently, highly purified factor VIII concentrates obtained by utilizing immunoabsorbents have been pre pared [1][2][3][4][5][6][7][8][9]. The progress in the quality of factor VIII con centrates is summarized in table 1.…”

Section: Introductionmentioning

confidence: 99%

Factor VIII:c Concentrate Virus Inactivated: Progress in Purification by Using Classic Chromatographie Methods

Arrighi¹,

Pacenti²,

Borri³

1993

Vox Sanguinis

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This study was carried out with the aim of developing a production process for the manufacture of a highly purified factor VIII concentrate which is virus inactivated by pasteurization in liquid phase. Beside standard plasma protein separation techniques, the procedure uses a chromatographic step on anion exchanger, whose selectivity is increased by using high, but not destabilizing salt concentrations. The final product before stabilization has a specific activity higher than 300 IU/mg of protein, namely the highest specific activity reported for human factor VIII concentrates purified without the use of immunoabsorbents.

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