Isolation of Human Placental Fibroblasts

Ilić, Duško; Kapidzic, Mirhan; Genbačev, Olga

doi:10.1002/9780470151808.sc01c06s5

Cited by 15 publications

(14 citation statements)

References 37 publications

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“…Human umbilical vein cells (HUVECs) and smooth muscle cells were purchased from Lonza. As previously described, human placental fibroblasts (HPF) (45) and trophoblast progenitor cells (TBPCs) (46,47) were isolated and cultured. Human foreskin fibroblasts (HFF) were isolated and propagated by conventional methods (48).…”

Section: Cells and Virusesmentioning

confidence: 99%

A High-Affinity Native Human Antibody Neutralizes Human Cytomegalovirus Infection of Diverse Cell Types

Kauvar

Liu

Park

et al. 2015

Antimicrob Agents Chemother

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Section: Cells and Virusesmentioning

confidence: 99%

A High-Affinity Native Human Antibody Neutralizes Human Cytomegalovirus Infection of Diverse Cell Types

Kauvar

Liu

Park

et al. 2015

Antimicrob Agents Chemother

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“…With the aim of eliminating the xeno-contamination derived from MEFs, numerous studies have demonstrated the maintenance of hESCs on primary feeder cells from humans, such as adult skin fibroblasts [9] and placental fibroblasts [10]. Regardless of where the feeder cells are derived from, they are only viable for a few days; thus, the hESCs must be routinely transferred onto fresh feeder cells for continued culture.…”

Section: Introductionmentioning

confidence: 99%

A Synthetic Peptide-Acrylate Surface for Production of Insulin-Producing Cells from Human Embryonic Stem Cells

Lin

Hung

Yang

et al. 2014

Stem Cells and Development

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Human embryonic stem cells (hESCs), due to their self-renewal capacity and pluripotency, have become a potential source of transplantable b-cells for the treatment of diabetes. However, it is imperative that the derived cells fulfill the criteria for clinical treatment. In this study, we replaced common Matrigel with a synthetic peptide-acrylate surface (Synthemax) to expand undifferentiated hESCs and direct their differentiation in a defined and serum-free medium. We confirmed that the cells still expressed pluripotent markers, had the ability to differentiate into three germ layers, and maintained a normal karyotype after 10 passages of subculture. Next, we reported an efficient protocol for deriving nearly 86% definitive endoderm cells from hESCs under serumfree conditions. Moreover, we were able to obtain insulin-producing cells within 21 days following a simple three-step protocol. The results of immunocytochemical and quantitative gene expression analysis showed that the efficiency of induction was not significantly different between the Synthemax surface and the Matrigelcoated surface. Thus, we provided a totally defined condition from hESC culture to insulin-producing cell differentiation, and the derived cells could be a therapeutic resource for diabetic patients in the future.

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“…JEG-3 (choriocarcinoma cell line), J774 (mouse macrophage-like cell line), and MDCK (Madin-Darby canine kidney) cells were purchased from ATCC (https://www.atcc.org/) and propagated according to the instructions of ATCC. Human first-trimester placental fibroblasts and human trophoblast progenitor cells (hTPCs) were a generous gift from Susan Fisher and were propagated and differentiated as previously described (45,46). Mixed glial cell cultures were generated from newborn mice and plated onto poly-D-lysine (Millipore)-coated plates as previously described (47).…”

mentioning

confidence: 99%

InlP, a New Virulence Factor with Strong Placental Tropism

et al. 2016

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Intrauterine infection is a major detriment for maternal-child health and occurs despite local mechanisms that protect the maternal-fetal interface from a wide variety of pathogens. The bacterial pathogen Listeria monocytogenes causes spontaneous abortion, stillbirth, and preterm labor in humans and serves as a model for placental pathogenesis. Given the unique immunological environment of the maternal-fetal interface, we hypothesized that virulence determinants with placental tropism are required for infection of this tissue. We performed a genomic screen in pregnant guinea pigs that led to the identification of 201 listerial genes important for infection of the placenta but not maternal liver. Among these genes was lmrg1778 (lmo2470), here named inlP, predicted to encode a secreted protein that belongs to the internalin family. InlP is conserved in virulent L. monocytogenes strains but absent in Listeria species that are nonpathogenic for humans. The intracellular life cycle of L. monocytogenes deficient in inlP (⌬inlP) was not impaired. In guinea pigs and mice, InlP increased the placental bacterial burden by a factor of 3 log 10 while having only a minor role in other maternal organs. Furthermore, the ⌬inlP strain was attenuated in intracellular growth in primary human placental organ cultures and trophoblasts. InlP is a novel virulence factor for listeriosis with a strong tropism for the placenta. This virulence factor represents a tool for the development of new modalities to prevent and treat infection-related pregnancy complications.T he immunological environment of the maternal-fetal interface is unique because protection of the fetus from pathogens has to be balanced with tolerance of the fetus by the maternal immune system (1, 2). How this is accomplished is one of the major enigmas of mammalian reproduction. Contrary to the long-standing hypothesis that the pregnant mother is immunocompromised (3), recent evidence suggests that the maternal immune system is intricately regulated during pregnancy, and the placenta is well guarded against infection (4-6). A few predominantly intracellular microbes are able to infect the placenta and cause pregnancy complications such as preterm labor, fetal damage, and death (5, 7). Given the unique immunological environment of the maternal-fetal interface and the inability of many pathogens to colonize the placenta, we hypothesized that specific virulence determinants are required for microbes to survive and replicate in this tissue.Listeria monocytogenes is a facultative intracellular bacterial pathogen that causes spontaneous abortion, preterm labor, and stillbirth in humans and other mammals (8, 9). There are ϳ1,600 human cases in the United States per year, and about one-third of these cases are pregnancy associated (10). L. monocytogenes is also extremely amenable to experimental analysis and therefore has been exploited over the past 5 decades to understand host-pathogen interactions of intracellular microbes (11, 12). L. monocytogenes can infect a wide variety ...

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Isolation of Human Placental Fibroblasts

Cited by 15 publications

References 37 publications

A High-Affinity Native Human Antibody Neutralizes Human Cytomegalovirus Infection of Diverse Cell Types

A High-Affinity Native Human Antibody Neutralizes Human Cytomegalovirus Infection of Diverse Cell Types

A Synthetic Peptide-Acrylate Surface for Production of Insulin-Producing Cells from Human Embryonic Stem Cells

InlP, a New Virulence Factor with Strong Placental Tropism

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