Isolation of humic acid from the brown algaeAscophyllum nodosum, Fucus vesiculosus, Laminaria saccharina and the marine angiospermZostera marina

Radwan, Amal; Davies, Geoffrey; Fataftah, Amjad; Ghabbour, Elham A.; Jansen, Susan A.; Willey, Ronald J.

doi:10.1007/bf02186335

Cited by 30 publications

(17 citation statements)

References 8 publications

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“…Chemical analyses of extracts generally have focused on those components known to act as plant growth regulators such as auxins, betaines, cytokinins, and gibberellins, either in the commercial products or in the native seaweeds (Senn and Kingman 1978;Jameson 1993;Craft et al 2007;Hiltz et al 2007). The work of Patier et al (1993) on bioactive β-1,3-oligoglucans and humic acid in brown seaweeds by Radwan et al (1997) has broadened the work on bioactive compounds. Knowledge of the composition of the parent seaweed is useful but insufficient, since the use of alkali is common in the liquefaction process and can greatly increase the complexity of the resulting chemical matrix (Niemela and Sjostrom 1985).…”

Section: Discussionmentioning

confidence: 98%

Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

et al. 2008

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Three varieties of Kappaphycus alvarezii (Kapilaran, KAP), Tambalang purple (PUR), Adik-adik (AA), and one variety of Kappaphycus striatum var. sacol (green sacol (GS) were used to determine the efficiency of Acadian marine plant extract powder (AMPEP) as a culture medium at different concentrations, for the regeneration of young plants of Kappaphycus varieties, using tissue culture techniques for the production of seed stock for nursery and outplanting purposes for the commercial cultivation of carrageenophytes. A shorter duration for shoot formation was observed when the explant was treated with AMPEP+Plant Growth Regulator (PGR=PAA+zeatin at 1 mg L −1 ) compared to AMPEP when used singly. However, four explants responded differently to the number of days required for shoot formation. The KAP variety took 46 days to form shoots at 3-4 mg L −1 AMPEP+PGR; while PUR required 21 days at 3-5 mg L −1 AMPEP and 3-4 mg L −1 AMPEP+PGR. AA required 17 days at 3-5 mg L −1 AMPEP and AMPEP+PGR; and GS 25 days at 1 mg L −1 AMPEP+ PGR. It was observed that among the four explants used, PUR and AA initiated shoot formation with the use of AMPEP only at higher concentrations (3-5 mg L −1 ) after a shorter period. Only PUR responded positively to ESS/2 for shoot initiation. The use of AMPEP alone and/or in combination with PGR as a culture medium in the propagation of microplantlets using tissue culture technique is highly encouraging.

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Section: Discussionmentioning

confidence: 98%

Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

et al. 2008

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“…Nevertheless, due to the complexity of the subject and the absence of selectivity of the reagents used for humus isolation, there is no agreement among scientists as to wheter humus from compost effectively could assume the same meaning as in soil. For example, Radwan et al (1997) used the term HAs to indicate the alkali-soluble fraction isolated from algae. Therefore, often, a terminology such as HA-like material is used instead of HA to indicate the fraction isolated from compost (Filip et al 2000).…”

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confidence: 99%

Evolution of humic acid-like and core-humic acid-like during high-rate composting of pig faeces amended with wheat straw

Genevini¹,

Adani²,

Veeken

et al. 2002

Soil Science and Plant Nutrition

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“…Chemical analyses of extracts generally have focused on those components known to act as plant growth regulators such as auxins, betaines, cytokinins and gibberellins, either in the commercial products or in the native seaweeds (Senn and Kingman 1978;Jameson 1993;Craft et al 2007;Hiltz et al 2007). Recent interest in bioactive β-1,3-oligoglucans (Patier et al 1993) and a report that humic acids may occur in brown seaweeds (Radwan et al 1997) has broadened the focus of work. None of these studies permit the identification of a particular seaweed extract or allow for the quantitative determination of extract in a commercial product.…”

Section: Discussionmentioning

confidence: 98%

Liquid seaweed extracts identified using 1H NMR profiles

MacKinnon

Walter

2007

J Appl Phycol

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Aqueous extracts of Ascophyllum nodosum and several other brown seaweeds are manufactured commercially and widely distributed for use on agricultural crops. The increasingly regulated international trade in such products requires that they be standardized and defined to a degree not previously required. We examined commercially available extracts using quantitative 1 H NMR and principal components analysis (PCA) techniques. Extracts manufactured over a 4-year period using the same process exhibited characteristic profiles that, on PCA, clustered as a discrete group distinct from the other commercial products examined. In addition to recognizing extracts made from different seaweeds, analysis of the 1 H spectra in the 0.35-4.70 ppm region allowed us to distinguish amongst extracts produced from the same algal species by different manufacturers. This result established that the process used to make an extract is an important variable in defining its composition. A comparison of the 1 H NMR integrals for the regions 1.0-3.0 ppm and 3.0-4.38 ppm revealed small but significant changes in the A. nodosum spectra that we attribute to seasonal variation in gross composition of the harvested seaweed. Such changes are reflected in the PCA scores plots and contribute to the scatter observed within the data point cluster observed for Acadian soluble extracts when all data are pooled. Quantitative analysis using 1 H NMR (qNMR) with a certified external standard (caffeine) showed a linear relationship with extract concentration over at least an order of magnitude (2.5-33 mg/mL; R 2 >0.97) for both spectral regions integrated. We conclude that qNMR can be used to profile (or "fingerprint") commercial seaweed extracts and to quantify the amount of extract present relative to a suitably chosen standard.

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Isolation of humic acid from the brown algaeAscophyllum nodosum, Fucus vesiculosus, Laminaria saccharina and the marine angiospermZostera marina

Cited by 30 publications

References 8 publications

Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties

Evolution of humic acid-like and core-humic acid-like during high-rate composting of pig faeces amended with wheat straw

Liquid seaweed extracts identified using 1H NMR profiles

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