Isolation of <i>Brucella melitensis</i> from cattle in South Africa

Kolo, Francis Babaman; Fasina, Folorunso Oludayo; Ledwaba, Betty; Glover, Barbara; Dogonyaro, Banenat B.; Adesiyun, Abiodun A.; Katsande, T C; Matle, Itumeleng; Gelaw, Awoke K.; Heerden, Henriëtte van

doi:10.1136/vr.k2450

Cited by 13 publications

(11 citation statements)

References 2 publications

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“…abortus bv 1 has been documented to be the common species and biotype infecting the cattle population in South Africa (Coetzer et al., ; Gradwell, ) and in other countries especially in the Southern African region and the Caribbean (Fosgate et al., ; Matope, Bhebhe, Muma, Skjerve, & Djønne, ; Muendo et al., ). The isolation of B. melitensis from cattle in this study is however significant because it is considered the first documentation of B. melitensis in the cattle population in South Africa (Kolo et al., ). The occurrence of B. melitensis in cattle in this study may be as a result of rearing cattle together with sheep, goats and wildlife on the same farm or sharing of grazing land with sheep or goats (Radostits et al., ; Verger, Garin‐Bastuji, Grayon, & Mahé, ).…”

Section: Discussionmentioning

confidence: 80%

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Seroprevalence and characterization of Brucella species in cattle slaughtered at Gauteng abattoirs, South Africa

Kolo

Adesiyun

Fasina

et al. 2019

Veterinary Medicine & Sci

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BackgroundBrucellosis is an infectious and contagious zoonotic bacterial disease of both humans and animals. In developing countries where brucellosis is endemic, baseline data on the prevalence of brucellosis, using abattoir facilities, is important.ObjectivesThe aim of this study was to determine the seroprevalence of antibodies against Brucella in slaughter cattle at Gauteng province, South Africa and to characterize isolates of Brucella spp.MethodsIn this cross‐sectional study, un‐clotted blood samples with corresponding organ tissue samples were collected from slaughtered cattle. Serological [Rose Bengal test (RBT), complement fixation test (CFT) and indirect ELISA (iELISA)], molecular (PCR) and bacteriological methods were used to detect Brucella antibodies and Brucella spp. from 200 slaughtered cattle in 14 abattoirs.ResultsThe RBT revealed a seroprevalence of brucellosis as 11.0% (22 of 200) and iELISA confirmed 5.5% (11 of 200). The estimated seroprevalence from RBT and iELISA was 5.5% while RBT and CFT was 2.0% (4 of 200). Brucella melitensis (n = 6) and B. abortus (n = 5) were isolated from 11 cattle tissues (5.5%) as confirmed to species level with AMOS PCR and differentiated from vaccine strains with Bruce‐ladder PCR. Seven of the 11 isolates originated from seropositive cattle of which five were biotyped as B. abortus bv 1 (n = 2) and B. melitensis bv 2 (n = 1) and B. melitensis bv 3 (n = 2).ConclusionsThis is the first documentation of B. melitensis in cattle in South Africa. The zoonotic risk of brucellosis posed by Brucella‐infected slaughter cattle to abattoir workers and consumers of improperly cooked beef cannot be ignored.

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Section: Discussionmentioning

confidence: 80%

“…Africa (Kolo et al, 2018). The occurrence of B. melitensis in cattle in this study may be as a result of rearing cattle together with sheep, goats and wildlife on the same farm or sharing of grazing land with sheep or goats (Radostits et al, 2006;Verger, Garin-Bastuji, Grayon, & Mahé, 1989).…”

Section: Discussionmentioning

confidence: 87%

Seroprevalence and characterization of Brucella species in cattle slaughtered at Gauteng abattoirs, South Africa

Kolo

Adesiyun

Fasina

et al. 2019

Veterinary Medicine & Sci

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“…Infection was confirmed through culture and biotyping, with the causative organism identified as B. melitensis biovar 1 and 3 in Outbreak 1 and 2, while B. melitensis biovar 1 was identified in Outbreak 3 on Farm 1. Both have been previously been reported in South African livestock; B. melitensis biovar 2 and 3 were isolated from cattle [ 37 ] and B. melitensis biovar 1 from sheep [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

Investigation of Brucella melitensis in Sable Antelope (Hippotragus niger) in South Africa

et al. 2020

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In this study, Brucella melitensis infection in sable antelope (Hippotragus niger) was investigated on two wildlife ranches in South Africa over a 12-year period in order to determine the origin of the outbreaks and the role of livestock in maintaining the disease. Retrospective data were obtained from farm records and interviews as well as samples tested from different disease scenarios and clinical settings. On one ranch, 10 of 74 sable tested seropositive for B. melitensis in 2004 but were certified clear of infection after no further brucellosis cases were detected following repeated serological tests and culling over a five-year period. Recrudescence occurred in 2013 (7 of 187 brucellosis positives) and in 2014 (one positive), with persistent, latent infection being the most reasonable explanation. In a second case study, linked to the first one through a common vendor, 15 of 80 sable tested positive in 2016, some five years after the acquisition of the animals from a putative source. Brucella melitensis biovar 1 and/or 3 were isolated from each outbreak on both ranches. Both outbreaks resulted in substantial losses for the owners, arising from testing and culling and significant resource expenditure by the state. The study identified the diagnostic challenges for identifying and resolving disease outbreaks in wildlife, the persistence of B. melitensis in sable, the risks associated with animal movements, and the need for a wildlife-sensitive disease control scheme. Although the actual source of infection could not be identified, the investigation points away from local livestock as a source of ongoing infection while the persistent infection is consistent with the disease circulating within small, ranched populations and being spread through the keeping and trading of high-value animals. The implications of the study findings to disease control in wildlife are discussed.

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“…Even though attempts to isolate Brucella species circulating in the region were not successful, these results reveal more than one seroreactor animal species in villages and household visited. While the possibility of the transmission of Brucella species outside the ‘preferred’ host cannot be ruled out [ 56 – 58 ] particularly when animal species mix so freely, this may suggest that both B . abortus and B .…”

Section: Discussionmentioning

confidence: 99%

Brucellosis in ruminants and pastoralists in Borena, Southern Ethiopia

et al. 2020

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Brucellosis is a bacterial zoonotic disease that has important veterinary and public health consequences as well as economic impact in sub Saharan Africa including Ethiopia. A cross-sectional study was conducted in four selected districts of Borena Pastoral setting in Southern Ethiopia from October 2017 to February 2018 to estimate the prevalence of brucellosis and assess associated risk factors in cattle, sheep, goats and occupationally associated humans. A total of 750 cattle, 882 sheep and goats and 341 human subjects were screened for evidence of brucellosis using the Rose Bengal Test (RBT) with positive results confirmed by Competitive-ELISA(c-ELISA). Structured questionnaires were used for collection of metadata from individual animals, herders and animal attendants to test the association between explanatory and outcome variables. The overall animal level prevalence was 2.4% (95% confidence interval, CI: 1.4-3.7) in cattle, 3.2% (95% CI: 2.1-4.6) in sheep and goats, and 2.6% (95% CI: 1.2-5) in humans occupationally linked to livestock production systems. Herd size, parity, and history of abortion were risk factors associated with Brucella seropositivity (P<0.05) in cattle whereas in sheep and goats the results showed that district, age group, flock size, and history of abortion were significantly associated risk factors with Brucella seropositivity (P<0.05). Assisting calving and presence of seropositive animals in a household (P<0.05) were significantly associated with Brucella seropositivity in humans. Evidence of brucellosis in various animal species and the associated human population illustrates the need for a coordinated One Health approach to controlling brucellosis so as to improve public health and livestock productivity.

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Isolation of Brucella melitensis from cattle in South Africa

Cited by 13 publications

References 2 publications

Seroprevalence and characterization of Brucella species in cattle slaughtered at Gauteng abattoirs, South Africa

Seroprevalence and characterization of Brucella species in cattle slaughtered at Gauteng abattoirs, South Africa

Investigation of Brucella melitensis in Sable Antelope (Hippotragus niger) in South Africa

Brucellosis in ruminants and pastoralists in Borena, Southern Ethiopia

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