Isolation of <i>CarE</i> genes from the Chinese white pine beetle <i>Dendroctonus armandi</i> (Curculionidae: Scolytinae) and their response to host chemical defense

Dai, Lulu; Gao, Haiming; Ye, Jiaqi; Fu, Danyang; Sun, Yaya; Chen, Hui

doi:10.1002/ps.5205

Cited by 14 publications

(12 citation statements)

References 60 publications

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“…GST gene transcription was more sensitive in females to monoterpenes than that in males, but 3-carene was a special stimulus to males which caused extensive overexpression of GSTs. (+)-3-carene lead to higher male beetle mortality in fumigant toxicity tests than other terpenoids (Dai et al, 2015), and also had a greater impact on the transcription of most esterase genes (Dai et al, 2019). The higher expression levels of GSTs in females had a relationship with the resistance to host chemicals which might be also involved in female oviposition.…”

Section: Discussionmentioning

confidence: 99%

“…The transcriptional expression levels of D. armandi GSTs were normalized to the transcript levels of β-actin (KJ507199) and CYP4G55 (KR012821), as these two genes have stable expression levels in different stages, tissues, and feeding or terpenoids treatment (Dai et al, 2019). By calculating the geometric mean of these two genes, the accuracy of the relative expression level results can be improved.…”

Section: Real-time Qpcrmentioning

confidence: 99%

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Isolation, Expression Profiling, and Regulation via Host Allelochemicals of 16 Glutathione S-Transferases in the Chinese White Pine Beetle, Dendroctonus armandi

Gao

Dai

et al. 2020

Front. Physiol.

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Section: Discussionmentioning

confidence: 99%

Section: Real-time Qpcrmentioning

confidence: 99%

Isolation, Expression Profiling, and Regulation via Host Allelochemicals of 16 Glutathione S-Transferases in the Chinese White Pine Beetle, Dendroctonus armandi

Gao

Dai

et al. 2020

Front. Physiol.

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“…The specific expression patterns of EST genes in different developmental stages or sexes suggesting their possible functional roles (Campbell et al, 2003;Yang, 2016;. Some secreted ESTs (JHE and β-esterase) regulate development or reproduction via regulating the hormone and pheromones signal in insects (Dai et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

et al. 2020

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Esterases (ESTs) play important roles in metabolizing various physiologically endogenous and exogenous compounds, and various environmental xenobiotics in insects. The psocid, Liposcelis bostrychophila is a major pest of stored products worldwide and rapidly develops resistance to commonly insecticides. However, the involvement of ESTs in insecticide metabolization and the application of RNAi approach in psocids have not been well elucidated. In this study, we characterized four LbEST genes and investigated the transcriptional levels of these genes at different developmental stages and under different insecticides exposures to assess their potential roles in response to insecticides. The four LbESTs contain a catalytic triad (Ser-His-Glu) linked to an oxyanion hole and acyl pocket involved in substrate stabilization during its hydrolysis. Synergism observed with the esterase-inhibitor DEF suggests the involvement of esterases in malathion detoxification. LbESTs were expressed during the whole of developmental stages, but predominant abundance in the first nymphal instar and adult stage. The mRNA level of three LbEST genes (except for LbEST4) was induced (1.29-to 5.60 fold) in response to malathion or deltamethrin exposures, indicating that these esterases are involved in the detoxification process. Silencing of LbEST1, LbEST2 or LbEST3 through dsRNA feeding led to a higher mortality of psocids upon the malathion treatment compared to controls (1.83 to 2.69-fold), demonstrating that these esterase genes play roles in malathion detoxification in L. bostrychophila. Our study provides new evidence for understanding of the function and regulation mechanism of esterases in L. bostrychophila in insecticide detoxification. The current study also suggests that the present RNAi method could be applied for gene functional studies in psocids.

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“…Bark beetles have faced the toxic compounds of their host plants through different enzymes and molecular mechanisms (Robert et al, 2013; Dai et al, 2016, 2018; Chiu et al, 2019) of their own or from different symbiont microbes such as yeast, fungi, and bacteria. Some studies have shown that bark beetles-associated bacterial and filamentous fungi have developed different strategies and molecular mechanisms to excrete, metabolize into non-toxic compounds or sequester diverse terpenoid compounds (DiGuistini et al, 2011; Adams et al, 2013; Haridas et al, 2013; Lah et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data

et al. 2019

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Bark beetles from Dendroctonus genus promote ecological succession and nutrient cycling in coniferous forests. However, they can trigger outbreaks leading to important economic losses in the forest industry. Conifers have evolved resistance mechanisms that can be toxic to insects but at the same time, bark beetles are capable of overcoming tree barriers and colonize these habitats. In this sense, symbiont yeasts present in the gut of bark beetles have been suggested to play a role in the detoxification process of tree defensive chemicals. In the present study, genes related to this process were identified and their response to a terpene highly toxic to bark beetles and their symbionts was analyzed in the Cyberlindnera americana yeast. The genome and transcriptome of C. americana (ChDrAdgY46) isolated from the gut of Dendroctonus rhizophagus were presented. Genome analysis identified 5752 protein-coding genes and diverse gene families associated with the detoxification process. The most abundant belonged to the Aldo-Keto Reductase Superfamily, ATP-binding cassette Superfamily, and the Major Facilitator Superfamily transporters. The transcriptome analysis of non-α-pinene stimulated and α-pinene stimulated yeasts showed a significant expression of genes belonging to these families. The activities demonstrated by the genes identified as Aryl-alcohol dehydrogenase and ABC transporter under (+)-α-pinene suggest that they are responsible, that C. americana is a dominant symbiont that resists high amounts of monoterpenes inside the gut of bark beetles.

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Isolation of CarE genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense

Cited by 14 publications

References 60 publications

Isolation, Expression Profiling, and Regulation via Host Allelochemicals of 16 Glutathione S-Transferases in the Chinese White Pine Beetle, Dendroctonus armandi

Isolation, Expression Profiling, and Regulation via Host Allelochemicals of 16 Glutathione S-Transferases in the Chinese White Pine Beetle, Dendroctonus armandi

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

An Overview of Genes From Cyberlindnera americana, a Symbiont Yeast Isolated From the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae), Involved in the Detoxification Process Using Genome and Transcriptome Data

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