Isolation ofcsm1encoding a class V chitin synthase with a myosin motor-like domain from the rice blast fungus,Pyricularia oryzae

Park, In-Cheol; Horiuchi, Hiroyuki; Hwang, Cher Won; Yeh, Wan Hae; Ohta, Akio; Ryu, Jin Chang; Takagi, Masamichi

doi:10.1111/j.1574-6968.1999.tb13365.x

Cited by 31 publications

(3 citation statements)

References 14 publications

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“…P. oryzae , the causal agent of rice blast disease, is a filamentous heterothallic Ascomycete with septate hyphae that contains a single nucleus per cell. When P. oryzae infects rice plants, rice blast disease appears as brown spots in ears, leaves, and stems of rice plants and leads to severe damage to the rice plants, resulting in a distinctive reduction of the grain yield. − …”

Section: Resultsmentioning

confidence: 99%

“…When P. oryzae infects rice plants, rice blast disease appears as brown spots in ears, leaves, and stems of rice plants and leads to severe damage to the rice plants, resulting in a distinctive reduction of the grain yield. [41][42] Figure 2 shows the fungicidal activity of NOAC derivatives against P. oryzae at 0.5%. The results indicated that all of the synthesized compounds were more potent than the original chitosan at the same concentration.…”

Section: Fungicidal Activity Of Synthesized Compounds Against B Cinereamentioning

confidence: 99%

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Synthesis and Fungicidal Activity of New N,O-Acyl Chitosan Derivatives

et al. 2004

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Novel N,O-acyl chitosan (NOAC) derivatives were synthesized to examine their fungicidal activity against the gray mould fungus Botrytis cinerea (Leotiales: Sclerotiniaceae) and the rice leaf blast fungus Pyricularia oryzae (Teleomorph: Magnaporth grisea). The fungicidal activity was evaluated by the radial growth bioassay. NOAC derivatives were more active against the two plant pathogens than chitosan itself, and the effect was concentration dependent. Against B. cinerea, 4-chlorobutyryl chitosan (EC50=0.043%), decanoyl chitosan (EC50=0.044%), cinnamoyl chitosan (EC50=0.045%), and p-methoxybenzoyl chitosan (EC50=0.050%) were the most active (12-13-fold more active than chitosan). (Un)-substituted benzoyl chitosan derivatives were more active against B. cinerea than most of these with N,O-alkyl derivatives. Against P. oryzae chitosan derivatives with lauroyl, methoxy acetyl, methacryloyl and decanoyl were the most active.

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Section: Resultsmentioning

confidence: 99%

Section: Fungicidal Activity Of Synthesized Compounds Against B Cinereamentioning

confidence: 99%

Synthesis and Fungicidal Activity of New N,O-Acyl Chitosan Derivatives

et al. 2004

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“…The results of an InterProScan analysis (http://www.ebi. ac.uk/ InterProScan/) of the Lechs1 gene product (LeChs1) demonstrated that LeChs1 contains a myosin motor-like domain (MMD) (Fujiwara et al 1997) in the N-terminal region (comprising approximately 603 amino acids, amino acid residues 19 to 621) and a chitin synthase domain (CSD) (Fujiwara et al 1997) terminal MMD and a C-terminal CSD have been isolated and characterized from some fi lamentous, dimorphic and polymorphic fungi that produce true hyphae (Aufauvre-Brown et al 1997;Fujiwara et al 1997;Park et al 1999;Zhang et al 2000;Chigira et al 2002;Müller et al 2002;Epstein 2003, 2006;Madrid et al 2003;Niño-Vega et al 2004;Liu et al 2004;Takeshita et al 2006), and these chitin synthases have been grouped into class V or VI. Comparisons by Clustal W analysis of the deduced amino acid sequence of LeChs1 with those of previously classifi ed class V and class VI chitin synthases (Table 1) suggest the unique character of LeChs1, as mentioned below.…”

Section: Sequencing and Characterization Of Lechs1mentioning

confidence: 99%

Isolation and characterization of the gene encoding a chitin synthase with a myosin motor-like domain from the edible basidiomycetous mushroom, Lentinula edodes, and its expression in the course of fruit-body formation

2007

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We isolated and characterized the genomic and complementary DNAs encoding a chitin synthase from an edible basidiomycetous mushroom, Lentinula edodes. The gene (which we designated Lechs1) contains a large open reading frame encoding a polypeptide of 1937 amino acid residues. The open reading frame is interrupted by 14 small introns (49-116 bp). The gene product (LeChs1) consists of a myosin motor-like domain in its N-terminal half and a chitin synthase domain in its C-terminal half, analogous to the class V and VI chitin synthases of other fi lamentous fungi. Phylogenetic analysis demonstrated that LeChs1 is classifi ed into class VI chitin synthases. Southern blot analysis indicated that Lechs1 is a single-copy gene per haploid genome and that L. edodes has no other highly homologous chitin synthase genes. Northern blot analysis revealed that Lechs1 is expressed throughout the whole stages of fruitbody formation of L. edodes, but its expression level gradually declines in a fruit body-maturation-dependent manner with highest expression in vegetative mycelia and fruit body at the early stage of maturation (immature fruit body). This is the fi rst report on the isolation and characterization of the gene encoding a chitin synthase with a myosin motorlike domain from basidiomycetes.

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Insecticidal and fungicidal activity of new synthesized chitosan derivatives

Rabea

Badawy

Rogge

et al. 2005

Pest Management Science

144

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Chitosan, the N-deacetylated derivative of chitin, is a potential biopolysaccharide owing to its specific structure and properties. In this paper, we report on the synthesis of 24 new chitosan derivatives, N-alkyl chitosans (NAC) and N-benzyl chitosans (NBC), that are soluble in dilute aqueous acetic acid. The different derivatives were synthesized by reductive amination and analyzed by 1H NMR spectroscopy. A high degree of substitution (DS) was obtained with N-(butyl)chitosan (DS 0.36) at a 1:1 mole ratio for NAC derivatives and N-(2,4-dichlorobenzyl)chitosan (DS 0.52) for NBC derivatives. Their insecticidal and fungicidal activities were tested against larvae of the cotton leafworm Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae), the grey mould Botrytis cinerea Pers (Leotiales: Sclerotiniaceae) and the rice leaf blast Pyricularia grisea Cavara (Teleomorph: Magnaporthe grisea (Hebert) Barr). The oral feeding bioassay indicated that all the derivatives had significant insecticidal activity at 5 g kg(-1) in artificial diet. The most active was N-(2-chloro-6-fluorobenzyl)chitosan, which caused 100% mortality at 0.625 g kg(-1), with an estimated LC50 of 0.32 g kg(-1). Treated larvae ceased feeding after 2-3 days; the mechanism of action remains unknown. In a radial hyphal growth bioassay with both plant pathogens, all derivatives showed a higher fungicidal action than chitosan. N-Dodecylchitosan, N-(p-isopropylbenzyl)chitosan and N-(2,6-dichlorobenzyl)chitosan were the most active against B cinerea, with EC50 values of 0.57, 0.57 and 0.52 g litre(-1), respectively. Against P grisea, N-(m-nitrobenzyl)chitosan was the most active, with 77% inhibition at 5 g litre(-1). The effect of different substitutions is discussed in relation to insecticidal and fungicidal activity.

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Isolation ofcsm1encoding a class V chitin synthase with a myosin motor-like domain from the rice blast fungus,Pyricularia oryzae

Cited by 31 publications

References 14 publications

Synthesis and Fungicidal Activity of New N,O-Acyl Chitosan Derivatives

Synthesis and Fungicidal Activity of New N,O-Acyl Chitosan Derivatives

Isolation and characterization of the gene encoding a chitin synthase with a myosin motor-like domain from the edible basidiomycetous mushroom, <i>Lentinula edodes</i>, and its expression in the course of fruit-body formation

Insecticidal and fungicidal activity of new synthesized chitosan derivatives

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