Isolation of <i>Leclercia adcarboxglata</i> Strain JLS1 from Dolostone Sample and Characterization of its Induced Struvite Minerals

Han, Zuozhen; Sun, Bin; Zhao, Hui; Yan, Huaxiao; Han, Mei; Zhao, Yanyang; Meng, Ruirui; Zhuang, Dingxiang; Li, Dan; Ma, Yaoguang; Pei-yu, MA; Pan, Juntong

doi:10.1080/01490451.2016.1222469

Cited by 23 publications

(21 citation statements)

References 22 publications

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“…Homology comparison of the 16S rDNA sequence between strain ZW123 and other bacteria in the NCBI database was also conducted. The phylogenetic tree of the strain ZW123 was constructed using MEGA (Version 6, The Pennsylvania State University, Philadelphia, PA, USA) with the Neighbor-Joining method [22,32]. The physiological and biochemical identifications, such as Gram stain, Vopes-Prokauer (VP) test, H 2 S production, amylase test and bacterial motility test, and so on, were conducted according to previous studies [33].…”

Section: Isolation and Identification Of Bacterium Strain Zw123mentioning

confidence: 99%

“…In addition, the peaks at 700, 765, 872, 1064, 1401 and 1480 cm −1 record the presence of monohydrocalcite [7,58]. Characteristic peaks of struvite include the H-O-H group for water molecules at 1656 cm −1 ; the symmetrical and asymmetrical bending vibration of N-H (NH + 4 ) at 1609 and 1439 cm −1 ; the asymmetrical stretching vibration of PO 3− 4 at 1003.79 and 571.78 cm −1 ; the symmetrical bending vibration of PO 3− 4 at 453.16 cm −1 ; the hydrogen bond between waters at 762.4 cm −1 and the hydrogen bond between ammonium and water at 886.70 cm −1 [22,32]. As seen from Figure 7a Figure 7b) and these functional groups may be due to the amino acids and polysaccharides there.…”

Section: Ftir Analysesmentioning

confidence: 99%

“…Another strain of sulfate-reducing bacteria, LVform6, was isolated and used to induce dolomite under anaerobic conditions at low temperature [21]. Phosphate minerals, e.g., struvite, can also be induced by other sulfate-reducing bacteria [15,22]. Although many species of microorganisms have been investigated to induce carbonate and phosphate mineral precipitation, the bacterium Citrobacter freundii has rarely been employed; hence, in this study C. freundii was used as the experimental strain.…”

Section: Introductionmentioning

confidence: 99%

“…Braissant et al investigated the influence of exopolysaccharides and amino acids on the mineral phase and morphology and found that extracellular organic substances could act as nucleation sites [27]. Anaerobic metabolism for biomineralization has also been given special attention [21,22]. During the bacterial growth period, the pH changes of the medium, the concentrations of bicarbonate and carbonate ions, and the increasing sulfide content accompanied with decreasing sulfate due to anaerobic metabolism, have all been widely investigated [21].…”

Section: Introductionmentioning

confidence: 99%

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Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Sun

Zhao

et al. 2020

Minerals

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In this study, a facultative anaerobic strain isolated from marine sediments and identified as Citrobacter freundii, was used to induce the precipitation of carbonate and phosphate minerals in the laboratory under anaerobic conditions. This is the first time that the ability of C. freundii ZW123 to precipitate carbonate and phosphate minerals has been demonstrated. During the experiments, carbonic anhydrase, alkaline phosphatase and ammonium released by the bacteria not only promoted an increase in pH, but also drove the supersaturation and precipitation of carbonate and phosphate minerals. The predominant bio-mediated minerals precipitated at various Mg/Ca molar ratios were calcite, vaterite, Mg-rich calcite, monohydrocalcite and struvite. A preferred orientation towards struvite was observed. Scanning transmission electron microscopy (STEM) and elemental mapping showed the distribution of magnesium and calcium elements within Mg-rich calcite. Many organic functional groups, including C=O, C–O–C and C–O, were detected within the biominerals, and these functional groups were also identified in the associated extracellular polymeric substances (EPS). Fifteen kinds of amino acid were detected in the biotic minerals, almost identical to those of the EPS, indicating a close relationship between EPS and biominerals. Most amino acids are negatively charged and able to adsorb cations, providing an oversaturated microenvironment to facilitate mineral nucleation. The X-ray photoelectron spectroscopy (XPS) spectrum of struvite shows the presence of organic functional groups on the mineral surface, suggesting a role of the microorganism in struvite precipitation. The ZW123 bacteria provided carbon and nitrogen for the formation of the biotic minerals through their metabolism, which further emphasizes the close relationship between biominerals and the microorganisms. Thermal studies showed the enhanced thermal stability of biotic minerals, perhaps due to the participation of the bacteria ZW123. The presence of amino acids such as Asp and Glu may explain the high magnesium content of some calcites. Molecular dynamics simulations demonstrated that the morphological change and preferred orientation were likely caused by selective adsorption of EPS onto the various struvite crystal surfaces. Thus, this study shows the significant role played by C. freundii ZW123 in the bioprecipitation of carbonate and phosphate minerals and provides some insights into the processes involved.

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Section: Isolation and Identification Of Bacterium Strain Zw123mentioning

confidence: 99%

Section: Ftir Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Sun

Zhao

et al. 2020

Minerals

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“…The activity of CA and urease has been found in a wide range of microorganisms and plants [44]. CA and urease produced by bacterial metabolic activity also play an extremely important role in the biomineralization process [21,22,43,45] because they can increase the pH values in the culture medium, thus providing prerequisite conditions for the precipitation of minerals. In this work, CA and urease secreted by C. israelensis LD532 bacteria were also found.…”

Section: The Biological Activity Of Bacteria Affects Biomineralizationmentioning

confidence: 99%

Biomineralization of Carbonate Minerals Induced by the Halophilic <em>Chromohalobacter israelensis</em> under High Salt Concentrations: Implications for Natural Environments

Han¹,

Li²,

Zhao³

et al. 2017

Preprint

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Abstract:The mechanism underlying microbiologically induced carbonate precipitation have not been thoroughly characterized, although numerous scholars and experts have specifically investigated questions regarding minerals induced by bacteria. The study of the precipitation of carbonate minerals induced by halophilic bacteria has aroused wide concern. The present study aimed to investigate the characterization and process of biomineralization in high salt systems by a halophilic bacterium, Chromohalobacter israelensis strain LD532 (GenBank: KX766026), which was isolated from the Yinjiashan Saltern in China. Carbonate minerals induced by LD532 were investigated in several sets of comparative experiments that employed magnesium sulfate and magnesium chloride as Mg resources. Magnesium calcite and aragonite were induced by LD532 bacteria, whereas these minerals did not appear in the control group. The mineral phases, micromorphologies, and crystal structures were analysed using X-ray powder diffraction, scanning electron microscopy, and energy dispersive X-ray detection. The carbonic anhydrase and urease secreted by strain LD532 through metabolism increased the pH value of the liquid medium and promoted the process of carbonate precipitation. Further study using high resolution transmission electron microscopy, energy dispersive X-ray detection and analysis of ultrathin slices showed that the nucleation sites of carbonate minerals were located on extracellular polymeric substances and the membranes of intracellular vesicles of LD532 bacteria, which provided favourable conditions for the growth of carbonate mineral crystals. The morphologies and compositions of minerals formed in solutions of MgSO4 and MgCl2 display significant differences, indicating that different sources of Mg 2+ may also affect the physiological and biochemical activities of microorganisms and thus mineral deposition. This study will be of some interest for the interpretation of carbonate biomineralization in natural salt environments and has some value as a reference in understanding sedimentary carbonates in ancient marine environments, such as tidal flats.

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Struvite in circular economy: Production techniques, emerging applications and market opportunities

Chandrasekaran,

Zaffar,

Balasubramanian

2024

WIREs Energy & Environment

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Struvite precipitation is an efficient approach to recover phosphorus and ammonium from wastewater to alleviate many operational as well as environmental issues. The sparingly soluble nature of struvite in water makes it a potential slow‐release fertilizer whose adequacy has been investigated in various soil‐based cultivation. However, struvite has diverse applications other than field usage which are yet to be explored. The review highlights the usage of struvite as a fertilizer in soil along with its other applications as a nutrient supplier in hydroponic systems, fire retardants, and sorbent for the adsorption of heavy metals, dye, and gases. The research gaps between the lab and the application of the struvite products in the market for its proper utilization have also been reported. Thus, struvite produced from wastewater can be a promising green chemistry that could replace many costly, synthetic, and harmful products, while conserving phosphorous resource at the same time.This article is categorized under: Climate and Environment > Circular Economy Emerging Technologies > Materials Sustainable Development > Goals

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Isolation of Leclercia adcarboxglata Strain JLS1 from Dolostone Sample and Characterization of its Induced Struvite Minerals

Cited by 23 publications

References 22 publications

Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Bio-Precipitation of Carbonate and Phosphate Minerals Induced by the Bacterium Citrobacter freundii ZW123 in an Anaerobic Environment

Biomineralization of Carbonate Minerals Induced by the Halophilic <em>Chromohalobacter israelensis</em> under High Salt Concentrations: Implications for Natural Environments

Struvite in circular economy: Production techniques, emerging applications and market opportunities

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