Isolation of<i>Mycobacterium abscessus</i>from Japanese Medaka

Teska, Jeffrey D.; Twerdok, Lorraine E.; Beaman, Joseph R.; Curry, M. E.; Finch, Robert A.

doi:10.1577/1548-8667(1997)009<0234:iomafj>2.3.co;2

Cited by 24 publications

(16 citation statements)

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“…The selective medium, MMDA, formulated for enumeration of Mycobacterium abscessus (Teska et al 1997), was not effective for recovery of the majority of mycobacteria from striped bass. The ability of MMDA to support growth of various reference strains and field isolates varied within and among species.…”

Section: Discussionmentioning

confidence: 95%

“…Decontaminated homogenates (0.1 to 0.2 ml) from the initial group of samples (n = 44) were inoculated onto Lowenstein-Jensen (LJ) slants (BBL, Becton Dickson Diagnostic Systems) and Brain Heart Infusion agar (BHI) (Difco) plates containing 5% sheep red blood cells (BA), MDA and MDA modified to contain 0.025% malachite green, 0.04% cycloheximide and 0.0035% nalidixic acid (MMDA) (Teska et al 1997). Inoculated media were incubated at 30°C.…”

Section: Methodsmentioning

confidence: 99%

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Isolation and characterization of mycobacteria from striped bass Morone saxatilis from the Chesapeake Bay

Rhodes

Kator²,

Kaattari³

et al. 2004

Dis. Aquat. Org.

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Mycobacteriosis in striped bass Morone saxatilis of Chesapeake Bay, USA, was first diagnosed in 1997 based on the presence of granulomatous inflammation and acid-fast bacteria in skin and spleen. To confirm histopathology, bacteriological detection and identification of mycobacteria were begun using splenic tissue from fish with and without skin ulcerations. On the basis of initial studies using a variety of selective and nonselective media, decontamination, homogenization and incubation conditions, a simple and quantitative recovery method using aseptic necropsy of splenic tissue was developed. Optimal recovery was obtained by spread-plating homogenates on Middlebrook 7H10 agar with incubation for 3 mo at 23°C. Mycobacteria were recovered from 76% (n = 149/196) of fish examined. Mycobacterial densities exceeded 10 4 colony forming units · g tissue -1in 38% of samples (n = 63/168) that were examined using a quantitative approach. The most frequently recovered mycobacterium, present in 57% (n = 109/192) of characterized samples, was the recently named new species Mycobacterium shottsii. Polyinfections of M. shottsii and other mycobacteria were observed in 25% of samples (n = 47/192) with densities of M. shottsii usually 1 or more orders of magnitude higher than co-isolate(s). Other mycobacteria recovered included isolates that, based on phenotypic traits, resembled M. interjectum, M. marinum, M. scrofulaceum, M. szulgai and M. triplex. M. marinum, commonly associated with fish mycobacteriosis and human disease, was recovered infrequently (3%, n = 6/192). The presence of multiple mycobacterial types occurring at high densities suggests that a variety of mycobacteria could be causative agents of mycobacteriosis in striped bass from the Chesapeake Bay. Striped bass is the major recreational fish species in the Chesapeake Bay, and the significance of the current epizootic to human health and the potential adverse effects on fish stocks are not known. KEY WORDS: Mycobacteriosis · Fish disease · Striped bass · Morone saxatilis · Mycobacterium marinum · Mycobacterium shottsii Resale or republication not permitted without written consent of the publisherDis Aquat Org 61: [41][42][43][44][45][46][47][48][49][50][51] 2004 ded during the early to mid-1990s but by the late 1990s concern for the health of these fish returned. An epizootic in Chesapeake Bay striped bass, characterized by skin lesions and granulomatous inflammation with associated acid-fast bacteria, was first described in 1997 (Baya 1998, Vogelbein et al. 1998. Bacterial diseases in striped bass exhibiting skin lesions and scale loss have been attributed to a variety of aetiologies, including Aeromonas, Edwardsiella, Flexibacter, Pseudomonas, and Vibrio spp. (Toranzo et al. 1983, Mitchell 1984, Sindermann 1988, Plumb 1991, Baya et al. 1997. However, in the current epizootic the presence of nonbranching acid-fast bacteria within granulomas implicated mycobacteria as the causative agent and represented the first time that fish mycobacteriosis had ...

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Section: Discussionmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of mycobacteria from striped bass Morone saxatilis from the Chesapeake Bay

Rhodes

Kator²,

Kaattari³

et al. 2004

Dis. Aquat. Org.

View full text Add to dashboard Cite

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“…fortuitum and M. chelonae. M. avium, M. gordonae, M. abscesus, M. aurum, M. parafortuitum, M. poriferae, and M. triplex have also been isolated from fish (Arakawa and Fryer, 1984;Arakawa et al, 1986;Bragg et al, 1990;Dalsgaard et al, 1992;Tortoli et al, 1996;Teska et al, 1997;Bruno et al, 1998;Talaat et al, 1999;Diamant et al, 2000;Lehane and Rawlin, 2000;Herbst et al, 2001;Perez et al, 2001;Lescenko et al, 2003).…”

Section: Bacterial Pathogens Transmissiblementioning

confidence: 99%

Fish: a potential source of bacterial pathogens for human beings

Novotny¹,

Dvorská²,

Lorencova³

et al. 2004

Vet. Med.

208

187

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ABSTRACT:Human infections caused by pathogens transmi�ed from fish or the aquatic environment are quite common and depend on the season, patients' contact with fish and related environment, dietary habits and the immune system status of the exposed individual. They are o�en bacterial species facultatively pathogenic for both fish and human beings and may be isolated from fish without apparent symptoms of the disease. The infection source may be fish kept for both for food and as a hobby.

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“…The dramatic increase in the use of small fish species in research, particularly the zebrafish Danio rerio, has resulted in the recognition of fish mycobacteriosis as a concern to laboratory animal health management professionals (Astrofsky et al 2000, Sanders & Swaim 2001, Kent et al 2004, Matthews 2004. The most common bacterial species associated with fish mycobacteriosis include Mycobacterium chelonae, M. fortuitum, M. abscessus and, less frequently, M. marinum (Teska et al 1997, Chinabut 1999, Astrofsky et al 2000, Kent et al 2004. There is increasing evidence that while M. marinum is ubiquitous in freshwater and marine environments and can cause high mortality in captive fish, it does not appear to be common in zebrafish research facilities (Chinabut 1999, Astrofsky et al 2000, Kent et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Biochemical, molecular, and virulence characteristics of select Mycobacterium marinum isolates in hybrid striped bass Morone chrysops × M. saxatilis and zebrafish Danio rerio

Ostland

Watral²,

Whipps³

et al. 2008

Dis. Aquat. Org.

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A panel of 15 Mycobacterium marinum isolates was characterized by biochemical tests, sequencing the ribosomal DNA intergenic spacer (ITS) region and the heat shock protein 65 gene (hsp65) and pulsed-field gel electrophoresis (PFGE). The biochemical characteristics of all isolates were similar, except for Tween 80 hydrolysis. DNA sequence of hsp65 for a subset of isolates were identical; however, at position 5 of the ITS rDNA, a single nucleotide polymorphism was identified.Isolates possessing a guanine residue at this position (G strains) were unable to hydrolyze Tween 80, while isolates that contained an adenine residue at this position (A strains) were positive for Tween 80 hydrolysis. PFGE successfully discriminated between the G and A strains; all G strains had identical AseI restriction enzyme-cutting patterns while the A strains exhibited a variety of cutting patterns. Eight isolates (4 G and 4 A strains) were further characterized for virulence by experimental infection of hybrid striped bass (HSB) Morone chrysops × M. saxatilis and zebrafish Danio rerio. Seven of the 8 strains produced cumulative mortality ranging from 13.3 to 83.3% in the HSB virulence trial. The M. marinum reference strain ATCC 927 T did not produce mortality in HSB. HSB exposed to the G strains had significantly higher cumulative mortality than those exposed to the A strains. When these same isolates were tested in zebrafish, 6 of the 8 strains caused 100% cumulative mortality, with 2 of the A strains being the most pathogenic. In zebrafish, however, ATCC 927 T was virulent and produced 28.5% mortality. Collectively, we conclude that the M. marinum G strains are unique and may represent a distinct virulence phenotype in HSB, but this trend was not consistent in zebrafish. KEY WORDS: Mycobacterium marinum · Hybrid striped bass · Zebrafish · Mycobacteriosis · VirulenceResale or republication not permitted without written consent of the publisher

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Isolation ofMycobacterium abscessusfrom Japanese Medaka

Cited by 24 publications

References 0 publications

Isolation and characterization of mycobacteria from striped bass Morone saxatilis from the Chesapeake Bay

Isolation and characterization of mycobacteria from striped bass Morone saxatilis from the Chesapeake Bay

Fish: a potential source of bacterial pathogens for human beings

Biochemical, molecular, and virulence characteristics of select Mycobacterium marinum isolates in hybrid striped bass Morone chrysops × M. saxatilis and zebrafish Danio rerio

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