Isolation of<i>Theileria parasites</i>from African buffalo (<i>Syncerus caffer</i>) and characterization with anti-schizont monoclonal antibodies

Conrad, Patricia A.; Stagg, D.A.; Grootenhuis, J.G.; Irvin, A.D.; Newson, J.; Njamunggeh, R. E. G.; Rossiter, P. B.; Young, A. S.

doi:10.1017/s0031182000055761

Cited by 68 publications

(35 citation statements)

References 29 publications

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“…Similar reactions with anti-schizont MAbs were observed in some Zimbabwe Theileria isolates [13] and in some Theileria isolates from Kenyan buffalo which were found to consist of a number of distinctly different populations of Theileria parasites after cloning in vitro [4]. One of the major reasons for this change in character of the Southern stocks could be the characteristic carrier state of Theileria parasites in their mammalian hosts as described by Maritim et al [14].…”

Section: Discussionsupporting

confidence: 71%

Characterization of Some Theileria parva Stocks from Zambia Using Monoclonal Antibodies.

Nambota¹,

Lovelace²,

Chitambo³

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Theileria parva parasites have been isolated from different locations in Zambia where malignant theileriosis has been recorded. A total of 16 bovine lymphocytic cell lines infected with T. parva schizonts were characterized using a panel of anti-schizont monoclonal antibodies (MAbs). Comparison of the Theileria stocks isolated before (old) and after (new) the Muguga cocktail of T. parva from Kenya was used to vaccinate cattle against theileriosis in Zambia revealed differences in their reactivity against MAbs. The new isolates are showing MAb profiles similar to that exhibited by the Muguga cocktail which was used to vaccinate cattle in these areas between 1983 and 1989. These results suggest that the use of the Muguga cocktail to vaccinate animals against theileriosis in Zambia may have introduced Theileria stocks of different antigenic properties. -KEY WORDS: immunization, monoclonal antibody, Muguga cocktail, Theileria parva.

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Section: Discussionsupporting

confidence: 71%

Characterization of Some Theileria parva Stocks from Zambia Using Monoclonal Antibodies.

Nambota¹,

Lovelace²,

Chitambo³

et al. 1997

The Journal of Veterinary Medical Science

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“…While data regarding serological cross-reactivity are scarce, some strains of T. sp. (buffalo) might crossreact with T. parva antibodies and vice versa using IFAT or the PIM ELISA (Conrad et al 1987;AAL and BJM, unpublished observations).…”

Section: Discussionmentioning

confidence: 92%

Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation

et al. 2013

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Strict control measures apply to movement of buffalo in South Africa including testing for Theileria parva, the causative agent of Corridor disease in cattle. The official test is a real-time hybridization PCR assay that amplifies the 18S rRNA V4 hyper-variable region of T. parva, T. sp. (buffalo) and T. sp. (bougasvlei). Mixed infections with the latter organisms affect diagnostic sensitivity due to PCR suppression. While the incidence of mixed infections in the Corridor disease endemic region of South Africa is significant, little information is available on the specific distribution and prevalence of T. sp. (buffalo) and T. sp. (bougasvlei). Specific real-time PCR assays were developed and a total of 1211 samples known to harbour these parasites were screened. Both parasites are widely distributed in southern Africa and the incidence of mixed infections with T. parva within the endemic region is similar (* 25-50%). However, a significant discrepancy exists in regard to mixed infections of T. sp. (buffalo) and T. sp. (bougasvlei) (* 10%). Evidence for speciation between T. sp. (buffalo) and T. sp. (bougasvlei) is supported by phylogenetic analysis of the COI gene, and their designation as different species. This suggests mutual exclusion of parasites and the possibility of hybrid sterility in cases of mixed infections.

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“…Theileria sp. (buffalo) was first isolated from an infected buffalo in Kenya and then in South Africa (Allsopp et al, 1993;Conrad et al, 1987;Zweygarth et al, 2009). Very little is known about this parasite apart from the fact that it is very closely related to T. parva based on the 18S rRNA sequence (Allsopp et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a TaqMan real-time PCR for the detection of Theileria parva in buffalo and cattle

Papli

Landt

Fleischer

et al. 2011

Veterinary Parasitology

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Isolation ofTheileria parasitesfrom African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies

Cited by 68 publications

References 29 publications

Characterization of Some Theileria parva Stocks from Zambia Using Monoclonal Antibodies.

Characterization of Some Theileria parva Stocks from Zambia Using Monoclonal Antibodies.

Geographic distribution of Theileria sp. (buffalo) and Theileria sp. (bougasvlei) in Cape buffalo (Syncerus caffer) in southern Africa: implications for speciation

Evaluation of a TaqMan real-time PCR for the detection of Theileria parva in buffalo and cattle

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