Isolation of ice nucleating active bacteria from insects

Lee, Richard; Strong‐Gunderson, Janet M.; Lee, Marcia R.; Grove, Kimberly S.; Riga, Tammy J.

doi:10.1002/jez.1402570116

Cited by 62 publications

(30 citation statements)

References 20 publications

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“…However, while a plunge treatment such as this would kill any larvae on the outside, or near the surface of the fruit, larvae are often found deep within the fruit and may be protected against low temperatures due to thermal inertia in the fruit itself. Therefore, finding alternative methods of inoculative freezing, such as using ice‐nucleation active (INA) bacteria (Lee et al , 1991), to reduce cold hardiness in T. leucotreta may be a viable option. This method has been demonstrated with success in Coleoptera in the laboratory (Lee et al , 1994) and to a much lesser extent in Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

False codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae) larvae are chill‐susceptible

2011

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This study reports on the low temperature tolerance and cold hardiness of larvae of false codling moth, Thaumatotibia leucotreta. We found that larvae have mean critical thermal minima (lower limits of activity) of 6.7°C which was influenced by feeding status. The effects of low temperature exposure and duration of exposure on larval survival were assessed and showed that the temperature at which 50% of the population survives is −11.5 ± 0.3°C after 2 h exposure. The supercooling point (SCP, i.e., freezing temperature) was investigated using a range of cooling rates and under different conditions (feeding and hydration status) and using inoculative freezing treatments (in contact with water or orange juice). The SCP decreased significantly from −15.6°C to −17.4°C after larvae were fasted for 24 h. Twenty‐four hour treatments at either high or low relative humidity (95.9% or 2.4%) also significantly decreased SCP to −17.2°C and −18.2°C respectively. Inoculative freezing (by water contact) raised SCP from −15.6°C to −6.8°C which could have important implications for post‐harvest sterilization. Cooling rates did not affect SCP which suggests that there is limited phenotypic plasticity of SCP during the larval life‐stage, at least over the short time‐scales investigated here. In conclusion, larvae of T. leucotreta are chill‐susceptible and die upon freezing. These results are important in understanding this pest's response to temperature variation, understanding pest risk status and improving post‐harvest sterilization efficacy.

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Section: Discussionmentioning

confidence: 99%

False codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae) larvae are chill‐susceptible

2011

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“…Particles of food and bacteria in the guts of insects are thought to be potential ice nucleators (Lee et al 1991;Worland and Lukešová, 2000) but can be removed by cessation of feeding at low temperatures, although there is no evidence for deliberate ejection of gut contents in response to low temperatures. Recently, the effect of moulting on supercooling ability has been studied in Collembola (Worland, 2005;Worland et al 2006).…”

Section: Ice Nucleatorsmentioning

confidence: 99%

How insects survive the cold: molecular mechanisms—a review

2008

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Insects vary considerably in their ability to survive low temperatures. The tractability of these organisms to experimentation has lead to considerable physiology-based work investigating both the variability between species and the actual mechanisms themselves. This has highlighted a range of strategies including freeze tolerance, freeze avoidance, protective dehydration and rapid cold hardening, which are often associated with the production of specific chemicals such as antifreezes and polyol cryoprotectants. But we are still far from identifying the critical elements behind overwintering success and how some species can regularly survive temperatures below -20°C. Molecular biology is the most recent tool to be added to the insect physiologist's armoury. With the public availability of the genome sequence of model insects such as Drosophila and the production of custom-made molecular resources, such as EST libraries and microarrays, we are now in a position to start dissecting the molecular mechanisms behind some of these well-characterised physiological responses. This review aims to provide a state of the art snapshot of the molecular work currently being conducted into insect cold tolerance and the very interesting preliminary results from such studies, which provide great promise for the future.

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“…On the other hand, low-temperature stress would reduce the respiratory rate, inhibit aerobic respiration and damage the cell membrane system of insects, and cause membrane lipid peroxidation (Sargis & Subbaiah, 2006). By promoting fat metabolism and the tricarboxylic acid cycle (TCA cycle), these insects would not only generate energy, but also synthesize small molecules, such as sugars, alcohol and amino acids, which proliferate the content of bound water in the body, allowing these insects to resist cold environments, or directly interact with enzymes and other proteins to protect their life system (Lee, Gunderson, Lee, Grove, & Riga, 1991;Zhu et al, 2015Zhu et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of Locusta migratoria tibetensis Chen (Orthoptera: Oedipodidae) under high‐ and low‐temperature stress

Ran

Jiang

Qunfang

et al. 2020

J Applied Entomology

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The endemic locust species on the Tibetan plateau, Locusta migratoria tibetensis Chen can grow and breed successfully under the plateau temperature environment. This study explored the molecular response process of Tibetan locusts to high and low temperatures by comparing its transcriptome under different temperature conditions. A total of 66,256 unigenes were obtained through RNA‐Seq, among which 30,051 were successfully annotated. In detail, there were 778 differentially expressed genes (DEGs) at high temperatures and 1,787 DEGs at low temperatures. Under high temperatures, the metabolic pathways of saturated and unsaturated fatty acids and amino acids were up‐regulated, producing a significant amount of fatty acids and amino acids. However, under low temperatures, the chitinase activity was enhanced, causing chitin to decompose into small sugar molecules. Meanwhile, various sugar metabolism pathways were up‐regulated when a significant amount of small sugar molecules were produced. Then, large quantities of alpha‐linolenic acid and arachidonic acid were accumulated to maintain the normal physiological functions of cells. The analysis of annotated genes revealed that heat shock proteins (Hsp) were associated with the response to temperature stress, and mainly involved in the synthesis and transportation of proteins, preventing protein damage and the decomposition of damaged proteins. These results provide a basis for the further understanding of the adaptation mechanism of Tibetan locusts to high and low temperatures, laying a foundation for further understanding the adaptability of Tibetan locusts on Tibetan plateaus.

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Isolation of ice nucleating active bacteria from insects

Cited by 62 publications

References 20 publications

False codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae) larvae are chill‐susceptible

False codling moth Thaumatotibia leucotreta (Lepidoptera, Tortricidae) larvae are chill‐susceptible

How insects survive the cold: molecular mechanisms—a review

Comparative transcriptome analysis of Locusta migratoria tibetensis Chen (Orthoptera: Oedipodidae) under high‐ and low‐temperature stress

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