Isolation of infectious bronchitis virus from a flock of racing pigeons

Barr, D A; Reece, Richard L.; O’Rourke, Denise; Button, Chris; Faragher, J. T.

doi:10.1111/j.1751-0813.1988.tb14468.x

Cited by 21 publications

(24 citation statements)

References 2 publications

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“…We were also able to propagate viable coronaviruses from the RT-PCR-positive samples by passage in chick embryos. Although coronaviruses from chickens, turkeys, pheasants, pigeons and guineafowl can be propagated in chick embryos (Lister et al, 1985;Barr et al, 1988;Ito et al, 1991;Gough et al, 1996), there can be differences in yields between isolates. For example, the turkey coronaviruses replicate to about 10 4 -fold higher titres in turkey embryos than in chick embryos (Adams & Hofstad, 1971).…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of antigenicity and partial sequence data from isolated viruses, there are at least two additional avian species (pigeons and guineafowl) that are susceptible to IBV-like coronaviruses (Barr et al, 1988;Ito et al, 1991). From both practical and academic viewpoints, it is important to understand the extent to which an avian coronavirus from one species can replicate in another.…”

Section: Introductionmentioning

confidence: 99%

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Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Liu

Chen

et al. 2005

Journal of General Virology

132

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Coronavirus-like viruses, designated peafowl/China/LKQ3/2003 (pf/CH/LKQ3/03) and teal/China/LDT3/2003 (tl/CH/LDT3/03), were isolated from a peafowl and a teal during virological surveillance in Guangdong province, China. Partial genomic sequence analysis showed that these isolates had the S-3-M-5-N gene order that is typical of avian coronaviruses. The spike, membrane and nucleocapsid protein genes of pf/CH/LKQ3/03 had >99 % identity to those of the avian infectious bronchitis coronavirus H120 vaccine strain (Massachusetts serotype) and other Massachusetts serotype isolates. Furthermore, when pf/CH/LKQ3/03 was inoculated experimentally into chickens (specific-pathogen-free), no disease signs were apparent. tl/CH/LDT3/03 had a spike protein gene with 95 % identity to that of a Chinese infectious bronchitis virus (IBV) isolate, although more extensive sequencing revealed the possibility that this strain may have undergone recombination. When inoculated into chickens, tl/CH/LDT3/03 resulted in the death of birds from nephritis. Taken together, this information suggests that pf/CH/LKQ3/03 might be a revertant, attenuated vaccine IBV strain, whereas tl/CH/LDT3/03 is a nephropathogenic field IBV strain, generated through recombination. The replication and non-pathogenic nature of IBV in domestic peafowl and teal under field conditions raises questions as to the role of these hosts as carriers of IBV and the potential that they may have to transmit virus to susceptible chicken populations. INTRODUCTIONCoronaviruses (family Coronaviridae) belong to the order Nidovirales and contain a positive-stranded RNA genome that ranges from 27 to 31 kb in size (Cavanagh, 1997). Members of the family Coronaviridae infect a wide range of hosts and have been classified into three groups on the basis of antigenicity, genome organization and sequence similarity. Usually, coronaviruses infect only their normal target host species. It has, however, been reported that some strains of canine coronavirus and human coronavirus 229E can infect other, non-target species without causing disease (Barlough et al., 1984(Barlough et al., , 1985. The recent emergence of severe acute respiratory syndrome coronavirus (SARSCoV), which has been classified tentatively into group 2, has focused a great deal of interest on this virus family (Holmes, 2003). It was reported that SARS-CoV-like viruses were isolated from Himalayan palm civets (Guan et al., 2003) and ferrets (Mustela furo). Moreover, domestic cats (Felis domesticus) are susceptible to infection by SARSCoV, suggesting that the reservoir for this pathogen might involve a range of animal species (Martina et al., 2003).Infectious bronchitis virus (IBV), together with genetically related coronaviruses of turkey and pheasant, belongs to the group 3 coronaviruses. IBV is a pleomorphic, enveloped virus with club-shaped surface projections (spikes) and a single-stranded, positive-sense RNA genome of >27 kb in length (Boursnell et al., 1987). Upon virus entry into cells, a 39-coterminal nested set of six ...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Liu

Chen

et al. 2005

Journal of General Virology

132

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“…In Australia, in 1988, a coronavirus was isolated from tracheal/cloacal swabs, using chicken embryos, from racing pigeons that were exhibiting ruffled feathers, dyspnoea and excessive mucus at the beak (Barr et al ., 1988). The virus caused changes in the embryos typical of IBV, and it was neutralized by serum against an Australian serotype of IBV.…”

Section: Group 3 Coronaviruses In Greylag Goose Mallard Duck and Pigeonmentioning

confidence: 99%

Coronaviruses in poultry and other birds

2005

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“…Fowl, turkey and other bird species can be infected by IBV (Jonassen et al 2005). Barr et al (1988) firstly proved infection of IBV in the carrier pigeon, and he also found that this strain and the vaccine strain which had been generally applied in that place belonged to the same serotype. Soon after, Cavanagh et al (2002) reported an infection by coronaviruses and the close genetic relationship between IBV and TCoV through the analysis of genetic structure.…”

Section: Discussionmentioning

confidence: 99%

Analysis of similarity of the SI gene in infectious bronchitis virus (IBV) isolates in Shanghai, China

et al. 2007

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Análisis de similaridad del gen S1 de aislados del virus de la bronquitis infecciosa (IBV) en Shanghai, China J G Zhu, H D Qian, Y L Zhang, X G Hua*, Z L WuSchool of Agriculture and Biology, Shanghai Jiaotong University, 2678 Qixin Road, Shanghai 201101, P. R. China RESUMENEl objetivo del estudio fue analizar la similaridad del gen S1 de 5 nuevas cepas virales del virus de la bronquitis infecciosa (IBV) aislado en Shangai, China, para lo cual se usaron un par de partidores específicos, los cuales fueron designados en base a las secuencias publicadas del gen S del IBV. Los 5 segmentos completos de cDNA del gen S1 fueron amplificados de las 5 cepas aisladas con RT-PCR. Se pudo confirmar que las secuencias de nucleótidos de genes S1 en las 5 cepas aisladas de gallinas y palomas tienen una longitud de 1626 bp y codifican 541 aminoácidos residuales. Al comparar con la secuencia de las cepas de referencia de IBV seleccionadas publicadas en GenBank, las 4 cepas de gallinas exhibieron 77,4%-82,9% de identidad en la secuencia del gen S1, con una similaridad deducida de la secuencia proteica de 74,7%-82,6%, mientras que la cepa de paloma exhibió un 79,3%-99,6% de similaridad en la secuencia del gen S1 con una identidad deducida de la secuencia proteica de 81,6%-99,6%. La secuencia del sitio de división de la proteína S1 de estos aislados contiene 5 aminoácidos básicos consecutivos, llamados Arg-Arg-Phe-Arg-Arg (RRFRR), similares a la mayoría de las cepas IBV. El análisis de los resultados indicó que el rango de variabilidad de la estructura del gen S1 es alto y corresponde al carácter biológico de IBV y, molecularmente, las cinco cepas aisladas en Shangai se relacionan cercanamente con las cepas IBV de referencia. Se concluyó que las cinco cepas aisladas pertenecen al IBV, aun cuando una fue aislada desde palomas.Palabras clave: virus, bronquitis infecciosa, RT-PCR, gen S1, variación.

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Isolation of infectious bronchitis virus from a flock of racing pigeons

Cited by 21 publications

References 2 publications

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Isolation of avian infectious bronchitis coronavirus from domestic peafowl (Pavo cristatus) and teal (Anas)

Coronaviruses in poultry and other birds

Analysis of similarity of the SI gene in infectious bronchitis virus (IBV) isolates in Shanghai, China

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