Isolation of isosativenetriol from endophytic fungus Cochliobolus spp. of Aerva lanata

Shoba, S.; Sasikumar, Kandasamy; Sathiavelu, Mythili

doi:10.3329/bjp.v13i1.34953

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…After removal of the solvent using a rotary evaporator, the thick paste of MeOH extract was obtained. The oleanolic acid was isolated from methanolic extract using modified solvent-solvent fractionation without using any sophisticated chromatographic techniques (Sasikumar, Ghosh, 2017;Shoba, Sasikumar, Sathiavelu, 2018). The methanol extract was then resuspended with Mili Q water and the aqueous insoluble extract was further washed with EtOAc.…”

Section: General Experimentsmentioning

confidence: 99%

Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line

Sasikumar

Dubey

Ghosh

2020

Braz. J. Pharm. Sci.

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Vitis vinifera (black raisin) is commonly used in traditional medicine for the treatment of various ailments.In the present study, anti-oxidative and anti-cancer efficacy of oleanolic acid from ethyl acetate fraction of black raisins was evaluated and oleanolic acid was isolated without using of any chromatographic techniques and subjected to spectral assessment using UV-Vis spectrophotometer, 1 H NMR, 13 C NMR, MS and FT-IR for structural confirmation. Antiproliferative efficacy of oleanolic acid against human colon adenocarcinoma HCT-116 cells was assessed using cell viability assay. The minimum inhibitory concentration (IC 50 ) was determined and found to be 40 µg/mL at 48h incubation. Furthermore, antioxidant property of oleanolic acid was analyzed using DPPH method (IC 50 is 61.5µg/mL) by compared to standard antioxidants ascorbic acid, gallic acid, pyrogallol and butylated hydroxytoluene. Hence, the present study aims to establish the use of oleanolic acid as a potential therapeutic agent against human colon cancer.

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Section: General Experimentsmentioning

confidence: 99%

Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line

Sasikumar

Dubey

Ghosh

2020

Braz. J. Pharm. Sci.

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“…and Alternaria alternata inhibiting the heat-induced albumin denaturation (87.88, 86.89, and 87.03 µg/mL), the red blood cells membrane stabilization (78.42, 77.61, and 77.98 µg/mL) and the proteinase activity (85.21, 84.09, and 79.17 µg/mL) were the more potent [77]. In accordance, Shoba and Sathiavelu [78] showed that among the three solvent extracts from endophytic fungus Cochliobolus sp. from Aerva lanata, the methanol extract was the most potent exhibiting 62.51% inhibition of the egg albumin denaturation.…”

Section: Anti-inflammatory Agents Produced By Endophyticmentioning

confidence: 58%

Anti-leishmanial and Anti-inflammatory Agents from Endophytes: A Review

Toghueo

2019

Nat. Prod. Bioprospect.

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Leishmaniases and chronic inflammatory diseases are the cause of millions of deaths in the world each year. The treatment of leishmaniasis is facing serious drawbacks particularly due to the limited number of effective medicines, the resistance, and the toxicity of available drugs. On the other hand, many drugs are used for the management of inflammatory disorders. However, the most commonly prescribed although efficient is highly toxic with multiples side effects. New leads compounds for the development of new anti-leishmanial and anti-inflammatory drugs are needed. Over the past decade, several studies on the potential of endophytes to produce bioactive metabolites have been reported. We are presenting in the present review the status of research from 2000 to 2019 on the anti-leishmanial and anti-inflammatory metabolites isolated from endophytes from diverse habitats. An emphasis was put on existing gaps in the literature to inspire and guide future investigations. We hope that this review will help accelerate the drug discovery against leishmaniases and inflammation-associated disorders.

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