Isolation of “keplin B” from cerebral lipids

Svennerholm, Lars; Thorin, Hans

doi:10.1016/0006-3002(60)90031-7

Cited by 45 publications

(15 citation statements)

References 6 publications

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“…It seems unlikely that a phospholipid containing acyl groups would survive saponification and methanolic hydrochloric acid, but attempts to prove this were foiled by the presence of large amounts of methyl esters of fatty acids as contaminants. It is possible that the lipid may be comparable with that recently described by Svennerholm & Thorin (1960).…”

Section: Acid Hydrolysis Of Sphingomyelinsupporting

confidence: 57%

“…Carter, Smith & Jones (1958) isolated an acidand alkali-stable phospholipid from egg yolk which was shown to consist largely of a phosphorylethanolamine derivative of batyl alcohol and they suggested that such a lipid may be present in brain tissue. Very recently Svennerholm & Thorin (1960) have obtained more definite evidence of an acidand alkali-stable 'kephalin B' from brain which yielded ethanolamine on strong acid hydrolysis and was comparable analytically with the product of Carter et al (1958). In the present work no apparent water-soluble ninhydrinpositive material apart from sphingosylphosphorylcholine was obtained on methanolic hydro-182 1961 chloric acid hydrolysis of the acid-and alkali-stable phosphoipids of brain, and more than one watersoluble phosphate ester was often obtained in addition to those derived from sphingomyelin.…”

Section: Discussionmentioning

confidence: 54%

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Studies on cerebral sphingomyelin

Ansell¹,

Spanner²

1961

Biochemical Journal

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Section: Acid Hydrolysis Of Sphingomyelinsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 54%

Studies on cerebral sphingomyelin

Ansell¹,

Spanner²

1961

Biochemical Journal

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“…The product would be expected t o be eluted after sphingomyelin from silicic acid columns (29). By a n a l o w t o lysolecithin, it would be expected t o move slightly off the origin in thin-layer chromatograms, with the solvent system used here (22).…”

Section: Discussionmentioning

confidence: 99%

The Isolation of Beef Sphingomyelins

Spencer¹,

Schaffrin²

1964

Can. J. Biochem.

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A method was developed for the quantitative isolation of tissue sphingomyelins.Total lipid extracts of tissues were subjected to selective hydrolysis to destroy ester phosphatides and plasmalogens. The water-soluble hydrolysis products were removed by solvent fractionation. The sphingomyelins were separated from other lipids on silicic acid columns.Sphingomyelins were prepared from beef heart, lung, kidney, adrenal, brain, liver, and adipose tissue. The sphingomyelins were analyzed by thin-layer chromatography, paper chromatography of hydrolysis products, and infrared spectroscopy. Fatty acids were analyzed by gas-liquid chromatography.Methods for preparation and analysis of sphingomyelins are discussed.

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“…The present results therefore show that the nonsphingomyelin phospholipids present in the alkaliand acid-stable phospholipid fraction are largely cyclic acetal plasmalogens formed as artifacts during the acid treatment. In certain tissues small amounts of glyceryl ether phospholipids would also be present in this fraction (Svennerholm & Thorin, 1960;Ansell & Spanner, 1961) and it is probable that other minor components will eventually be identified. SUMMARY 1.…”

Section: Discussionmentioning

confidence: 99%