Isolation of Kerstersia gyiorum from a Patient with Cholesteatomatous Chronic Otitis Media

Almuzara, Marisa; Barberis, Claudia; Traglia, Germán Matías; Ordoñez, Andrea Martinez; Famiglietti, Ángela; Ramírez, María Soledad; Vay, Carlos

doi:10.1128/jcm.02051-12

Cited by 23 publications

(40 citation statements)

References 13 publications

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“…The scale bar indicates a 0.2 % difference in nucleotide sequences Bostwick et al 2015;Coenye et al 2003;Deutscher et al 2014;Mwalutende et al 2014;Pence et al 2013) (Table 2), and were different from the K. similis isolate ( Table 2). As shown in Table 3, the antibiotic sensitivity of the K. gyiorum isolate in our study is consistent with previous reports (Almuzara et al 2012;Deutscher et al 2014;Mwalutende et al 2014;Pence et al 2013), except for several antimicrobial agents of the isolate C (Bostwick et al 2015). It is not surprising since different strains of the same bacterial species can have different antibiotic sensitivity.…”

Section: Discussionsupporting

confidence: 81%

“…This phenotype appears to be characteristic of K. gyiorum. The biochemical characteristics of our isolate matched with other K. gyiorum isolates (Almuzara et al 2012; Fig. 3 Phylogenetic tree based on gyrB gene sequences of Kerstersia gyiorum and K. similis.…”

Section: Discussionmentioning

confidence: 96%

“…This is the first case of K. gyiorum isolated from an animal. In previous studies, K. gyiorum was found in rare cases to cause infections in humans resulting in otitis media (Almuzara et al 2012;Mwalutende et al 2014), bacteremia (Bostwick et al 2015), a chronic tracheostomy (Deutscher et al 2014), chronic ear infections (Pence et al 2013) and a chronic leg wound (Pence et al 2013). The bacterium has also been isolated from human fecal material and sputum (Coenye et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…K. gyiorum forms white opaque colonies with spreading edge margins on a blood agar plate. Arrows show colonies of K. gyiorum A, B, C, D and E are Kerstersia gyiorum species from references (Pence et al 2013), (Mwalutende et al 2014), (Deutscher et al 2014), (Bostwick et al 2015) and (Almuzara et al 2012), respectively. F is Alcaligenes faecalis species from reference (Coenye et al 2003).…”

Section: Discussionmentioning

confidence: 99%

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Isolation of culturable aerobic bacteria and evidence of Kerstersia gyiorum from the blowhole of captive Yangtze finless porpoises

Wan

McLaughlin

Zhou

et al. 2016

Antonie van Leeuwenhoek

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Bacterial respiratory illnesses are problematic in aquatic mammals such as the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis; YFP), which is now at a critically endangered status. Yet little is known about the bacteria inhabiting the respiratory tract of YFPs. In this study, we preliminarily characterized the culturable aerobic bacteria from blow samples of captive YFPs. The bacterial diversity was assessed through cultivation by direct exhalation onto Columbia blood agar plates and identification of representative isolates through 16S rRNA gene sequence analysis. In total, eleven bacterial species belonging to four phyla Proteobacteria (71 %), Firmicutes (25 %), Bacteroidetes (3 %) and Actinobacteria (1 %) were identified. Most of these isolates were opportunistic pathogens found in respiratory illnesses in humans and animals. We also reported the first case of Kerstersia gyiorum isolated from an animal. This work provides a preliminary assessment of the bacteria present in the respiratory tract of captive YFPs, which will be an important first step in elucidating the roles of normal microbiota in maintaining respiratory health of YFPs. This study also points out the necessity of future long-term monitoring of blowhole microorganisms in the YFPs and making emergency preparedness plans for respiratory tract infections. These measures can aid in assessing the pathogenic risk of the critically endangered YFP populations.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Isolation of culturable aerobic bacteria and evidence of Kerstersia gyiorum from the blowhole of captive Yangtze finless porpoises

Wan

McLaughlin

Zhou

et al. 2016

Antonie van Leeuwenhoek

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“…The isolate tested as susceptible to cefepime (Յ4 g/ml), gentamicin (Յ2 g/ml), meropenem (Յ1 g/ml), piperacillin-tazobactam (Յ16 g/ml), and trimethoprim-sulfamethoxazole (Յ0.5 g/ml) and intermediate to ciprofloxacin (2 g/ml). The patient was treated with 500 mg ciprofloxacin orally, twice daily for 10 days, based on case reports demonstrating susceptibility of K. gyiorum isolates to ciprofloxacin (5,6). Subsequently, an Etest battery was performed on both isolates for comparison between the two institutions (7) ( Table 1).…”

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confidence: 99%

Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection

Pence¹,

Sharon

TeKippe³

et al. 2013

J Clin Microbiol

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c Kerstersia gyiorum is infrequently associated with human infection. We report the isolation of Kerstersia gyiorum from two patients: the first, a patient with chronic ear infections, and the second, a patient with a chronic leg wound. Both isolates were resistant to ciprofloxacin, which has not been previously reported. CASE REPORTSC ase 1. A 55-year-old man with a past medical history of chronic ear disease, alcoholism, and smoking (2 packs/day) was seen in the Barnes-Jewish Hospital otolaryngology clinic with a chief complaint of bilateral ear drainage. At the ages of 13 and 16, he had undergone canal wall-down mastoidectomies of the right and left ears, respectively. Since that time, he had reported some hearing loss and bilateral ear drainage. One month prior to his current encounter, the patient complained of increasing drainage from his left ear, which reportedly exhibited a reddish hue and an odor of "dead fish." At that time, the patient was prescribed 0.3% ciprofloxacin-0.1% dexamethasone otic solution (four drops, twice daily). At a follow-up visit 1 month later, he admitted to being only partially compliant with his prescribed regimen. During the same visit, the left mastoid cavity was suctioned and cleaned and a specimen was taken from the posterior pocket at the sinodural angle and submitted for aerobic bacterial culture. The patient was instructed to continue using ciprofloxacindexamethasone drops and expressed that he would make an effort to be more compliant.The direct Gram stain of the specimen submitted from the mastoid cavity showed no polymorphonuclear cells, moderate numbers of Gram-positive bacilli, and moderate numbers of Gram-negative bacilli. The culture grew abundant amounts of Corynebacterium amycolatum, as well as an abundant amount of a Gram-negative coccobacillus, which appeared in singles, pairs, and short chains on Gram stain (Fig. 1A). The isolate formed flat, opaque, gray colonies with spreading edges on blood (Fig. 1B) and chocolate agar, with a colony morphology somewhat resembling that of Alcaligenes spp. but lacking the characteristic "fruity" odor associated with this genus. On MacConkey agar, the isolate was non-lactose fermenting, but colonies had a slight lavender hue (Fig. 1C), which was especially evident when the colonies were picked up using a swab (Fig. 1D). The isolate was oxidase negative, spot indole negative, catalase positive, and nonmotile. An oxidation/fermentation (OF) glucose test was performed; the isolate was found to be a nonutilizer of glucose. Disks containing vancomycin and penicillin were added to subculture plates to obtain additional information about the isolate; there was no inhibition around the vancomycin disk, and a zone size of 16 mm was measured around the penicillin disk. A Vitek 2 Gram-negative identification (GNI) card (bioMérieux, Durham, NC) resulted in no identification. A RapID NF plus assay (Thermo Fisher Scientific, Lenexa, KS) was performed and gave a biocode of 010200, which resulted in an identification of Pseudomonas oryzihabi...

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Bordetella and Related Genera

Kilgore,

Coenye

2023

ClinMicroNow

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Bordetellae are catalase positive and oxidize amino acids, but no carbohydrates are fermented. Achromobacter species are Gram‐negative, nonsporulating, small bacilli. Alcaligenes species are Gram‐negative, strictly aerobic rods or coccobacilli that possess oxidase and catalase activity. Cells of Kerstersia and Advenella species are Gram‐negative, small, and rod‐shaped or coccoid and occur alone, in pairs, or in short chains. Motility is strain dependent. Cells from Paenalcaligenes species are Gram‐negative, small rods. Many Achromobacter species have been recovered from clinical samples or from the hospital environment. Direct fluorescent‐antibody staining (DFA) on nasopharyngeal swabs or aspirates to detect B. pertussis or B. parapertussis is rapid and simple but lacks adequate sensitivity and specificity; for this reason, DFA is not recommended.

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Isolation of Kerstersia gyiorum from a Patient with Cholesteatomatous Chronic Otitis Media

Cited by 23 publications

References 13 publications

Isolation of culturable aerobic bacteria and evidence of Kerstersia gyiorum from the blowhole of captive Yangtze finless porpoises

Isolation of culturable aerobic bacteria and evidence of Kerstersia gyiorum from the blowhole of captive Yangtze finless porpoises

Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection

Bordetella and Related Genera

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