Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats

Abstract

Reinforcement of the hydroperoxide-eliminating activity in the small and large intestines should prevent associated diseases. We previously isolated a lactic acid bacterium, Pediococcus pentosaceus Be1 that facilitates a 2-electron reduction of hydrogen peroxide to water. In this study, we successfully isolated an alternative lactic acid bacterium, Lactobacillus plantarum P1-2, that can efficiently reduce environmental alkyl hydroperoxides and fatty acid hydroperoxides to their corresponding hydroxyl derivatives through a 2-electron reduction. Each strain exhibited a wide concentration range with regard to the environmental reducing activity for each hydroperoxide. Given this, the two lactic acid bacteria were orally administered to an oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests that P. pentosaceus Be1 and L. plantarum P1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that only L. plantarum P1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress. Citation: Watanabe A, Yamaguchi T, Murota K, Ishii N, Terao J, Okada S, et al. (2020) Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats. PLoS ONE 15(2): e0215113. https://doi.org/10.

Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota

“…This reduction in the TBARS and GSH markers could have protective effects against inflammation. Similar results reported by Watanabe et al, 2020 [57] as concluded the effective of L. plantarum P1-2 and P. pentosaceus Be1 for reducing oxidative stresses in vivo.…”

The Administration Effect of Lactic Acid Bacteria Reducing Environmental Alkyl and Fatty Acid Hydroperoxides on Piglets in the Absence of Antimicrobial Agents and in the Presence of Iron

“…Since LpP1-2 has potent peroxidized fatty acid reducing activity, its administration may have suppressive effect on iron toxicity. Previously, we showed that LpP1-2 must be administered as viable bacteria to exert this activity effectively [9]. The present study demonstrated its effectiveness in pigs.…”

“…Previously, a decrease in lipid peroxidation index was observed in the colonic mucosa of ironoverloaded rats administrated with LpP1-2 [9]. In the present study, TBARS level in serum samples iron-fed piglets in the LABLp-administrated group was lower than that in the nonadministrated group.…”

“…The authors isolated a lactic acid bacterium, ( Lp P1-2), from fermented food. This strain reduces environmental alkyl hydroperoxides and fatty acid hydroperoxides and converts both peroxides to the less toxic hydroxy form via two electron reduction [9]. Oral administration of Lp P1-2 to the oxygen-sensitive short-lived nematode mutant resulted in a significant expansion of its lifespan, suggesting that Lp P1-2 inhibits internal oxidative stress [9].…”

“…This strain reduces environmental alkyl hydroperoxides and fatty acid hydroperoxides and converts both peroxides to the less toxic hydroxy form via two electron reduction [9]. Oral administration of Lp P1-2 to the oxygen-sensitive short-lived nematode mutant resulted in a significant expansion of its lifespan, suggesting that Lp P1-2 inhibits internal oxidative stress [9]. To specify the organs involved in this response, we performed a similar experiment on iron-overloaded rats in which lipid peroxidation was induced.…”