Isolation of Lactic Acid Bacteria from Intestine of Freshwater Fishes and Elucidation of Probiotic Potential for Aquaculture Application

Govindaraj, Krishnaveni; Samayanpaulraj, Vignesh; Vidhyalakshmi, N.; Ramesh, U.

doi:10.1007/s12602-021-09811-6

Cited by 31 publications

(23 citation statements)

References 71 publications

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“…Since then, bacterial lipases have been reported in a diverse range of bacteria isolated from aquacultured species, comprising freshwater, brackish water and marine organisms 30 . In freshwater fish, lipase‐producing bacteria have been revealed in the intestines of many fish species, including grass carp and gibel carp ( Carassius auratus gibelio ), 78,79 yellow catfish ( Pelteobagrus fulvidraco ), 93 M. albus , 91 amur minnow ( Rhynchocypris lagowskii ), 70 some cold‐water fish species, 82 Indian major carp, mrigal carp ( Cirrhinus mrigala ), 85,86 India freshwater teleosts, including striped snakehead ( Channa striatus ), striped dwarf catfish ( Mystus vittatus ), orangefin labeo ( Labeo calbasu ) and mrigal carp 84 and rohu, Labeo rohita 89 . Ariole and co‐authors reported that 61.1% of bacterial isolates isolated from the digestive tract of cultured African catfish ( Clarias gariepinus ) revealed the ability to degrade lipids 59 .…”

Section: How the Intestinal Microbiota Of Fish Regulates Lipid Metabo...mentioning

confidence: 99%

Regulation of lipid metabolism by gut microbiota in aquatic animals

Pan

Zan

et al. 2023

Reviews in Aquaculture

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Lipids are an essential component of living beings and an important group of nutrients. As the gut microbiota plays important roles in the intestinal absorption and extraintestinal metabolism of dietary lipids, the current review addresses the recent progress regarding the interactions between the gut microbiota and lipid metabolism in aquatic animals, with a focus on fish. We discuss in detail how dietary lipid sources and content affect the composition of the gut microbiome and the mechanism by which the gut microbiota affects the lipid metabolism of the host. This interaction is largely mediated via microbial lipases, short‐chain fatty acids and the gut‐liver axis. The latter refers to the metabolism of biliary salts and acids, the regulation of their synthesis by gut microbes and their impact on the lipid metabolism. Finally, we briefly discuss how probiotic supplementation modulates the host's microbiome, and how probiotics have a beneficial effect on health and welfare of farmed aquatic animals. Although the influence of intestinal microbiota on lipid metabolism has been explored before, further research is needed to profoundly investigate the molecular mechanisms by which microbial metabolites (SCFAs and bile acids) induce lipid metabolism.

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Section: How the Intestinal Microbiota Of Fish Regulates Lipid Metabo...mentioning

confidence: 99%

Regulation of lipid metabolism by gut microbiota in aquatic animals

Pan

Zan

et al. 2023

Reviews in Aquaculture

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“…However, LAB exhibited a wide range of antagonistic effects against Gram-positive bacteria. They have minimal effects on Gram-negative bacteria [ 15 ]. Plant-growth-promoting properties were limited in LAB, while their performance was poor compared to other beneficial bacteria and fungi.…”

Section: Limitations and Future Prospects Of Labmentioning

confidence: 99%

“…In addition, LAB culture conditions such as temperature, low pH, and anaerobic conditions inhibit various mold and food-borne pathogens [ 8 ]. Thus, the LAB characterized by antagonistic properties are crucial to countering potential pathogens [ 15 ]. LAB strains are a promising biocontrol agent; they have a plant growth stimulation effect and inhibit phytopathogenic microbes [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Lactic Acid Bacteria (LAB) in Sustainable Agriculture: Advantages and Limitations

Raman

Kim

Choi

et al. 2022

IJMS

103

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Lactic acid bacteria (LAB) are significant groups of probiotic organisms in fermented food and are generally considered safe. LAB regulate soil organic matter and the biochemical cycle, detoxify hazardous chemicals, and enhance plant health. They are found in decomposing plants, traditional fermented milk products, and normal human gastrointestinal and vaginal flora. Exploring LAB identified in unknown niches may lead to isolating unique species. However, their classification is quite complex, and they are adapted to high sugar concentrations and acidic environments. LAB strains are considered promising candidates for sustainable agriculture, and they promote soil health and fertility. Therefore, they have received much attention regarding sustainable agriculture. LAB metabolites promote plant growth and stimulate shoot and root growth. As fertilizers, LAB can promote biodegradation, accelerate the soil organic content, and produce organic acid and bacteriocin metabolites. However, LAB show an antagonistic effect against phytopathogens, inhibiting fungal and bacterial populations in the rhizosphere and phyllosphere. Several studies have proposed the LAB bioremediation efficiency and detoxification of heavy metals and mycotoxins. However, LAB genetic manipulation and metabolic engineered tools provide efficient cell factories tailor-made to produce beneficial industrial and agro-products. This review discusses lactic acid bacteria advantages and limitations in sustainable agricultural development.

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“…The assay of bacterial cell surface hydrophobicity test was carried out according to (Kavitha et al, 2018;Govindaraj et al, 2021) and slightly modified. Briefly, three organic solvents (xylene, chloroform, and ethyl acetate) were used to detect the surface hydrophobicity of selected isolates.…”

Section: Bacterial Cell Surface Hydrophobicitymentioning

confidence: 99%

Screening and Characterization of Potential Antioxidant Probiotics Isolated from the Gut of Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

et al. 2022

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Some symbiotic probiotics have antioxidant activities and could improve the antioxidant capacity of the host. There is still no report on the screening of host-derived antioxidant probiotics for grouper farming. In this study, 369 out of 583 isolates were screened from the gut of hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus♂) based on their non-hemolytic characteristics. Subsequent preliminary screening with 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) scavenging rate assay resulted in 36 potential antioxidant isolates. After comprehensive evaluation with nine different antioxidant assays (DPPH scavenging rate, 2,2’-azino-bis (3-ethylbezothiazoline)-6-sulfonic acid radical scavenging rate, iron ion reducing ability, reducing activity, O2-· scavenging rate,·OH scavenging rate, ferrous ion chelating rate, hydrogen peroxide tolerance, oxygen-free radical scavenging ability, et al.), 10 isolates with strong antioxidant abilities were screened from 36 potential antioxidant isolates. Then some other probiotic properties, such as simulated gastrointestinal fluid tolerance, adhesion, digestive enzyme activity, and antibacterial activity of the 10 selected isolates were evaluated. All 10 isolates were also identified using the molecular method. Finally, Vibrio rhodolitus GO 91 and Shewanella corallii GO 310, as representatives of the two genera resulting from the identification of the 10 isolates, and with the best overall probiotic properties, were selected from the 10 isolates. Isolates GO 91 and GO 310 were further tested for their safety performances. Antibiotic sensitivity tests showed that GO91 and GO310 were sensitive to many commonly used aquaculture antibiotics. The in vivo challenge test of GO 91 and GO 310 didn’t cause any disease symptoms or death in hybrid grouper. Therefore, isolates GO 91 and GO 310 showed great potential to be used as probiotics in hybrid grouper farming.

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Isolation of Lactic Acid Bacteria from Intestine of Freshwater Fishes and Elucidation of Probiotic Potential for Aquaculture Application

Cited by 31 publications

References 71 publications

Regulation of lipid metabolism by gut microbiota in aquatic animals

Regulation of lipid metabolism by gut microbiota in aquatic animals

Application of Lactic Acid Bacteria (LAB) in Sustainable Agriculture: Advantages and Limitations

Screening and Characterization of Potential Antioxidant Probiotics Isolated from the Gut of Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

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