Isolation of lactoferrin and immunoglobulins from dairy whey by an electrodialysis with filtration membrane process

Wang, Q.; Chen, George Q.; Kentish, Sandra E.

doi:10.1016/j.seppur.2019.115987

Cited by 31 publications

(14 citation statements)

References 51 publications

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“…Current research is exploring other ways to optimize hydrolysis before EDUF, e.g., by using high hydrostatic pressure [ 243 ]. Recently, Wang et al [ 244 ] synthesized a polyvinylalcohol (PVA) membrane aiming at a 150-kDa MWCO for use as a filtration membrane in an EDUF stack. They experimented on artificial milk in order to selectively recover glycoproteins (lactoferrin and immunoglobulins) and observed promising concentration in the retentate compartment compared to other dairy proteins tested.…”

Section: Recent Technological Developments Based On Ed Membrane Phmentioning

confidence: 99%

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Bazinet

Geoffroy

2020

Membranes

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In the context of preserving and improving human health, electrodialytic processes are very promising perspectives. Indeed, they allow the treatment of water, preservation of food products, production of bioactive compounds, extraction of organic acids, and recovery of energy from natural and wastewaters without major environmental impact. Hence, the aim of the present review is to give a global portrait of the most recent developments in electrodialytic membrane phenomena and their uses in sustainable strategies. It has appeared that new knowledge on pulsed electric fields, electroconvective vortices, overlimiting conditions and reversal modes as well as recent demonstrations of their applications are currently boosting the interest for electrodialytic processes. However, the hurdles are still high when dealing with scale-ups and real-life conditions. Furthermore, looking at the recent research trends, potable water and wastewater treatment as well as the production of value-added bioactive products in a circular economy will probably be the main applications to be developed and improved. All these processes, taking into account their principles and specificities, can be used for specific eco-efficient applications. However, to prove the sustainability of such process strategies, more life cycle assessments will be necessary to convince people of the merits of coupling these technologies.

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Section: Recent Technological Developments Based On Ed Membrane Phmentioning

confidence: 99%

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Bazinet

Geoffroy

2020

Membranes

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“…However, the selectivity between β-Lg and LF was low, due to the simultaneous migration of proteins across the membrane in the process. 64 Recently, Wang et al 65 developed an electrodialysis with filtration membrane process that is used with polyvinyl alcohol membranes. This method could separate LF and BSA on the basis of the large aggregation sizes of LF (MWs of ∼300 kDa) compared with those of BSA (MWs of ∼66 kDa).…”

Section: Separation and Purification Technologies Of Milk Bioactivesmentioning

confidence: 99%

Bioactives in bovine milk: chemistry, technology, and applications

et al. 2021

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The significance of dairy in human health and nutrition is gaining significant momentum as consumers continue to desire wholesome, nutritious foods to fulfill their health and wellness needs. Bovine milk not only consists of all the essential nutrients required for growth and development, it also provides a broad range of bioactive components that play an important role in managing human homeostasis and immune function. In recent years, milk bioactives, including α-lactalbumin, lactoferrin, glycomacropeptide, milk fat globule membrane, and milk oligosaccharides, have been intensively studied because of their unique bioactivity and functionality. Challenges for the application of these bioactive components in food and pharmaceutical formulations are associated with their isolation and purification on an industrial scale and also with their physical and chemical instability during processing, storage, and digestion. These challenges can be overcome by advanced separation techniques and sophisticated nano- or micro-encapsulation technologies. Current knowledge about the chemistry, separation, and encapsulation technology of major bioactives derived from bovine milk and their application in the food industry is reviewed here.

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“…The negative electric charge bound in/on the cation‐exchange membranes electrostatically repels anions, including both reaction products, and prevents their escape from the permeate stream. Ion‐exchange membranes have been recently used to provide conductive coupling in a membrane device developed for the separation of selected proteins from whey (Wang et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Ionexchange membranes have been recently used to provide conductive F I G U R E 1 (a) Scheme of the membrane micro-contactor; (b) scheme of the combined microreactor-microseparator for continuous flow synthesis of 6-aminopenicillanic acid. The combined system consists of A-the reservoir of the permeate phase, B-the reservoir of the Penicillin G solution, C-the reservoir of the enzyme solution, D-a three-way connector, E-a reaction capillary immersed in the water bath, F-a membrane microcontactor, G-the retentate reservoir, H-the permeate reservoir [Color figure can be viewed at wileyonlinelibrary.com] coupling in a membrane device developed for the separation of selected proteins from whey (Wang et al, 2020).…”

Section: Microextractor Operationmentioning

confidence: 99%

Electric‐field‐enhanced selective separation of products of an enzymatic reaction in a membrane micro‐contactor

Romanov

Slouka

Přibyl

2020

Biotech & Bioengineering

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Processes employed in separations of products of enzyme reactions are often driven by diffusion, and their efficiency can be limited. Here, we exploit the effect of a direct current (DC) electric field that intensifies mass transfer through a semipermeable membrane for fast, continuous, and selective separation of electrically charged molecules. Specifically, we separate low‐molecular‐weight reaction products (phenylacetic acid, 6‐aminopenicillanic acid) from the original reaction mixture containing a free enzyme (penicillin acylase). The developed microfluidic dialysis‐membrane contactor allows a stable counter‐current arrangement of the retentate and permeates liquid streams on which DC electric field is perpendicularly applied. The applied electric field significantly accelerates the transport of electrically charged products through the semipermeable membrane yielding high separation efficiencies at short residence times. The residence time of 5 min is sufficient to reach 100% separation yield in the electric field. The same residence time provides only a 50% yield in the diffusion‐controlled experiments. We experimentally demonstrated that a combined microreactor–microextractor with a recycle of the soluble penicillin acylase can continuously produce both the reaction products at high concentrations. The developed membrane‐contactor is a versatile platform allowing to tune its characteristics, such as selectivity given by the membrane, or the type of the retentate phase, for a specific application.

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Isolation of lactoferrin and immunoglobulins from dairy whey by an electrodialysis with filtration membrane process

Cited by 31 publications

References 51 publications

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Electrodialytic Processes: Market Overview, Membrane Phenomena, Recent Developments and Sustainable Strategies

Bioactives in bovine milk: chemistry, technology, and applications

Electric‐field‐enhanced selective separation of products of an enzymatic reaction in a membrane micro‐contactor

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