Isolation of Latent Herpes Simplex Virus from the Superior Cervical and Vagus Ganglions of Human Beings

Warren, K. G.; Brown, Stuart A.; Wróblewska, Zofia; Gilden, D. H.; Koprowski, Hilary; Subak‐Sharpe, J. H.

doi:10.1056/nejm197805112981907

Cited by 250 publications

(102 citation statements)

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“…This gene expression occurred under the control of the LAT promoter during the latent phase of this virus. Like HSV, known to become latent in the sensory ganglia, [19][20][21][22][23] this chimeric HSV-1 may enter the latent phase in the anterior horn motor neurons with expression of ␤-gal activity after transport from the skeletal muscle. The spread of ␤-gal activity in the spinal cord did not further progress later than 14 days after inoculation.…”

Section: Discussionmentioning

confidence: 99%

Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector

et al. 2000

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To clarify the feasibility of the herpes simplex virus (HSV) vector in expressing the foreign gene in the motor neuron, we inoculated a live attenuated HSV expressing ␤-galactosidase (␤-gal) activity under a latency-associated transcript promoter in the right gastrocnemius muscle of rats. Expression of ␤-gal activity was observed 5 days after inoculation in the bilateral anterior horn cells of the spinal cord that innervates the inoculation muscle. However, the spread of ␤-gal activity was not observed in the inoculation muscle. Without significant pathological changes, the spread of ␤-gal-expressing neurons was observed in the lumbosacral

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Section: Discussionmentioning

confidence: 99%

Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector

et al. 2000

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“…Herpes simplex virus type 1 (HSV-1) may be isolated from herpetic facial lesions , some head sensory ganglia (Bastian et al, 1972;Baringer & Swoveland, 1973;Baringer, 1974;Warren et al, 1978), brain of herpes encephalitis cases (Longson & Bailey, 1977;Koskiniemi & Vaheri, 1982), genital regions in a minority of herpes genitalis cases (Peutherer & Smith, 1981 ;Chang, 1977), and herpetic infections at other anatomical locations (e.g. Buss & Schary3, 1979).…”

Section: Introductionmentioning

confidence: 99%

Variable Restriction Endonuclease Sites of Herpes Simplex Virus Type 1 Isolates from Encephalitic, Facial and Genital Lesions and Ganglia

Chaney¹,

Warren

Subak‐Sharpe³

1983

Journal of General Virology

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SUMMARYThe distribution of restriction endonuclease (RE) sites was compared for 84 herpes simplex virus type 1 (HSV-1) isolates obtained from the ganglia, facial lesions, genital lesions and from brain tissue from herpes encephalitis cases. The isolates came from Canada, the U.K., the U.S.A. and Japan. Out of a total of 224 sites identified, 87 were variable. Three of the 30 most variable sites were at significantly (P < 0-05) different frequencies in groups of isolates from distinct anatomical sites of isolation; one of these, and a further two sites, were at significantly different frequencies in groups from distinct geographical origins. There are at least two inter-related linkage groups. However, most of the site combinations appear to be random. The variability of RE sites in contiguous genome segments, which include both non-coding and coding sequences, show a marked heterogeneity, indicating that some viral gene sequences are more variable than others. The three RE sites at different frequencies in viral groups from distinct anatomical sites of isolation are in two genome segments : map units 27 to 35 and 50 to 57. We infer from the observed associations with anatomical site of viral isolation that part of at least one of these segments may modulate viral virulence in man following infection.

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“…This gene expression occurred under the control of the LAT promoter during the latent phase of infection. Like HSV, known to become latent in the sensory ganglia, [26][27][28][29][30] this chimeric HSV-1 may enter the latent phase in the anterior horn motor neurons with expression of ␤-gal activity after transport from the skeletal muscle and interneuronal spread.…”

Section: Discussionmentioning

confidence: 99%

Effect of immunity on gene delivery into anterior horn motor neurons by live attenuated herpes simplex virus vector

et al. 2001

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Isolation of Latent Herpes Simplex Virus from the Superior Cervical and Vagus Ganglions of Human Beings

Cited by 250 publications

References 6 publications

Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector

Long-term gene expression in the anterior horn motor neurons after intramuscular inoculation of a live herpes simplex virus vector

Variable Restriction Endonuclease Sites of Herpes Simplex Virus Type 1 Isolates from Encephalitic, Facial and Genital Lesions and Ganglia

Effect of immunity on gene delivery into anterior horn motor neurons by live attenuated herpes simplex virus vector

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