Isolation of Lysozyme From Egg White

Vinyl sulfone: a versatile function for simple bioconjugation and immobilization

“…S2 †). These results demonstrate that the protein itself influences the extent of the coupling, and that the labeling of proteins with vinyl a Estimated from the sequence, except for lysozyme whose isoelectric point was taken from Alderton et al 13 Scheme 2 Applications of vinyl sulfone-functionalized tags and sugars in bioconjugation of proteins. sulfone-derivatized reagents is feasible regardless of the isoelectric point, number of potential nucleophiles or glycosylation.…”

“…M.p. 88-90 • C; [a] D -42 • (c 1, methanol); 1 H NMR (CD 3 OD, 300 MHz): d 4.42 (d, 1H, J = 9.7 Hz), 3.86 (dd, 1H, J = 12.0 and 1.2 Hz), 3.80-3.60 (m, 5H), 3.18 (t, 1H, J = 8.7 Hz), 3.10 (ddd, 1H, J = 14.0, 9.6 and 6.0 Hz), 2.96 (ddd, 1H, J = 13.9, 9.6 and 6.2 Hz); 13 C NMR (CD 3 OD, 75 MHz): d 87.4, 82.1, 79.6, 74.4, 71.5, 62.9, 44.8, 33.7; HRMS (m/z) (FAB+) calcd. for C 8 H 15 ClO 5 S + Na: 281.0226; found: 281.0222.…”

“…IR spectra were recorded on a Satellite Mattson FTIR. 1 H and 13 C NMR spectra were recorded at room temperature on a Varian Direct Drive (300, 400 and 500 MHz) spectrometer. Chemical shifts are given in ppm and referenced to internal CDCl 3 .…”

“…Removal of the solvent under reduced pressure yielded a crude that was purified by column chromatography (AcOEt-hexane 2 : 1 → 3 : 1) giving 1 (805 mg, 51%) as a syrup. n max (film)/cm -1 : 1608, 1472, 1382, 1310, and 1121; 1 H-NMR (CDCl 3 , 400 MHz): d 6.76 (dd, 2 H, J 16.6 and 9.8 Hz), 6.41 (d, 2H, J 16.6 Hz), 6.10 (d, 2 H, J 9.8 Hz), 3.90 (t, 4 H, J 5.8 Hz), 3.63 (s, 4 H), 3.25 (t, 4 H, J 5.6 Hz); 13…”

Use of the Bruker AXS SMART BREEZE™ System for Macromolecular X-ray Data Collection

“…Hen egg white lysozyme was first isolated and crystallized in 1944. 13 It was the first enzyme to have its structure determined, 14 although the structure wasn't refined until nearly a decade later. 15 Because details of this protein have been studied and very well defined, including crystallization conditions, lysozyme provided a good target to carry out our evaluation experiments.…”