Isolation of Major Pathogens from Cattle with Subclinical Mastitis and Determination of Antibiotic Susceptibilities

Abstract

Bacterial factors of bovine mastitis have two groups according to origin and transmission routes. The primary contagious agents are S. aureus, Streptococcus sp. and E. coli. These factors are spread widely, especially with handling and milking units. The scope of this study was to determine the isolation and antibiotic susceptibility of S. aureus, Streptococcus sp. and E. coli, which are very important for milk production and frequent agents of subclinical mastitis. In our study, 138 samples of dairy cows were taken aseptically from Aydin province with subclinical mastitis case. In conclusion, 102 (18.5%) S. aureus, 21 (3.8%) Streptococcus sp., and 15 (2.7%) E. coli were isolated from the 552 milk samples examined. After PCR with specific primers in the genotypic identification, all isolates were confirmed about bacterial identification. S. aureus, Streptococcus sp., and E. coli isolates were susceptible to antibiotics of amoxicillin-clavulanic acid, oxytetracycline, cefoperazone and cephalexin-kanamycin.

Investigation of antimicrobial resistance of the bacteria isolated from cows with latent mastitis

“…Mastitas -pieno liaukos uždegimas -viena didžiausių problemų pienininkystės ūkiuose (Ugur et al, 2018). Lietuvoje 2015 m. parduo ta 11,9 t veterinarinių antimikrobinių medžiagų (Sales of Veterinary Antimicrobial Agents in 30 European Countries in 2015, 2017).…”