Isolation of Marine Microbugs from Mangrove Ecosystem and Screening of Industrially Important Enzymes

Abstract

Mangrove ecosystem are known for their diverse and rich microbiota. Here human influence is limited, so novelty in bacterial diversity is more common as mangroves ecosystem comes under conservative area and human interference in restricted here. In this present study, mangrove sample like mangrove associated Soil, Coastal water, Roots were collected from Borivali coastal area of manori creek and Jhow Island, Maharashtra under prior permission from CCF, Mangrove Cell, Maharashtra government. Halophiles are in demand in many Biotech based company and their enzymes are equally in demand due their properties. Here in the present experiment, with the help of enriched media 30 samples were processed from which 8 samples were from Jhow Island and 22 samples from borivali coastal-line. All the samples were screened with 10% salt and 20% salt concentration (NaCl). Sample were also screened for 7 pH and 9 pH growth parameters from Borivali and Jhow Island respectively. From above mention samples, total 62 Isolates were obtained, which were screened for Bio-Industrially important enzymes like Amylase, Protease and Cellulase. These enzymes have great demand when they are active in high salt concentration or alkaline pH. These enzymes may emphasize more Scientific-Entrepreneur in India.

Impact of Substrate pH and Enzyme-Substrate Incubation Time on Protease from a New Halo-tolerant Bor S17B13 Found in the Mangroves of Western India

“…The media contain gelatin at a concentration of 10 g/l, peptone at a concentration of 5 g/l, agar at a concentration of 20 g/l, and sodium chloride at concentrations of 10% and 20%. Subsequently the plates were incubated for another 48 hours at room temperature after that plates were examined for hydrolyzed zone by overlay them with Frazier reagent (Composed of HgCl 2 , 150g/L and concentrated HCl, 200 ml/L) for protease [6] and isolates were also screened for amylase and cellulase ( [7][8][9]).…”

“…From them protease enzyme producer were selected for further studies as protease enzyme stay sable for long time medium and wide application. The final selected isolate was Bor S17B13, which was maximum producer of protease from the isolates on the basis of hydrolysis zone on plate [6].…”

Morphological Dominance of Isolates at Two Sites With Less Humanoid Interference in Maharashtra, India

“…Isolates were observed under light microscope, then screen for protease, amylase , cellulose enzymes, from these potential isolate Bor S17B13 was identified by molecular identification (Sawant & Thumar, 2022), ("Microb. Enzym.…”