Isolation of Mercury-binding Peptides in Vegetative Parts of Chromolaena odorata

Velasco-Alinsug, Malona P.; Rivero, G.C.; Quibuyen, Titos Anacleto O.

doi:10.1515/znc-2005-3-409

Cited by 16 publications

(11 citation statements)

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“…of the Asteraceae family, commonly known as Siam weed, is abundantly present in different agricultural systems (Roder et al 1995;MacFadyen and Cruttwell 1996). Bioaccumulation pattern and chemistry of the mode and type of accumulation of Hg have already been reported in C. odorata (Velasco-Alinsug et al 2005;Swapna et al 2014). According to Agunbiade and Fawale (2009), Siam weed can be used as biomarker in assessing metal contaminations in the traffic-and solid waste-polluted areas.…”

Section: Introductionmentioning

confidence: 78%

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Structural changes in response to bioaccumulation of iron and mercury in Chromolaena odorata (L.) King & Robins.

Salim

Chandra

et al. 2015

Environ Monit Assess

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A comparative study was designed to elucidate the effect of iron and mercury on the morphological and anatomical changes as well as bioaccumulation potential in Chromolaena odorata. Plants were grown in half-strength Hoagland nutrient medium artificially contaminated with known quantities of HgCl2 (15 μM) and FeCl3 (1000 μM). Bioaccumulation of Hg and Fe was maximum in the root, and comparatively reduced bioaccumulation was recorded in the stem and leaves. Microscopic studies on morphology and anatomy revealed development of trichomes and lenticels on the stem and modified trichomes on leaves. Localized deposits of stained masses in various internal parts of the root, stem and leaf also were observed. Differential adaptation/strategy of C. odorata to attain tolerance towards Hg and Fe and phytoremediation potential of the plant is discussed.

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Section: Introductionmentioning

confidence: 78%

“…Owing to the mode of cultivation, i.e. hydroponics which contains all nutrients in optimal conditions inclusive of chelators, absorption and translocation of the elements also get enhanced as opined by several authors (Sinha 1999;Velasco-Alinsug et al 2005;Singh et al 2009). …”

Section: Resultsmentioning

confidence: 99%

Structural changes in response to bioaccumulation of iron and mercury in Chromolaena odorata (L.) King & Robins.

Salim

Chandra

et al. 2015

Environ Monit Assess

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“…In equimolar LO to metal ratio [2:metal=1:1] As previously reported by others, ESI-MS is a sensitive technique for demonstrating interactions between metal ions and peptides (D'Agostino et al, 1996;Rubino et al, 2004;Velasco-Alinsug et al, 2005;Chekmeneva et al, 2010;Wilken et al, 2010) or amino acids (Burford et al, 2004). In addition, ESI-MS has been used to study metal ion binding to amino acids and small model peptides (Armentrout et al, 2012;Coates et al, 2016;Peckelsen et al, 2017).…”

Section: Dilution Experiments Of 2 With Four Metal Saltsmentioning

confidence: 99%

Metal binding novel flaxseed peptides (linusorbs)

Jadhav

Zuo

Shim

et al. 2018

Int J of Food Sci Tech

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Summary Linusorbs (LOs; a.k.a. cyclolinopeptides) are naturally occurring orbitides derived from flaxseed. These compounds consist of 8–10 amino acid residues, which are linked via an N‐ to C‐terminal peptide bond with molecular masses of approximately 1 kDa. The LO circular structure makes them candidates for metal binding studies. Flaxseed extracts are known to suppress Pb and Cd toxicity. Hence, four metal salts surveyed include Zn(CH3COO)2, ZnSO4, Pb(CH3COO)2 and Cd(NO3)2 with pure LOs 1–5. Proton NMR spectra indicated interaction of LOs with metal salts in solution and were used to determine impacts of methionine oxidation on interactions with metal ions. The methyl group of methionine S,S‐dioxide of related LOs did not show the same shift in the presence of Zn(CH3COO)2 and Pb(CH3COO)2 observed in their methionine S‐oxide analogues. Metal complexes were observed forming at 10−2 m to 10−4 m but not at lower concentrations (10−5 m to 10−8 m). Mass spectrometry data confirmed that metal binding strength varied by metal in the order Zn(CH3COO)2

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“…The method of Hernandez et al (1998) as modified by Josue et al (2004) was adopted for the differential centrifugation of the crude subcellular fractions. Previous work showed that the leaves from C. odorata plants had the highest accumulation of Hgbinding biomolecules (Velasco-Alinsug et al, 2005b). A gram of FW tissue samples from the leaves of control and Hg-exposed C. odorata plants were separately homogenized in 20 ml extraction buffer pH 7.5.…”

Section: Differential Centrifugationmentioning

confidence: 99%

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2013

J.DEGRADE.MIN. LAND MANAGE.

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Chromolaena odorata (L.f.) R.M. King et H. Robinson plants were grown in Hoagland's solutions with 0.00 ppm and 1.00 ppm Hg(NO 3) 2. The calcium, magnesium, iron, and sulfur levels in the leaves were found to be not significantly affected by presence of the uptaken Hg 2+. The chlorophyll a, chlorophyll b, and total chlorophyll contents of its leaves also remained within normal levels, which may indicate that the photosynthetic machinery of the Hg-exposed C. odorata was unaffected by the presence of Hg 2+. The results of the ICP-AES analyses of the Hg 2+ contents established the presence of Hg 2+ in all the subcellular components obtained from the leaves of the Hg-treated C. odorata plants, and that the ultimate localization of Hg 2+ is in the vacuoles. The findings revealed no significant differences in the degree of oxidative injury between the cells from the control and Hg-treated plants, as evidenced by the low lipid peroxidation levels obtained with the TBARS assay. The SH-containing biomolecules that were initially detected through DTNB assay manifested a predominant peak in the RP-HPLC chromatographs of both the control and Hg-treated plants, with their retention times falling within the ranges of GSH, MT, and cysteine standards. However, the concentrations of the GSH-and/or MT-like, Cys-containing biomolecules detected in the leaves of Hg-treated C. odorata plants were ten times higher than those of the control. The findings of this study suggest that the enhanced antioxidative capacity, the production of Hg-binding biomolecules, and the localization of Hg 2+ ions ultimately in the vacuoles of the leaves are the mechanisms which bring about Hg 2+ tolerance and homeostasis in C. odorata plant. These results indicate that C. odorata is a potentially effective phytoremediator for Hg 2+ .

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Isolation of Mercury-binding Peptides in Vegetative Parts of Chromolaena odorata

Cited by 16 publications

References 0 publications

Structural changes in response to bioaccumulation of iron and mercury in Chromolaena odorata (L.) King & Robins.

Structural changes in response to bioaccumulation of iron and mercury in Chromolaena odorata (L.) King & Robins.

Metal binding novel flaxseed peptides (linusorbs)

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