Isolation of Mhc Class II DMA and DMB cDNA Sequences in a Marsupial: The Gray Short-Tailed Opossum (Monodelphis domestica)

Ó'hUigín, Colm; Sültmann, Holger; Tichy, Herbert; Murray, Brent W.

doi:10.1007/pl00006414

Cited by 19 publications

(9 citation statements)

References 18 publications

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“…Some cartilaginous fish β2M have potential N-glycosylation sites that are rare in tetrapods but present in several bony fish species (32). Consistent with previous studies (33, 34), phylogenetic tree analysis revealed that cartilaginous fish β2M clustered with the orthologous proteins and to the IgSF domains of MHC class IIA/DMA, suggesting that they share the most recent common ancestor (Fig. 1A).…”

Section: Resultssupporting

confidence: 91%

Primordial Linkage ofβ2-Microglobulinto the MHC

Ohta

Shiina

Lohr

et al. 2011

The Journal of Immunology

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β2-Microglobulin (β2M) is believed to have arisen in a basal jawed vertebrate (gnathostome) and is the essential L chain that associates with most MHC class I molecules. It contains a distinctive molecular structure called a constant-1 Ig superfamily domain, which is shared with other adaptive immune molecules including MHC class I and class II. Despite its structural similarity to class I and class II and its conserved function, β2M is encoded outside the MHC in all examined species from bony fish to mammals, but it is assumed to have translocated from its original location within the MHC early in gnathostome evolution. We screened a nurse shark bacterial artificial chromosome library and isolated clones containing β2M genes. A gene present in the MHC of all other vertebrates (ring3) was found in the bacterial artificial chromosome clone, and the close linkage of ring3 and β2M to MHC class I and class II genes was determined by single-strand conformational polymorphism and allele-specific PCR. This study satisfies the long-held conjecture that β2M was linked to the primordial MHC (Ur MHC); furthermore, the apparent stability of the shark genome may yield other genes predicted to have had a primordial association with the MHC specifically and with immunity in general.

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Section: Resultssupporting

confidence: 91%

Primordial Linkage ofβ2-Microglobulinto the MHC

Ohta

Shiina

Lohr

et al. 2011

The Journal of Immunology

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“…MHC genes characterised so far in marsupials include TNF (Wedlock et al, 1996), DRB, an MHC class II gene (Lam et al, 2001a) and an MHC class I gene (Lam et al, 2001b) in the Australian brushtail possum (Trichosurus vulpecula), LTB, TNF, and LTA Deane, 1999, 2000; and the class II DBB (Browning et al, 2004) in the tammar wallaby (Macropus eugenii), partial sequences for a number of MHC I loci in the koala (Phascolarctos cinereus) (Houlden et al, 1996), two class II beta-chain genes from the red-necked wallaby (Macropus rufogriseus) (Slade and Mayer, 1995) and class I (Miska and Miller, 1999) and class II genes (O'hUigin et al, 1998;Stone et al, 1999) in the grey shorttailed Brazilian opossum (Monodelphis domestica). All these genes have been characterized from cDNA, so their genomic context is not known.…”

confidence: 99%

Analysis of the genomic region containing the tammar wallaby <i>(Macropus eugenii)</i> orthologues of MHC class III genes

Cross

Harrison²,

Coggill³

et al. 2005

Cytogenet Genome Res

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Major histocompatibility complex (MHC) molecules are central to development and regulation of the immune system in all jawed vertebrates. MHC class III cytokine genes from the tumor necrosis factor core family, including tumor necrosis factor (TNF) and lymphotoxin alpha and beta (LTA, LTB), are well studied in human and mouse. Orthologues have been identified in several other eutherian species and the cDNA sequences have been reported for a model marsupial, the tammar wallaby. Comparative genomics can help to determine gene function, to understand the evolution of a gene or gene family, and to identify potential regulatory regions. We therefore cloned the genomic region containing the tammar LTB, TNF, and LTA orthologues by “genome walking”, using primers designed from known tammar sequences and regions conserved in other species. We isolated two tammar BAC clones containing all three genes. These tammar genes show similar intergenic distances and the same transcriptional orientation as in human and mouse. Gene structures and sequences are also very conserved. By comparing the tammar, human and mouse genomic sequences we were able to identify candidate regulatory regions for these genes in mammals. Full length sequencing of BACs containing the three genes has been partially completed, and reveals the presence of a number of other tammar MHC III orthologues in this region.

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“…Locus homology inferences cDNA sequences for M. domestica MHC genes UA1 , DAB , DMA, and DMB were obtained from published sources (Miska and Miller, 1999 for UA1 ; Stone et al, 1999 for DAB ;O'hUigin et al, 1998 for DMA and DMB ). The M. domestica cDNA sequence coding the prolactin ( PRL ) precursor was obtained from GenBank (accession no.…”

Section: Mapping Resourcesmentioning

confidence: 99%

“…Based on sequence and structural analysis, UA1 , UB and UC are extremely similar and are likely the products of recent gene duplication events (Miska et al, 2004). Three MHC Class II genes have also been isolated: DAB (Stone et al, 1999), DMA and DMB (O'hUigin et al, 1998). Phylogenetic analyses suggest that DMA and DMB are orthologous to eutherian genes (O'hUigin et al, 1998), while the Class I genes and DAB are not (Miska and Miller, 1999;Belov et al, 2004;Miska et al, 2004).…”

mentioning

confidence: 99%

“…Three MHC Class II genes have also been isolated: DAB (Stone et al, 1999), DMA and DMB (O'hUigin et al, 1998). Phylogenetic analyses suggest that DMA and DMB are orthologous to eutherian genes (O'hUigin et al, 1998), while the Class I genes and DAB are not (Miska and Miller, 1999;Belov et al, 2004;Miska et al, 2004). Using radiolabeledprobe in situ hybridization methods, Slade and co-workers (1994) were able to localize an expressed MHC Class II gene to the long arm of tammar wallaby ( Macropus eugenii ) chromosome 1; but until now no extensive work on the chromosomal location and physical arrangement of the various classes of MHC genes has been undertaken for any marsupial genome.…”

mentioning

confidence: 99%

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Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial <i>Monodelphis domestica</i>

Gouin

Deakin

Miska

et al. 2006

Cytogenet Genome Res

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We used genetic linkage mapping and fluorescence in situhybridization (FISH) to conduct the first analysis of genic organization and chromosome localization of the major histocompatibility complex (MHC) of a marsupial, the gray, short-tailed opossum Monodelphis domestica. Family based linkage analyses of two M. domestica MHC Class I genes (UA1, UG) and three MHC Class II genes (DAB, DMA, and DMB) revealed that these genes were tightly linked and positioned in the central region of linkage group 3 (LG3). This cluster of MHC genes was physically mapped to the centromeric region of chromosome 2q by FISH using a BAC clone containing the UA1 gene. An interesting finding from the linkage analyses is that sex-specific recombination rates were virtually identical within the MHC region. This stands in stark contrast to the genome-wide situation, wherein males exhibit approximately twice as much recombination as females, and could have evolutionary implications for maintaining equality between males and females in the ability to generate haplotype diversity in this region. These analyses also showed that three non-MHC genes that flank the MHC region on human chromosome 6, myelin oligodendrocyte glycoprotein (MOG), bone morphogenetic protein 6 (BMP6), and prolactin (PRL), are split among two separate linkage groups (chromosomes) in M. domestica. Comparative analysis with eight other vertebrate species suggests strong conservation of the BMP6–PRL synteny among birds and mammals, although the BMP6–PRL–MHC–ME1 synteny is not conserved.

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Isolation of Mhc Class II DMA and DMB cDNA Sequences in a Marsupial: The Gray Short-Tailed Opossum (Monodelphis domestica)

Cited by 19 publications

References 18 publications

Primordial Linkage ofβ2-Microglobulinto the MHC

Primordial Linkage ofβ2-Microglobulinto the MHC

Analysis of the genomic region containing the tammar wallaby <i>(Macropus eugenii)</i> orthologues of MHC class III genes

Linkage mapping and physical localization of the major histocompatibility complex region of the marsupial <i>Monodelphis domestica</i>

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