Isolation of Moderately Halophilic Pseudoalteromonas Producing Extracellular Hydrolytic Enzymes from Persian Gulf

Abstract

Extracellular hydrolytic enzymes such as amylases, proteases, lipases and DNases have quite diverse potential usages in different areas such as food industry, biomedical sciences and chemical industries, also it would be of great importance to have available enzymes showing optimal activities at different values of salt concentrations and temperature. Halophiles are the most likely source of such enzymes, because not only their enzymes are salttolerant, but many are also thermotolerant. The purpose of this study was isolation of hydrolytic extracellular enzyme producing halophilic bacteria from water and sediment of the Persian Gulf. Isolated bacteria from water and sediment were inoculated in media with concentration of 0-20% NaCl to determine the optimum salt concentration for growth, isolates were also inoculated in 4 types of solid medium containing substrates of 3 extracellular hydrolytic enzymes including amylase, Protease and Lipase, to determine the quantitative detection of enzyme production, selected strains after more accurate physiological and biochemical studies were identified regarding phylogeny and molecular characteristics using 16S rRNA technique. Isolated enzyme producing bacteria belong to Pseudoalteromonas genera.

Kinetics of Halophilic Enzymes

“…This interest is due to their capacity to efficiently catalyze a process and show optimal activities at different salt concentrations. Halophiles are the most probable source of extremozymes, since they are also capable of tolerating alkaline pH and high temperatures, as reported by several authors [68][69][70][71][72][73][74][75][76][77][78][79].…”

“…The authors identified the genera Marinobacter, Virgibacillus, Halobacillus, Geomicrobium, Chromohalobacter, Oceanobacillus, Bacillus, Halomonas, and Staphylococcus as having hydrolase activities of industrial relevance, pointing out the presence of amylases, lipases, and proteases. A new genus of marine bacteria is included among halophiles that are capable of producing extracellular hydrolytic enzymes, according to the research by Ardakani et al [69], who isolated extracellular hydrolytic enzymes from the water and sediments of the Persian Gulf, in that site the isolation of bacteria that produced enzymes belong to the Pseudoalteromonas genera, and the activities include amylase, protease, and lipase.…”

Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials

“…Diversity of culturable protease-producing bacteria from many marine environments have been investigated, including seawater and offshore sediments (Ardakani 2012;Zhang et al 2015Zhang et al , 2019, deep sea (Liu et al 2018;Xiong et al 2007;Zhou et al 2009), Antarctic (Cristóbal et al 2011;Olivera et al 2007;Zhou et al 2013), and Arctic (Kim et al 2010;Park et al 2014) regions. Pseudoalteromonas is often an abundant group in these investigations, even the most abundant group in some (Kim et al 2010;Liu et al 2018;Zhang et al 2019).…”