Immunological Methods 1979
DOI: 10.1016/b978-0-12-442750-1.50011-5
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Isolation of Monoclonal Antibody by Preparative Isoelectric Focusing in Horizontal Layers of Sephadex G-75

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Cited by 8 publications
(4 citation statements)
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“…This was performed in horizontal layers of Sephadex G-75 using an LKB system (LKB Instruments, Inc., Rockville, Md.) (16). After dialysis (against distilled water) of active material eluted from DEAE-cellulose, 76 ml of material was added to a solution containing 4 ml of Ampholytes (pH 3.5-8) and 4 gm Ultrodex (both from LKB Instruments, Inc.) before application to a gel tray.…”
Section: Purification Of Antigen-specific Suppressor Polypeptidesmentioning
confidence: 99%
“…This was performed in horizontal layers of Sephadex G-75 using an LKB system (LKB Instruments, Inc., Rockville, Md.) (16). After dialysis (against distilled water) of active material eluted from DEAE-cellulose, 76 ml of material was added to a solution containing 4 ml of Ampholytes (pH 3.5-8) and 4 gm Ultrodex (both from LKB Instruments, Inc.) before application to a gel tray.…”
Section: Purification Of Antigen-specific Suppressor Polypeptidesmentioning
confidence: 99%
“…(c) IEF. Flat bed IEF of Con A supernate was performed in horizontal layers of Sephadex (13). Samples were prepared in a 100-ml solution containing 1% glycine and 2% Ampholines (pH 3-10, LKB Produkter).…”
Section: Biochemical Pure'cation Of Lymphokinesmentioning
confidence: 99%
“…The results, now obtained by equilibrium dialysis on the binding of 125I-Av-CHO to a low affinity single-band antibody (K116-700 pIEF 17, Table II, Fig. 2A) (11), yielded a value of 0.77 × 10 -6 M for the dissociation constant. This value was in good agreement with a KD = 1.78 X 10 -6 M I~y fluorescence titration.…”
Section: Distribution Of Affinities In Av-cho-specific Antisera Agarmentioning
confidence: 91%
“…Antibody was purified from whole immune sera first by preparative agarose block electrophoresis (4), and then by one or more runs of pIEF in horizontal layers (11).…”
mentioning
confidence: 99%