Isolation of multipotent mesenchymal stem cells from umbilical cord blood

Immortalization of Human Fetal Cells: The Life Span of Umbilical Cord Blood-derived Cells Can Be Prolonged without Manipulating p16INK4a/RB Braking Pathway

“…The surface markers of the UCB-derived cells examined in this study are exactly the same as those of previously reported UCB-derived cells (Lee et al, 2004). Most of the surface markers are the same as those detected in their bone marrow counterparts (Takeda et al, 2004), with both UCBand bone marrow-derived cells being positive for CD29, CD44, CD55, and CD59, and negative for CD34 and CD117.…”

“…The cells established from UCB can be extensively and clonally expanded in vitro while retaining their potential to differentiate into osteoblasts that produce mineralized matrices and adipocytes that accumulate lipid vacuoles under in vitro conditions. This differentiation potential of the UCB-derived cells is the same as that reported for bone marrow MSCs (Goodwin et al, 2001;Lee et al, 2004).…”

“…Umbilical cord blood (UCB) contains circulating stem/progenitor cells, and the cells contained in UCB are known to be distinct from those contained in bone marrow and adult peripheral blood (Mayani and Lansdorp, 1998). Isolation, characterization, and differentiation of clonally expanded hMSCs derived from UCB (UCBMSCs) have been reported (Goodwin et al, 2001;Lee et al, 2004), and UCBMSCs have been found to have multipotency, and the immunophenotype of the clonally expanded cells is consistent with that reported for bone marrow mesenchymal stem cells. Even now, most UCB is regarded as medical waste in the delivery rooms.…”

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

“…These pluripotent stem cells have the capability to proliferate and self-renew over long periods of time and to differentiate toward almost all cell types. Mesenchymal stem cells (MSCs) may be isolated from a number of adult tissues, including bone marrow, fat, umbilical cord blood, and muscle [5][6][7][8][9][10], and have the capability to proliferate, differentiate, and form a number of mesenchymal tissues. Although MSCs are entering clinical trials for cardiac and orthopedic applications, these cells still have a limited expansion and differentiation capacity [11,12].…”

Mesenchymal stem cells from cryopreserved human umbilical cord blood

“…On the contrary, fresh UCB harvests proved to give adherent expanded cells exhibiting phenotypes of mesenchymal progenitor cells [15]. In addition, immunophenotypes of clonally expanded cells derived from fresh UCB were similar to those of BMderived MSCs, strongly expressing SH2, SH3, and SH4 [16]. In spite of these reported evidences, the presence of MSCs in UCB is still in controversy.…”