Isolation of Mycoplasma hyopneumoniae from different sampling sites in experimentally infected and contact SPF piglets

Marois, C.; Carrou, Jérôme Le; Kobisch, Marylène; Gautier-Bouchardon, Anne V.

doi:10.1016/j.vetmic.2006.10.015

Cited by 85 publications

(95 citation statements)

References 21 publications

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“…Likewise, the growing Tibetan pigs had the second highest prevalence (72.99%) followed by fattening pigs (57.48%) in our research investigation, which are consistent with these reports. Previous studies have shown that M. hyopneumoniae could be transmitted horizontally between wild boars and domestic pigs, and wild boars have been found to be a potential reservoir of M. hyopneumoniae (Vengust et al, 2006;Marois et al, 2007). Similarly, Tibetan pigs in the present study are as susceptible to M. hyopneumoniae as wild boars and domestic pigs (Sibila et al, 2009).…”

Section: Discussionsupporting

confidence: 69%

First report of Mycoplasma hyopneumoniae seroprevalence in Tibetan pigs in Tibet, China

Zhang¹,

Hui²,

Chun³

et al. 2013

Afr. J. Microbiol. Res.

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Section: Discussionsupporting

confidence: 69%

First report of Mycoplasma hyopneumoniae seroprevalence in Tibetan pigs in Tibet, China

Zhang¹,

Hui²,

Chun³

et al. 2013

Afr. J. Microbiol. Res.

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“…The dissociation constant of the C-terminal fragment F4 P116 for porcine plasminogen is 44 Ϯ 5 nM, which is a physiologically relevant value. The capacity of M. hyopneumoniae to capture host plasminogen at the bacterial surface may facilitate the penetration of this pathogen into host organs via the circulatory system, with M. hyopneumoniae recently being isolated from the liver, kidneys and spleen of swine (35).…”

Section: Discussionmentioning

confidence: 99%

“…The C-terminal lysine in P116, isolation of M. hyopneumoniae in deep tissues (35) and previous studies describing the interaction of the broad spectrum protease plasminogen with Mycoplasma species (36,37) led us to investigate the capacity of M. hyopneumoniae and P116 to bind plasminogen. M. hyopneumoniae was found to bind porcine plasminogen in a saturable and dose-dependent manner (Fig.…”

Section: C-terminal Lysine Of P116 Facilitates Binding To Porcinementioning

confidence: 99%

A Processed Multidomain Mycoplasma hyopneumoniae Adhesin Binds Fibronectin, Plasminogen, and Swine Respiratory Cilia

Seymour¹,

Deutscher

Jenkins

et al. 2010

Journal of Biological Chemistry

134

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Porcine enzootic pneumonia is a chronic respiratory disease that affects swine. The etiological agent of the disease, Mycoplasma hyopneumoniae, is a bacterium that adheres to cilia of the swine respiratory tract, resulting in loss of cilia and epithelial cell damage. A M. hyopneumoniae protein P116, encoded by mhp108, was investigated as a potential adhesin. Examination of P116 expression using proteomic analyses observed P116 as a full-length protein and also as fragments, ranging from 17 to 70 kDa in size. A variety of pathogenic bacterial species have been shown to bind the extracellular matrix component fibronectin as an adherence mechanism. M. hyopneumoniae cells were found to bind fibronectin in a dose-dependent and saturable manner. Surface plasmon resonance was used to show that a recombinant C-terminal domain of P116 bound fibronectin at physiologically relevant concentrations (K D 24 ؎ 6 nM). Plasmin(ogen)-binding proteins are also expressed by many bacterial pathogens, facilitating extracellular matrix degradation. M. hyopneumoniae cells were found to also bind plasminogen in a dose-dependent and saturable manner; the C-terminal domain of P116 binds to plasminogen (K D 44 ؎ 5 nM). Plasminogen binding was abolished when the C-terminal lysine of P116 was deleted, implicating this residue as part of the plasminogen binding site. P116 fragments adhere to the PK15 porcine kidney epithelial-like cell line and swine respiratory cilia. Collectively these data suggest that P116 is an important adhesin and virulence factor of M. hyopneumoniae.

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“…Mhp strain 116 was isolated from an outbreak of enzootic pneumonia in France and cultivated in Friis broth medium (FBM) at 37 C as previously described (Friis, 1975;Marois et al, 2007). The titer of the Mhp cultures was expressed as color changing units per milliliter (CCU/mL).…”

Section: Pathogensmentioning

confidence: 99%

Efficacy of combined vaccination against Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus in dually infected pigs

Bourry

Fablet

Simon

et al. 2015

Veterinary Microbiology

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Isolation of Mycoplasma hyopneumoniae from different sampling sites in experimentally infected and contact SPF piglets

Cited by 85 publications

References 21 publications

First report of Mycoplasma hyopneumoniae seroprevalence in Tibetan pigs in Tibet, China

First report of Mycoplasma hyopneumoniae seroprevalence in Tibetan pigs in Tibet, China

A Processed Multidomain Mycoplasma hyopneumoniae Adhesin Binds Fibronectin, Plasminogen, and Swine Respiratory Cilia

Efficacy of combined vaccination against Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus in dually infected pigs

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