Isolation of New Lymphotropic Retrovirus From Two Siblings With Haemophilia B, One With Aids

Vilmer, E; Rouzioux, Christine; Brun, Florence; Fischer, Alain; Chermann, Jean‐Claude; Barré‐Sinoussi, Françoise; Gazengel, C.; Dauguet, C.; Manigne, P.; Griscelli, C; Montagnier, Luc

doi:10.1016/s0140-6736(84)91275-3

Cited by 221 publications

(48 citation statements)

References 11 publications

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“…The implication of HTLV-IIl as the causative agent was based primarily on the high correlation between development of disease and prior exposure to virus, evidenced by the isolation of virus (1,2) and the detection of antibodies to viral proteins in patient sera (3,4,7). Furthermore, the in vitro effect of HTLV-III on immunologically active normal lymphocytes was found to be consistent with the hematological status of patients observed during the course of disease (1,2,8,9). Here we describe the isolation of infectious virus from 47 AIDS patients, 35 AIDS-related-complex (ARC) patients, and 19 clinically healthy donors at risk for AIDS.…”

mentioning

confidence: 90%

Isolation of infectious human T-cell leukemia/lymphotropic virus type III (HTLV-III) from patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) and from healthy carriers: a study of risk groups and tissue sources.

Salahuddin¹,

Markham²,

Popovič³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

131

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Acquired immunodeficiency syndrome (AIDS) and AIDS-related complex (ARC) are thought to be caused by human T-cell leukemia/lymphotropic virus type III (HTLV-Ill). Since (helper/inducer) lymphocytes were preferentially infected and were subjected to a characteristic cytopathic effect. The availability of multiple isolates of virus from a number of different patients and donors will greatly facilitate the characterization of HTLV-III and the study of possible biological and/or biochemical variants of the virus responsible for the development of AIDS, ARC, and related diseases.

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mentioning

confidence: 90%

Isolation of infectious human T-cell leukemia/lymphotropic virus type III (HTLV-III) from patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) and from healthy carriers: a study of risk groups and tissue sources.

Salahuddin¹,

Markham²,

Popovič³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

131

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“…HTLV type II (HTLV-II) has been isolated from a single patient with T-cell hairy cell leukemia (8). Recently, a third HTLV type (HTLV-III) (9,10), similar or identical to the lymphadenopathy-associated virus (LAV) (11,12), has been isolated from >35% of patients with the acquired immunodeficiency syndrome (AIDS). The finding of antibodies reactive with HTLV-III in 88% of affected individuals has suggested that this virus is requisite for the evolution of this disorder (13).…”

mentioning

confidence: 99%

“…In vitro, HTLV-I and HTLV-II infection transforms these T4 cells (14)(15)(16)(17), whereas infection with HTLV-III (or LAV) produces cytopathic effects and cell death (9,12). In vivo, the malignant cells from patients with ATLL and the patient with T-cell hairy cell leukemia are T4 cells (8,15,18,19), and in patients with fully developed AIDS the number of circulating T4 cells is profoundly reduced (20,21).…”

mentioning

confidence: 99%

Infection of human endothelial cells by human T-cell leukemia virus type I.

Hoxie¹,

Matthews²,

Cines³

1984

Proc. Natl. Acad. Sci. U.S.A.

130

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The effects of the human T-cell leukemia virus type I (HTLV-I) on cultured human endothelial cells were evaluated. Coculture of endothelial monolayers with either irradiated HTLV-producing lymphocytes or cell-free virus resulted in the production of multinucleated syncytia. The development of syncytia was inhibited by sera from patients with adult T-cell leukemia/lymphoma (ATLL). HTLV antigens were present on endothelial syncytia passaged in culture for >3 months as detected by an anti-p19 monoclonal antibody, which detects a core protein of HTLV-I, and by ATLL sera. Moreover, these HTLV-infected endothelial cells were then able to infect and transform normal cord blood T lymphocytes with HTLV. These studies demonstrate that human endothelial cells are susceptible to productive HTLV-I infection in vitro and may have relevance for the spectrum of human disease associated with this family of retroviruses.

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“…The first case of acquired immunodeficiency syndrome (AIDS) in a French haemophiliac was reported by Vilmer et al (1) and allowed the isolation of a virus now considered to be the aetiological agent of the disease. We report the second cast!…”

Section: Second Case Of Aids In a French Haemophiliacmentioning

confidence: 99%