Isolation of New Strains of Bacteria Able to Synthesize 1,3-Propanediol from Glycerol

Abstract

The natural environment is inhabited by many species that exhibit very specific metabolic activities that may find industrial applications. The aim of the study was to select non-pathogenic cultures of bacteria of the genus Clostridium and lactic acid bacteria able to convert glycerol into 1,3-propanediol (1,3-PD). Another aim of this study was to identify the isolates that best produced 1,3-propanediol both from pure and crude glycerol. The most efficient strains identified (Cl. butyricum) were analysed on a bioreactor scale. The aim was to determine temperature conditions on the efficiency and duration of 1,3-PD synthesis. The species Clostridium were identified using amplification of the 16S rRNA coding sequence. A total of 123 isolates (of the genus Clostridium and lactic acid bacteria) were isolated; a vast majority of these were able to synthesize 1,3-PD. The best results were obtained for Cl. butyricum strain DSP1, which was isolated from the rumen of a cow fed with glycerol. The strain efficiency using pure glycerol on bioreactor scale 0.65 mol/mol of glycerol at a temperature of 38˚C and a constant pH of 7.0.

1,3-Propanediol production from crude glycerol by Clostridium butyricum DSP1 in repeated batch

“…There are many reports on various methods of cultivating the Clostridium species for the synthesis of 1,3-PD from crude glycerol [4,12,13,14,15,16,17]. Table 2 shows the values of kinetic parameters obtained by other authors as against the results of the present work.…”

“…It was previously isolated from ruminal fluid and put in the collection of the Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poland, as well as deposited at the Polish Collection of Microorganisms (PCM) [13].…”

Electro‐fermentation triggering population selection in mixed‐culture glycerol fermentation

“…Species from the Enterococcus genus were never reported to produce PDO, even though E. avium along with Enterococcus faecalis , Enterococcus faecium and Enterococcus gallinarum were described as glycerol consumers (Collins et al ., ). This genus is closely related to the Lactobacillaceae family in which some species, such as Lactobacillus reuteri, have already been characterized as PDO producers (Szymanowska‐Powałowska et al ., ; Jolly et al ., ). It is then possible that these two families share the same ability to synthetize PDO from glycerol and further investigations on species belonging to the Enterococcus genus could reveal new species of industrial interest.…”

Hydrogen biorefinery: Potential utilization of the liquid waste from fermentative hydrogen production

“…1, 3-properndiol (PD) is an important platform chemical with global production of more than 200,000 t per annum [28]. Different commercially important applications of PD are as follows: (i) production of polytrimethylene terephthalate, a biodegradable polymer widely used in textile fiber production [29][30][31], (ii) it is also used as monomer for the production of different other polymers, such as polyurethane, and polyether [30,32,33], (iii) it is an important material for the production of different cosmetics, detergents, and adhesives [30,33] and, (iv) for production of dioxanes [34].…”