Isolation of Nonpathogenic Mutants of <i>Fusarium oxysporum</i> f. sp. <i>melonis</i> for Biological Control of Fusarium Wilt in Cucurbits

Freeman, Stanley; Zveibil, A.; Vintal, H.; Maymon, Marcel

doi:10.1094/phyto.2002.92.2.164

Cited by 45 publications

(34 citation statements)

References 30 publications

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“…하지만 멜론의 덩굴쪼김병을 방제할 수 있는 살균제 가 거의 없는 실정이므로 저항성 대목 품종을 이용한 접목 재배 방법이 가장 널리 사용되고 있으나 이것은 노동력이 많 이 소요되는 단점이 있다. 따라서 환경친화적인 저항성 품종 의 재배가 효율적인 방제 방법으로 알려져 있다 (Freeman et al, 2002).…”

Section: Fusarium Oxysporum은 형태상으로는 구별이 되지 않지만unclassified

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Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Lee¹,

Lee²,

Jang³

et al. 2015

HST

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This study was conducted to establish an efficient screening system to identify melon resistant to Fusarium oxysporum f. sp. melonis. F. oyxsporum f. sp. melonis GR was isolated from infected melon plants collected at Goryeong and identified as F. oxysporum f. sp. melonis based on morphological characteristics, molecular analyses, and host-specificity tests on cucurbits including melon, oriental melon, cucumber, and watermelon. In addition, the GR isolate was determined as race 1 based on resistance responses of melon differentials to the fungus. To select optimized medium for mass production of inoculum of F. oxysporum f. sp. melonis GR, six media were tested. The fungus produced the most spores (microconidia) in V8-juice broth. Resistance degrees to the GR isolate of 22 commercial melon cultivars and 6 rootstocks for melon plants were investigated. All tested rootstocks showed no symptoms of Fusarium wilt. Among the tested melon cultivars, only three cultivars were susceptible and the other cultivars displayed moderate to high resistance to the GR isolate. For further study, six melon cultivars (Redqueen, Summercool, Superseji, Asiapapaya, Eolukpapaya, and Asiahwanggeum) showing different degrees of resistance to the fungus were selected. The development of Fusarium wilt on the cultivars was tested according to several conditions such as plant growth stage, root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease. On the basis of the test results, we suggest that an efficient screening method for melon plants resistant to F. oxysporum f. sp. melonis is to remove soil from roots of seven-day-old melon seedlings, to dip the seedlings without cutting in spore suspension of 3 × 10 5 conidia/mL for 30 min, to transplant the inoculated seedlings to plastic pots with horticulture nursery media, and then to cultivate the plants in a growth room at 25 to 28°C for about 3 weeks with 12-hour light per day.

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Section: Fusarium Oxysporum은 형태상으로는 구별이 되지 않지만unclassified

“…덩굴쪼김병을 접종하는 경우 많은 실험에서 1분 미만의 매 우 짧은 시간 동안의 접종으로 병 발생을 유도하고 있으나 (Freeman et al, 2002;Jacobson and Gordon, 1988;Namiki et al, 2000), 멜론 유묘에서 안정적인 F. oxysporum f. sp. …”

Section: 멜론 유묘의 뿌리에 포자 현탁액을 침지하는 방법으로unclassified

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Lee¹,

Lee²,

Jang³

et al. 2015

HST

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“…, race (root-dipping method) (Cohen , 1989;Lee , 2015;Matsumoto , 2011;Namiki , 1998;Wellman, 1939). (Latin Snell, 1986 (Freeman , 2002;Iori , 2001;Zhou Everts, 2007;Zink , 1983), Risser , 1976) Seedlings of melon cultivars were uprooted and the roots were washed gently in water. And then the plants were inoculated with FOM GR by dipping the roots in inoculum suspensions of 1×10 6 conidia/ml and 1×10 7 conidia/ml for 30 minutes and were transplanted into 40-cell plastic trays.…”

Section: Melonis(fom)mentioning

confidence: 99%

Development of an Efficient Simple Mass-Screening Method for Resistant Melon to Fusarium oxysporum f. sp. melonis

Lee¹,

Jang²,

Choi³

et al. 2015

Research in Plant Disease

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This study was conducted to establish a simple mass-screening method for resistant melon to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (FOM). Root-dipping inoculation method has been used to investigate resistance of melon plants to Fusarium wilt. However, the inoculation method requires a lot of labor and time because of complicate procedure. To develop a simple screening method on melon Fusarium wilt, occurrence of Fusarium wilt on susceptible and resistant cultivars of melon according to inoculation method including root-dipping, soil-drenching, tip, and scalpel methods was investigated. Scalpel and tip methods showed more clear resistant and susceptible responses in the melon cultivars than root-dipping inoculation method, but tip method represented slightly variable disease severity. In contrast, in the case of soil-drenching inoculation method, disease severity of the susceptible cultivars was very low. Thus we selected scalpel method as inoculation method of a simple screening method for melon Fusarium wilt. By using the scalpel inoculation method, resistance degrees of the cultivars according to incubation temperature after inoculation (25 and 30 o C) and inoculum concentration (1×10 6 and 1×10 7 conidia/ml) were measured. The resistance or susceptibility of the cultivars was hardly affected by all the tested conditions. To look into the effectiveness of scalpel inoculation methods, resistance of 22 commercial melon cultivars to FOM was compare with root-dipping inoculation method. When the melon cultivars were inoculated by scalpel method, resistance responses of all the tested cultivars were clearly distinguished as by root-dipping method. Taken together, we suggest that an efficient simple mass-screening method for resistant melon plant to Fusarium wilt is to sow the seeds of melon in a pot (70 ml of soil) and to grow the seedlings in a greenhouse (25±5 o C) for 7 days, to cut the root of seedlings with a scalpel and then pour a 10 ml-aliquot of the spore suspension of 1×10 6 conidia/ml on soil. The infected plants were cultivated in a growth room at 25 to 30 o C for about 3 weeks with 12-hr light a day.

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“…cucumerinum (Ahn , 1998;Chen , 2010;Vakalounakis, 1993). , (Freeman , 2002;Sherf MacNab, 1985), (Yang Kim, 1994). (Sherf MacNab, 1985).…”

Section: F Oxysporum 80unclassified

“…3). (Vakalounakis, 1993;1996;Vakalounakis Smardas, 1995) (Freeman Rodriguez, 1993;Freeman , 2002;Zhou , 2010).…”

mentioning

confidence: 99%

Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum

Lee¹,

Kim²,

Jang³

et al. 2014

Research in Plant Disease

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The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or 30 o C than at 20 o C. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of 1.0 × 10 6 -1.0 × 10 7 conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at 25 o C for 3 weeks to develop Fusarium wilt.

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Isolation of Nonpathogenic Mutants of Fusarium oxysporum f. sp. melonis for Biological Control of Fusarium Wilt in Cucurbits

Cited by 45 publications

References 30 publications

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Development of an Efficient Simple Mass-Screening Method for Resistant Melon to Fusarium oxysporum f. sp. melonis

Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum

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