Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology

Dasgupta, Kasturi; Hotton, Sara K.; Belknap, William R.; Syed, Yasra; Dardick, Christopher; Thilmony, Roger; Thomson, James G.

doi:10.1186/s12896-020-00635-w

Cited by 4 publications

(4 citation statements)

References 68 publications

(100 reference statements)

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“…Binary plasmid construction was as previously described ( Dasgupta et al, 2020 ). The GUS gene was replaced by the Ruby ( CsMybA1 ) coding sequence.…”

Section: Methodsmentioning

confidence: 99%

“…To produce colored citrus fruit without need for specific environmental conditions or causing an unfavorable metabolic load and stunting plant growth our lab characterized several candidate promoters from citrus and plum that were found to confer fruit preferential expression in tomato ( Dasgupta et al, 2020 ). The highest levels of transgene expression/activity were detected in fruit tissues including the pericarp, placenta, locule, and columella.…”

Section: Introductionmentioning

confidence: 99%

“…The highest levels of transgene expression/activity were detected in fruit tissues including the pericarp, placenta, locule, and columella. Some of the promoters also exhibited weak activity in various reproductive or vegetative tissues ( Dasgupta et al, 2020 ). The CitWax promoter exhibited expression that was similar to those for the tomato fruit ripening-specific promoters SlE8 and SlPG ( Xu et al, 1996 ; Lau et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

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Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit

et al. 2022

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Tissue specific promoters are important tools for the precise genetic engineering of crop plants. Four fruit-preferential promoters were examined for their ability to confer a novel fruit trait in transgenic Mexican lime (Citrus aurantifolia). The Ruby transcription factor activates fruit anthocyanin accumulation within Moro blood orange and has been shown to function in activating anthocyanin accumulation in heterologous plant species. Although the CitVO1, CitUNK, SlE8, and PamMybA promoters were previously shown to confer strong fruit-preferential expression in transgenic tomato, they exhibited no detectable expression in transgenic Mexican lime trees. In contrast, the CitWax promoter exhibited high fruit-preferential expression of Ruby, conferring strong anthocyanin accumulation within the fruit juice sac tissue and moderate activity in floral/reproductive tissues. In some of the transgenic trees with high levels of flower and fruit anthocyanin accumulation, juvenile leaves also exhibited purple coloration, but the color disappeared as the leaves matured. We show that the CitWax promoter enables the expression of Ruby to produce anthocyanin colored fruit desired by consumers. The production of this antioxidant metabolite increases the fruits nutritional value and may provide added health benefits.

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“…Binary plasmid construction was as previously described ( Dasgupta et al, 2020 ). The GUS gene was replaced by the Ruby ( CsMybA1 ) coding sequence.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit

et al. 2022

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“…A third requirement is that the native target gene must be linked to a suitable regulatory sequence. For that purpose, several citrus-derived promoters are being characterized ( Erpen et al, 2018 ; Dasgupta et al, 2020 ; Bezerra et al, 2021 ).…”

Section: Emerging Technologies: Cis and Intragenesis Trans -Grafting And Gene Editing In Citrusmentioning

confidence: 99%

Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies

et al. 2021

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Citrus are among the most prevailing fruit crops produced worldwide. The implementation of effective and reliable breeding programs is essential for coping with the increasing demands of satisfactory yield and quality of the fruit as well as to deal with the negative impact of fast-spreading diseases. Conventional methods are time-consuming and of difficult application because of inherent factors of citrus biology, such as their prolonged juvenile period and a complex reproductive stage, sometimes presenting infertility, self-incompatibility, parthenocarpy, or polyembryony. Moreover, certain desirable traits are absent from cultivated or wild citrus genotypes. All these features are challenging for the incorporation of the desirable traits. In this regard, genetic engineering technologies offer a series of alternative approaches that allow overcoming the difficulties of conventional breeding programs. This review gives a detailed overview of the currently used strategies for the development of genetically modified citrus. We describe different aspects regarding genotype varieties used, including elite cultivars or extensively used scions and rootstocks. Furthermore, we discuss technical aspects of citrus genetic transformation procedures via Agrobacterium, regular physical methods, and magnetofection. Finally, we describe the selection of explants considering young and mature tissues, protoplast isolation, etc. We also address current protocols and novel approaches for improving the in vitro regeneration process, which is an important bottleneck for citrus genetic transformation. This review also explores alternative emerging transformation strategies applied to citrus species such as transient and tissue localized transformation. New breeding technologies, including cisgenesis, intragenesis, and genome editing by clustered regularly interspaced short palindromic repeats (CRISPR), are also discussed. Other relevant aspects comprising new promoters and reporter genes, marker-free systems, and strategies for induction of early flowering, are also addressed. We provided a future perspective on the use of current and new technologies in citrus and its potential impact on regulatory processes.

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Identification of essential element determining fruit-specific transcriptional activity in the tomato HISTIDINE DECARBOXYLASE A gene promoter

Kim

Park

et al. 2022

Plant Cell Rep

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Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology

Cited by 4 publications

References 68 publications

Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit

Tissue-specific expression of Ruby in Mexican lime (C. aurantifolia) confers anthocyanin accumulation in fruit

Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies

Identification of essential element determining fruit-specific transcriptional activity in the tomato HISTIDINE DECARBOXYLASE A gene promoter

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