Isolation of nucleated cells fraction from umbilical cord blood – choice of method

Abstract

The comparison of different techniques for cord blood processing to ensure the highest cell yield based on the “cost-effectiveness” ratio is important.A study of the effectiveness of nucleated cells fraction isolation from umbilical cord blood samples (n = 2898) using double centrifugation, automatic (Sepax S100, Biosafe) or semi-automatic (MacoPress Smart, MacoPharma) system was performed. These include the determining of nucleated cells number (per ml and total), the number of CD34+ cells, and cell viability before samples treatment, after treatment and before freezing.It was shown that semi-automatic and automatic processing methods were more efficient then the double centrifugation by key quality indicators of cell concentrates: the total number of nucleated cells and nucleated cells output. The semi-automatic method of nucleated cells fraction isolation requires the least time for processing a single sample.Considering these data such a system has been proposed as optimal for daily receipt and processing of cord blood samples in a cord blood bank.