Isolation of oleaginous yeast (<i>Rhodosporidium toruloides</i>) mutants tolerant of sugarcane bagasse hydrolysate

Kitahara, Yuki; Yin, Tie; Zhao, Xuebing; Wachi, Masaaki; Liu, Dehua

doi:10.1080/09168451.2014.882746

Cited by 25 publications

(14 citation statements)

References 23 publications

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“…Wild isolates of R. toruloides can produce lipids and carotenoids from a wide variety of carbon sources including glucose ( Wiebe et al, 2012 ), xylose ( Wiebe et al, 2012 ), and acetate ( Huang et al, 2016 ), as well as complex biomass hydrolysates ( Fei et al, 2016 ). They are relatively tolerant to many forms of stress including osmotic stress ( Singh et al, 2016 ) and growth-inhibiting compounds in biomass hydrolysates ( Hu et al, 2009 ; Kitahara et al, 2014 ). Rhodosporidium toruloides has been engineered to produce lipid-derived bioproducts such as fatty alcohols ( Fillet et al, 2015 ) and erucic acid ( Fillet et al, 2017 ) from synthetic pathways.…”

Section: Introductionmentioning

confidence: 99%

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides

Coradetti

Pinel

Geiselman

et al. 2018

eLife

100

115

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The basidiomycete yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides) accumulates high concentrations of lipids and carotenoids from diverse carbon sources. It has great potential as a model for the cellular biology of lipid droplets and for sustainable chemical production. We developed a method for high-throughput genetics (RB-TDNAseq), using sequence-barcoded Agrobacterium tumefaciens T-DNA insertions. We identified 1,337 putative essential genes with low T-DNA insertion rates. We functionally profiled genes required for fatty acid catabolism and lipid accumulation, validating results with 35 targeted deletion strains. We identified a high-confidence set of 150 genes affecting lipid accumulation, including genes with predicted function in signaling cascades, gene expression, protein modification and vesicular trafficking, autophagy, amino acid synthesis and tRNA modification, and genes of unknown function. These results greatly advance our understanding of lipid metabolism in this oleaginous species and demonstrate a general approach for barcoded mutagenesis that should enable functional genomics in diverse fungi.

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Section: Introductionmentioning

confidence: 99%

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides

Coradetti

Pinel

Geiselman

et al. 2018

eLife

100

115

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“…the mutagenesis with EMS increased the total lipid content of mutant strain from 16.2 ± 2.6% in day 10 to 50.8 ± 6.8% in day 18 representing 1.5 to 2 times higher than that of wild strain (7.6 ± 1.8% in day 10 to 34.0 ± 3.8% in day 18). Kitahara et al [38] reported that all mutant strains of Rhodosporidium toruloides showed higher lipid productivity than the wild type (WT). In this study, the biomass and lipid yield of wild RmTun15 were higher than that for R. mucilaginosa [39].…”

Section: Resultsmentioning

confidence: 99%

Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel

Bessadok

Santulli²,

Brück

et al. 2019

Biotechnol Biofuels

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Background: Among the third-generation biodiesel feed stock, oleaginous marine yeasts are the least studied microorganisms for such purpose.Results: Wild strains yeasts were isolated from various Tunisian marine sources including fish waste (Candida tenuis CtTun15, Debaryomyces hansenii DhTun2015, Trichosporon asahii TaTun15 and Yarrowia lipolytica YlTun15) and seawater (Rhodotorula mucilaginosa RmTun15). Following incubation with ethyl methanesulfonate (EMS: 75 mM) for various periods of time (T15, T30, T45, T60 min), the cell viability of these strains responded differentially according to yeast species. For instance, mutated CtTun15 did not survive after 30 min of EMS treatment; higher resistances were observed in DhTun2015 (45 min), in YlTun15, RmTun15 and in TaTun15 (60 min) but with significant decreased cell viabilities (survival rate: 6.02, 3. 16, 11.22, 11.58, 7.70%, respectively). For all surviving mutated strains, the optima of biomass and lipid yields were detected after 96 h in YPD culture; but derived from strains submitted to different period of EMS incubation. In most mutated strains, the maximum biomass (BP) and lipid (LP) productivities coincided and were observed after 30 min of EMS incubation. Only CtTun15 showed different optima of BP and LP (after 30 min and 15 min, respectively). The fatty acids (FA) compositions considered essential in the prediction of biodiesel criteria; were highly affected by EMS mutagenesis. Essentially, 30-and 45-min EMS incubation induced the highest levels of PUFA and MUFA in YlTun15, RmTun15 and TaTun15 with non-significant differences in the different times. However, CtTun15 and DhTun2015 mutant strains responded differently, with the highest levels of MUFA observed following 15 and 45 min; and that of PUFA after 30 and 45 min, respectively. Conclusion:The methyl-esterification of FA from the three mutated yeast strains (30 min-YlTun15, RmTun15 and TaTun15) yielded biodiesel with physical proprieties consistent with the International Standard System. However, investigations are needed for up-scaling biodiesel production.

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“…The plates were incubated at 30 °C for 3-6 days with regular observation for yeast growth. The obtained single yeast colonies were picked-up and sub-cultured on fresh YEPD medium (20 g/L glucose, 20 g/L peptone, 10 g/L YE, 15 g/L agar and seawater (50% v/v)) and incubated at 30 °C for 48 h to obtain pure cultures [23].…”

Section: Yeast Strains Isolationmentioning

confidence: 99%

Rhodosporidium Sp. DR37: A Novel Strain For Production of Squalene in Optimized Cultivation Conditions

Shakeri

Khoshbasirat

Maleki³

2021

Preprint

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Background: Rhodosporidium strain, a well-known oleaginous yeast, has been widely used as a platform for lipid and carotenoid production. However, the production of squalene for application in lipid-based biofuels is not reported in this strain. Here, we isolated and identified newly strain of Rhodosporidium sp. DR37 and investigated its potential for production of squalene under various cultivation conditions.Results: In the present study, Rhodosporidium sp. DR37 was isolated from mangrove ecosystem and its potential for squalene production was assessed. When Rhodosporidium sp. DR37 was cultivated on non-optimized medium (20 g/L glucose, 5 g/L peptone, 5 g/L YE, 15 g/L agar, seawater (50% v/v), pH 7, 30 °C), 64 mg/L of squalene was produced. Significantly, use of optimized medium (20 g/L sucrose, 5 g/L peptone, seawater (20 % v/v), pH 7, 25 °C) allowed highest squalene accumulation in Rhodosporidium sp. DR37 (619 mg/L). The maximum squalene content was obtained as 21.6% of total lipid in comparison to the non-optimized medium (13.9% of total lipid).Conclusions: This study is the first report to employ marine oleaginous Rhodosporidium sp. DR37 for accumulation of squalene in optimized medium. Our findings provide the potential of Rhodosporidium sp. DR37 for production of squalene as well as lipid and carotenoids for biofuel applications in large scale.

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Isolation of oleaginous yeast (Rhodosporidium toruloides) mutants tolerant of sugarcane bagasse hydrolysate

Cited by 25 publications

References 23 publications

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides

Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel

Rhodosporidium Sp. DR37: A Novel Strain For Production of Squalene in Optimized Cultivation Conditions

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