Isolation of pepper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene

Shin, Ryoung; Lee, Gil Je; Park, Chang Jin; Kim, Tae Yeon; You, Jin Sam; Nam, Young Woo; Paek, Kyung Hee

doi:10.1016/s0168-9452(01)00465-4

Cited by 56 publications

(52 citation statements)

References 49 publications

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“…In the previous study, we isolated N6-11 gene that was one of the genes induced specifically during the incompatible interaction between hot pepper plants and TMV (Shin et al, 2001). The clone, renamed as CaTin1, contains the 775-bp insert encoding a protein of 180 amino acid residues (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…However, CaTin1 transcripts started to accumulate from 24 h after the inoculation with TMV-P 0 and increased until 72 h after the inoculation. As a positive control, the expression pattern of the CaPR1 gene, one of the PR genes isolated from hot pepper, was also monitored (Shin et al, 2001). The induction of CaPR1 gene was observed from 48 h after the inoculation.…”

Section: The Catin1 Expression During the Incompatible Interaction Wimentioning

confidence: 99%

“…The TMV-induced hot pepper (Caspsicum annuum) gene, CaTin1 gene, was isolated as an incompatible interaction-specific gene through the differential screening between TMV inoculated and mock inoculated hot pepper plants (Shin et al, 2001). CaTin1 was characterized further because CaTin1 was induced by ethylene but not by SA or MeJA.…”

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Capsicum annuumTobacco Mosaic Virus-Induced Clone 1 Expression Perturbation Alters the Plant's Response to Ethylene and Interferes with the Redox Homeostasis

Shin

Park

et al. 2004

Plant Physiology

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Capsicum annuum tobacco mosaic virus (TMV)-induced clone 1 (CaTin1) gene was expressed early during incompatible interaction of hot pepper (Caspsicum annuum) plants with TMV and Xanthomonas campestris. RNA-blot analysis showed that CaTin1 gene was expressed only in roots in untreated plants and induced mainly in leaf in response to ethylene, NaCl, and methyl viologen but not by salicylic acid and methyl jasmonate. The ethylene dependence of CaTin1 induction upon TMV inoculation was demonstrated by the decrease of CaTin1 expression in response to several inhibitors of ethylene biosynthesis or its action. Transgenic tobacco (Nicotiana tabacum) plants expressing CaTin1 gene in sense-or antisense-orientation showed interesting characteristics such as the accelerated growth and the enhanced resistance to biotic as well as abiotic stresses. Such characteristics appear to be caused by the elevated level of ethylene and H 2 O 2 . Moreover, in transgenic plants expressing antisense CaTin1 gene, the expression of some pathogenesis-related genes was enhanced constitutively, which may be mainly due to the increased ethylene level. The promoter of CaTin1 has four GCC-boxes, two AT-rich regions, and an elicitor-inducible W-box. The induction of the promoter activity by ethylene depends on GCC-boxes and by TMV on W-box. Taken together, we propose that the CaTin1 up-regulation or down-regulation interferes with the redox balance of plants leading to the altered response to ethylene and biotic as well as abiotic stresses.Multiple preformed antimicrobial compounds contribute to the constitutive defense machinery of plants against pathogenic organisms. In addition, plants can trigger the inducible defense programs upon the perception of invaders. The early signaling molecules leading to R-gene-mediated resistance are ion fluxes, GTP-binding proteins, protein kinases, phosphatases, and phospholipases (Hammond-Kosack and Jones, 1996). Plants also respond to external stimuli such as microbial elicitors of cell death and/or defense responses by changing the calcium influx of the cell (Ebel and Scheel, 1997;Higgins et al., 1998). The increase of cytosolic calcium leads to cell death, which seems necessary for hypersensitive cell death triggered by rust fungi (Xu and Heath, 1998). The calcium channel blocker La 31 , on the other hand, prevents the bacteriainduced hypersensitive response (HR) in soybean (Glycine max) leaves (Levine et al., 1996). The burst in the oxidative metabolism generated within minutes of infection leads to the accumulation of the reactive oxygen intermediates (ROI) such as H 2 O 2 and superoxide anion (O 2 d 2 ), involving NADPH-dependent oxidase. ROI serve as signaling molecules, for example in the recognition of the attack by fungal pathogens (Lamb and Dixon, 1997).Ethylene is widely known to modulate the organ senescence induced by various stress factors such as plant pathogens (Moore et al., 2000), O 3 2 (Pell et al., 1997), and hypoxia (He et al., 1996). O 3 2 induces genes involved in the ethylene biosynthes...

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Section: Resultsmentioning

confidence: 99%

Section: The Catin1 Expression During the Incompatible Interaction Wimentioning

confidence: 99%

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Capsicum annuumTobacco Mosaic Virus-Induced Clone 1 Expression Perturbation Alters the Plant's Response to Ethylene and Interferes with the Redox Homeostasis

Shin

Park

et al. 2004

Plant Physiology

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“…These defense-related proteins include pathogenesisrelated protein 10 (PR-10, N2; Shin et al, 2001), an ABA-responsive protein (N3), precursor b-1,3-glucanase (N4; Gheysen et al, 1990), glucan endoglucosidase (N6), peroxidase (N7; Do et al, 2003), endochitinase (N9), osmotin-like protein precursor (N10), and tobacco mosaic virus-induced protein 1-2 (N11) (see Supplemental Figures 3 and 4 online). In particular, PR protein 10 (N2) and the C. annuum ABA-responsive protein (N3) were strongly induced in pepper leaves during the incompatible interaction with Xcv.…”

Section: Pepper and Bacterial Proteins Differentially Expressed Follomentioning

confidence: 99%

Proteomics and Functional Analyses of Pepper Abscisic Acid–Responsive 1 (ABR1), Which Is Involved in Cell Death and Defense Signaling

2011

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Abscisic acid (ABA) is a key regulator of plant growth and development, as well as plant defense responses. A highthroughput in planta proteome screen identified the pepper (Capsicum annuum) GRAM (for glucosyltransferases, Rab-like GTPase activators, and myotubularins) domain-containing ABA-RESPONSIVE1 (ABR1), which is highly induced by infection with avirulent Xanthomonas campestris pv vesicatoria and also by treatment with ABA. The GRAM domain is essential for the cell death response and for the nuclear localization of ABR1. ABR1 is required for priming cell death and reactive oxygen species production, as well as ABA-salicylic acid (SA) antagonism. Silencing of ABR1 significantly compromised the hypersensitive response but enhanced bacterial pathogen growth and ABA levels in pepper. High levels of ABA in ABR1-silenced plants antagonized the SA levels induced by pathogen infection. Heterologous transgenic expression of ABR1 in Arabidopsis thaliana conferred enhanced resistance to Pseudomonas syringae pv tomato and Hyaloperonospora arabidopsidis infection. The susceptibility of the Arabidopsis ABR1 putative ortholog mutant, abr1, to these pathogens also supports the involvement of ABR1 in disease resistance. Together, these results reveal ABR1 as a novel negative regulator of ABA signaling and suggest that the nuclear ABR1 pool is essential for the cell death induction associated with ABA-SA antagonism.

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“…There is quite some evidence that pathogen infection and jasmonic acid influence the levels of proteinase inhibitors in plants (AGRAWAL et al 2002;GRUDEN et al 1997;KREFT et al 1997;SHIN et al 2001). There is some (AVROVA et al 1999;POMPE-NOVAK et al 2002), but significantly less information available dealing with cysteine proteinases.…”

Section: Introductionmentioning

confidence: 99%

The influence of jasmonic acid on the amount and the distribution of cysteine proteinase PLCP-2 in healthy and PVY<sup>NTN</sup> infected potato plants (Solanum tuberosum L.)

Pompe-Novak¹,

Tušek‐Žnidarič²,

Štrukelj³

et al. 2002

Plant Prot. Sci.

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The localization of cysteine proteinase PLCP-2 was investigated in potato plants (Solanum tuberosum L.) cultivar Désirée by electron microscopy. Healthy and PVY NTN infected potato plants were grown in vitro on media with or without a supplement of jasmonic acid. We had already shown that PLCP-2 is present in leaves, stems, tips of shoots and tips of roots of healthy and PVY NTN infected plants. It was detected in various cell types in protein bodies in vacuoles, in cytoplasm and in cell walls. There were significantly larger amounts of PLCP-2 in plants grown on medium with a supplement of jasmonic acid in both healthy and virus infected plants. More protein bodies in vacuoles were found in plants grown on medium with addition of jasmonic acid.

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Isolation of pepper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene

Cited by 56 publications

References 49 publications

Capsicum annuumTobacco Mosaic Virus-Induced Clone 1 Expression Perturbation Alters the Plant's Response to Ethylene and Interferes with the Redox Homeostasis

Capsicum annuumTobacco Mosaic Virus-Induced Clone 1 Expression Perturbation Alters the Plant's Response to Ethylene and Interferes with the Redox Homeostasis

Proteomics and Functional Analyses of Pepper Abscisic Acid–Responsive 1 (ABR1), Which Is Involved in Cell Death and Defense Signaling

The influence of jasmonic acid on the amount and the distribution of cysteine proteinase PLCP-2 in healthy and PVY<sup>NTN</sup> infected potato plants (Solanum tuberosum L.)

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