Isolation of pesticide-degrading actinomycetes from a contaminated site: Bacterial growth, removal and dechlorination of organochlorine pesticides

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

“…Since acidification in CFEs of each consortium was detected, we assumed that MTX is dominantly degraded by dechlorination and dehydrogenation process. In fact, previous studies support that actinobacteria were able to release chloride ions from liquid culture media contaminated with OP (Benimeli et al 2006;Fuentes et al 2010).…”

“…A3, A6, A12, A14, and M7 were previously isolated from sediments and soil samples contaminated with several organochlorine pesticides (OPs) (Benimeli et al 2003;Fuentes et al 2010). Taxonomic identification of these strains has been confirmed by amplification and partial sequencing of their 16S rDNA genes [GenBank database, accession numbers: AY45953 (M7) (Benimeli et al 2007), GU085104 (A3), GQ867051 (A6), GQ867056 (A12), and GU085105 (A14) (Fuentes et al 2010)]. Streptomyces coelicolor A3 (2) (S. coelicolor) obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ) was also used.…”

“…The metabolic fate of MTX has been well investigated in eukaryotes, especially mammals , although its biodegradation has also been described in fungi and bacteria able to use this kind of pesticide under aerobic or anaerobic conditions (Fogel et al 1982;Lee et al 2006;Yim et al 2008;Keum et al 2009). However, little information is available on the MTX biotransformation by Gram-positive microorganisms such as actinobacteria (De Schrijver and De Mot 1999;Fuentes et al 2010;Lal et al 2010). These microorganisms have a great potential for the biodegradation of organic and inorganic toxic compounds, and several studies have demonstrated oxidation and partial dechlorination and dealkylation of aldrin, DDT and herbicides like metolachlor and atrazine, particularly in Streptomyces (Liu et al 1990;Radosevich et al 1995), the main genus of actinobacteria present in soils (Xu et al 1996).…”

“…These microorganisms have a great potential for the biodegradation of organic and inorganic toxic compounds, and several studies have demonstrated oxidation and partial dechlorination and dealkylation of aldrin, DDT and herbicides like metolachlor and atrazine, particularly in Streptomyces (Liu et al 1990;Radosevich et al 1995), the main genus of actinobacteria present in soils (Xu et al 1996). In previous studies, Benimeli et al (2003Benimeli et al ( , 2006Benimeli et al ( , 2007 and Fuentes et al (2010Fuentes et al ( , 2011 isolated and selected wild-type Streptomyces spp. strains which were able to tolerate and remove lindane (c-hexachlorocyclohexane), another OP, from culture media and soil, as pure and mixed cultures.…”

Removal of Imidacloprid Pesticide by Electrocoagulation Process using Iron and aluminum Electrodes

“…It has successfully been employed for the treatment of different wastewaters such as from hospitals [12], baker's yeast [13], laundries [14], biodiesel [15,16], and slaughter houses [17], wastewaters including surfactants [18], fluoride [19] and heavy metal-containing solutions [20,21]. Different technological processes such as adsorption [22], biodegradation [23,24], nanofiltration [25], electrocoagulation, electrochemical reduction and oxidation, indirect electro-oxidation with strong oxidants, and photocatalytic degradation [26,27], for the removal of pesticides have been reported recently. EC is an electrochemical has attracted increasing interest as a promising powerful method for efficiently removing pesticides from water such as Malathion [28], methyl parathion, atrazine and triazophos [29], Malathion, imidacloprid and chlorpyrifos [30], and abamectin [31].…”