Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil

Park, Jin Hee; Bolan, Nanthi; Megharaj, Mallavarapu; Naidu, Ravi

doi:10.1016/j.jhazmat.2010.09.095

Cited by 225 publications

(122 citation statements)

References 60 publications

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“…The soluble phosphate in the culture supernatant was quantified as described by Park et al (2011). Production of indole-3-acetic acid (IAA) by metal mobilizing isolate was assayed as described by Bric et al (1991) using LB medium with different concentrations of L-tryptophan (0, 1, 2, 3, 4 and 5 mg mL À1 ).…”

Section: Characterization Of Plant Growth Promoting Featuresmentioning

confidence: 99%

Phytoextraction of heavy metal polluted soils using Sedum plumbizincicola inoculated with metal mobilizing Phyllobacterium myrsinacearum RC6b

et al. 2013

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h i g h l i g h t sIsolated Phyllobacterium myrsinacearum RC6b effectively mobilized metals in soils. RC6b inoculation enhanced growth and Cd and Zn uptake of Sedum plumbizincicola. Possible mechanisms are plant beneficial activities and soil metal mobilization. RC6b is a good candidate for microbially assisted phytoremediation. a r t i c l e i n f o t r a c tThe aim of this study was to investigate the effects of metal mobilizing plant-growth beneficial bacterium Phyllobacterium myrsinacearum RC6b on plant growth and Cd, Zn and Pb uptake by Sedum plumbizincicola under laboratory conditions. Among a collection of metal-resistant bacteria, P. myrsinacearum RC6b was specifically chosen as a most favorable metal mobilizer based on its capability of mobilizing high concentrations of Cd, Zn and Pb in soils. P. myrsinacearum RC6b exhibited a high degree of resistance to Cd (350 mg L À1 ), Zn (1000 mg L À1 ) and Pb (1200 mg L À1 ). Furthermore, P. myrsinacearum RC6b showed multiple plant growth beneficial features including the production of 1-aminocyclopropane-1-carboxylic acid deaminase, indole-3-acetic acid, siderophore and solubilization of insoluble phosphate. Inoculation of P. myrsinacearum RC6b significantly increased S. plumbizincicola growth and organ metal concentrations except Pb, which concentration was lower in root and stem of inoculated plants. The results suggest that the metal mobilizing P. myrsinacearum RC6b could be used as an effective inoculant for the improvement of phytoremediation in multi-metal polluted soils.

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Section: Characterization Of Plant Growth Promoting Featuresmentioning

confidence: 99%

Phytoextraction of heavy metal polluted soils using Sedum plumbizincicola inoculated with metal mobilizing Phyllobacterium myrsinacearum RC6b

et al. 2013

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“…Phosphorus biosolubilisation efficiency of this strain was tested in culture medium. The ability of microorganisms (bacteria and fungi) to solubilise P from solid materials occurs via acidification, complexation, and enzymatic reactions (Park et al 2011). One of the potential mechanisms for phosphate solubilisation might be acidification of the medium during the growth of fungi due to the following four processes (Burgstaller and Schinner 1993): (1) the excretion of protons via the proton translocating plasma membrane ATPase; (2) the absorption of nutrients in exchange for protons; (3) the excretion of organic acids; and (4) the production of carbon dioxide by the respiratory activity of the fungus.…”

Section: Discussionmentioning

confidence: 99%

“…Generally, solubilisation of P decreased when the concentration of RP increased. The P solubilisation from RP by microorganisms is apparently associated with acidification and enzymatic reactions (Park et al 2011), so regression analysis was carried out to determine the significance of the relationship between pH, organic acid concentration, and phosphatase activity with P solubilisation at 1% RP concentration on day 9 of incubation time. The regression analysis between solubilised P and pH ( R 2 = 0.61, Figure 5(a)), organic acid ( R 2 = 0.59, Figure 5(b)) and acid phosphatase activity ( R 2 = 0.51, Figure 5(c)) showed a signiﬁcant linear relationship ( p < 0.001).…”

Section: Phosphatase Activitymentioning

confidence: 99%

Isolation and identification of a phosphate solubilising fungus from soil of a phosphate mine in Chaluse, Iran

et al. 2016

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Microbial solubilisation of phosphorus from insoluble phosphates is an environmental friendly and cost effective approach in sustainable soil management. Introducing the indigenous microorganisms to soil requires shorter adaptation period and causes fewer ecological distortions than exogenous microorganisms. This study was conducted to isolate and identify the indigenous fungi for phosphate solubilisation in Mazandaran, Iran. A potent phosphate solubilising fungus was isolated from an Iranian phosphate mine and selected for solubilisation of rock phosphate (RP). The identified fungus was characterised by calmodulin-based polymerase chain reaction method as Aspergillus tubingensis SANRU (Sari Agricultural Sciences and Natural Resources University). The phosphate solubilisation ability of the fungal strain was carried out in shake-flask leaching experiments containing various concentrations of RP (1%, 2%, 4%, or 8% w/v). The maximum P solubilisation rate of 347 mg/l was achieved at 1% of RP concentration on day 9. The regression analysis indicated that the P solubilised mainly through acidification. This study shows the possibility of using A. tubingensis SANRU for application in the management of P fertilisation.

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“…Based on the zone activity of different isolates further screening was carried by using Khandeparkar's selection ratio. Similarly, isolates were further analyzed for their ability to solubilize insoluble P using modified Pikovaskaya's agar medium according to Park et al [10] method.…”

Section: Identification Of the Bacteriamentioning

confidence: 99%

Role of Plant Growth Promoting Rhizobacteria in Accumulation of Heavy Metal in Metal Contaminated Soil

Jha¹,

Subrmanian²,

Mishra³

2017

ELSR

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Heavy metal pollution by industrial, agricultural and municipal wastewater is a frequent phenomenon. Bioremediation is a biological process for cleaning up of pollutants from the environment. In the present study, B. megaterium and P. aeruginosa isolated from a weed Suaeda nudiflora at chemical polluted site from Ankleshwar, Gujarat was used for bioremediation. These isolates were characterized for their ability of metal assimilation like phosphorus and iron, and on the basis of their best assimilation ability, the isolates were selected for heavy metal tolerance like zinc and lead. B. megaterium and P. aeruginosa with highest heavy metal tolerance up to 200 mgl -1 were inoculated in maize plants by test tube inoculation method and analyzed for the growth promotion efficacy in heavy metal polluted soil. The plant inoculated with isolates showed 20% higher plant height, 20% longer root length and 5% greater dry weight as compared to non-inoculated control. Further analyses of leaves were carried out for the accumulation of heavy metal in maize. Results of the study showed positive growth response of maize on heavy metal polluted soil in green house condition and also accumulate a significant amount of zinc (587 mg kg−1) and lead (227 mg kg−1) in plants. The study concluded that the metal mobilizing PGPR could be used as an effective inoculant for improving the phytoremediation in multi-metal polluted soil, as well as for the reclamation of heavy metal polluted soil.

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Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil

Cited by 225 publications

References 60 publications

Phytoextraction of heavy metal polluted soils using Sedum plumbizincicola inoculated with metal mobilizing Phyllobacterium myrsinacearum RC6b

Phytoextraction of heavy metal polluted soils using Sedum plumbizincicola inoculated with metal mobilizing Phyllobacterium myrsinacearum RC6b

Isolation and identification of a phosphate solubilising fungus from soil of a phosphate mine in Chaluse, Iran

Role of Plant Growth Promoting Rhizobacteria in Accumulation of Heavy Metal in Metal Contaminated Soil

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