Isolation of Phytol from the Hiziki Unsaponifiable Matter and Its Activating Effect on Pancreatic Lipase Activity

Abstract

In the previous report, the residual fraction (residue) after extraction of fucosterol from the unsaponifiable matter of Hizikia fusiformis (hiziki) was found to possess essentially the same remarkable accelerating activity on pig pancreatic lipase as that of the unsaponifiable matter. Hence, the isolation and identification of the active principles from the residue were attempted, and the activity of three activators was investigated.One of the activators isolated from the residue was identified as phytol, a diterpene, by means of TLC and IR and MS spectrometry, thus providing the first demonstration of phytol in hiziki. Phytol, a new lipase-activator, was found to exhibit a more remarkable (250 to 300%) stimulating action than the residue itself (150 to 170%) on lipolysis when tested with triacetin as substrate.Other two activators, A-3 and A-9, isolated from the residue were suggested to belong to terpenes and sterols, respectively, and A-3 showed an activating effect essentially similar to that of phytol. Accordingly, some other terpenes (from different sources) were compared of their lipase-activating activity, and farnesol proved to be the most active, essentially to the same degree as phytol, but nerol, (3-carotene, and a-tocopherol to be far less active. Thus, the requirement for the optimal isoprene units in the activator molecule was suggested. (Received March 11, 1974)

Effect of Unsaponifiable Lipid Components from the Red AlgaPorphyra teneraon Pancreatic Carboxylesterase Activity toward TriacetinIn Vitro

“…We have thus far studied the isolation and characterization of the activator, which stimulates markedly the hydrolysis of triacetin (TAesterase activity) by a porcine pancreatic lipase preparation, from the marine brown alga Hizikia fusiformis (hiziki).l -8) It has been found that phytol 6 ) isolated from hiziki is a typical activator of this kind, and recently that parathion 9 ) as well as retinol 10 ) exhibits a remarkable stimulating action, essentially similar to that observed with phytol, on the TAesterase activity.…”